pCDNA3.1-Zeo
Catalog No. PVT10674
Packing 2ug
pCDNA3.1-Zeo Information
Alias: pCDNA3.1(+)-Zeo
Promoter: CMV
Replicator: PUC
Terminator: bGH poly (a) signal
Plasmid classification: mammalian cell vector; protein overexpression plasmid
Plasmid size: 5015bp
Prokaryotic resistance: amp
Eukaryotic resistance: Zeo
Clone strain: DH5 α
Culture condition: 37 ℃
Expression host: mammalian cells
Culture condition: 37 ℃, aerobic, LB
Induction method: IPTG or lactose and its analogues
5 'sequencing primer: design according to sequence
3 'sequencing primer: design according to sequence
Plasmid properties
Plasmid host: mammalian cell
Purpose of plasmid: protein expression
Fragment type: gene CDs
Fragment species: empty body
Prokaryotic resistance: ampicillin
Eukaryotic resistance: bleomycin
pCDNA3.1-Zeo Description
pCDNA3.1-Zeo Sequence
LOCUS Exported 5015 bp ds-DNA circular SYN 18-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pCDNA3.1(+)-Zeo
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5015)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported 2016-9-18 from SnapGene Viewer 2.8.1
FEATURES Location/Qualifiers
source 1..5015
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 235..614
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 1028..1252
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin 1298..1726
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1740..2070
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 1921..2056
/note="SV40 ori"
/note="SV40 origin of replication"
promoter 2118..2165
/note="EM7 promoter"
/note="synthetic bacterial promoter "
CDS 2184..2558
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/note="BleoR"
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 2688..2809
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
primer_bind complement(2858..2874)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 2882..2898
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2906..2936)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 2951..2972
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(3260..3848)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4019..4879)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4880..4984)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc
961 agatatccag cacagtggcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca
1021 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
1081 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
1141 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
1201 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
1261 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
1321 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
1381 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
1441 gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc
1501 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
1561 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
1621 acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc
1681 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
1741 tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc
1801 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
1861 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
1921 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
1981 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
2041 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
2101 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
2161 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
2221 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
2281 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
2341 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
2401 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
2461 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
2521 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
2581 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
2641 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
2701 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
2761 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
2821 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
2881 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
2941 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
3001 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
3061 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
3121 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
3181 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
3241 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
3301 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
3361 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
3421 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
3481 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
3541 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
3601 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
3661 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
3721 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
3781 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
3841 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
3901 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
3961 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
4021 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
4081 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
4141 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
4201 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
4261 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
4321 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
4381 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
4441 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
4501 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
4561 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
4621 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
4681 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
4741 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
4801 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
4861 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
4921 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
4981 cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
Caution:
Product is for research use only!
