pCDH- EF1- luc2- T2A- Tdtomato Plasmid


  • Model: PVT2337
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pCDH-EF1-luc2-T2A-Tdtomato,Plasmid pCDH-EF1-luc2-T2A-Tdtomato,pCDH-EF1-luc2-T2A-Tdtomato vector


pCDH-EF1-luc2-T2A-Tdtomato Information

romoter: EF-1 alpha core

Replicator: pUC ori, SV40 ori

Terminator: SV40 poly (A) signal

Plasmid classification: virus series, lentivirus cloning vector

Plasmid size: 9565bp

Prokaryotic resistance: Amp

Cloned strain: Stbl3

Culture conditions: 37 centigrade, aerobic LB

Expression host: mammalian cells

Induction mode: no induction, instantaneous expression

5'sequencing primers: primers designed according to sequence

3'sequencing primers: primers designed according to sequence



pCDH-EF1-luc2-T2A-Tdtomato  Description



pCDH-EF1-luc2-T2A-Tdtomato Sequence

LOCUS       Exported                9565 bp ds-DNA     circular SYN 30-AUG-2016
DEFINITION  synthetic circular DNA
KEYWORDS    Untitled 2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9565)
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, August 30, 2016 from SnapGene Viewer 3.1.4
FEATURES             Location/Qualifiers
     source          1..9565
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(10..26)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
     promoter        500..604
                     /note="AmpR promoter"
     CDS             605..1465
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     rep_origin      1636..2224
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
     protein_bind    2512..2533
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2548..2578
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2586..2602
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2610..2626
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
     rep_origin      2664..2799
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     polyA_signal    2818..2939
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     LTR             3011..3244
                     /note="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
     misc_feature    3318..3906
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3424..3435
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
     CDS             complement(3913..5343)
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /note="mammalian codon-optimized"
     CDS             complement(5353..5406)
                     /product="2A peptide from Thosea asigna virus capsid 
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
     CDS             complement(541
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