pCDH- CMV- MCS- EF1- copGFP Plasmid


  • Model: PVT2334
  • 50 Units in Stock
Ask a question

Add to Cart:



Search name

pCDH-CMV-MCS-EF1-copGFP,Plasmid pCDH-CMV-MCS-EF1-copGFP,pCDH-CMV-MCS-EF1-copGFP vector


pCDH-CMV-MCS-EF1-copGFP Information

Promoter: EF-1 alpha core, CMV
Replicator: pUC ori, SV40 ori
Terminator: SV40 poly (A) signal
Plasmid classification: virus series, lentivirus cloning vector
Plasmid size: 7544bp
Prokaryotic resistance: Amp
Cloned strain: Stbl3
Culture conditions: 37 centigrade, aerobic LB
Expression host: mammalian cells
Induction mode: no induction, instantaneous expression
3'sequencing primers: primers designed according to sequence


pCDH-CMV-MCS-EF1-copGFP Description

The puromycin-resistance gene (puro) was cloned into pCDH-EF1-MCS-T2A-copGFP (A) and pCDH-MCS-T2A-copGFP-MSCV (B); and the copGFP gene (cG) was cloned into pCDH-EF1-MCS-T2A-Puro (C) and pCDH-MCS-T2A-Puro-MSCV (D). The resulting dual reporter constructs were packaged into pseudoviral particles followed by transduction into HT1080 cells. All constructs were also puromycin resistant (data not shown)




pCDH-CMV-MCS-EF1-copGFP Sequence


LOCUS       Exported                7544 bp ds-DNA     circular SYN 30-AUG-2016
DEFINITION  synthetic circular DNA
KEYWORDS    Untitled 3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7544)
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, August 30, 2016 from SnapGene Viewer 3.1.4
FEATURES             Location/Qualifiers
     source          1..7544
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             234..414
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2005..2208
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
     promoter        2347..2558
                     /note="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor 
     LTR             2571..2839
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             2898..3560
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
     misc_feature    3639..4227
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(4110..4121)
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
     LTR             4301..4534
                     /note="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
     polyA_signal    4606..4727
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      4746..4881
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     primer_bind     complement(4919..4935)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
     protein_bind    4943..4959
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4967..4997)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5012..5033
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5321..5909)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
     CDS             complement(6080..6940)
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     promoter        complement(6941..7045)
                     /note="AmpR promoter"
     primer_bind     7519..7535
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
No customer comments for the moment.

Add A Comment

Related Products


No products

Total $0.00

Prices don't include postage.