pcDNA-4mtD3cpv

$193.00

  • Model: PVT10530
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pCDNA-4mtD3cpv

Catalog No. PVT10530
Packing 2ug

 

pCDNA-4mtD3cpv Information

Function Other source plasmids

Promoter: CMV promoter

Replicator: ori, F1 ori, SV40 ori

Terminator: bGH poly (A) signal

Plasmid classification: gene library plasmid; other gene plasmids; other source plasmids.

Plasmid size: 7754bp

Prokaryotic resistance: ampicillin Amp (100 u g/ml)

Screening markers: neomycin Neo/G418

Cloned strains of Escherichia coli, DH10B and other Escherichia coli

Culture conditions: 37 centigrade, aerobic, LB

Expression host: 293T mammalian cells

Induction mode: no induction

 

pCDNA-4mtD3cpv Reference

Ca2+ indicators based on computationally redesigned calmodulin-peptide pairs.

 

pCDNA-4mtD3cpv Sequence

LOCUS       Exported                7754 bp ds-DNA     circular SYN 15-AUG-2017
DEFINITION  synthetic circular DNA
FEATURES             Location/Qualifiers
     source          1..7754
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(83..943)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(944..1048)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        1314..1693
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1694..1897
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        1942..1960
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1974..2060
                     /label=COX8a
                     /note="human COX subunit 8a"
     misc_feature    2069..4325
                     /label=4mtD3cpv
                     /note="4mtD3cpv second generation cameleon (calcium sensor)
                     targeted to mitochondria"
     CDS             3804..4322
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     promoter        complement(4386..4404)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    4430..4654
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4700..5128
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5142..5471
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5322..5457
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5538..6332
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6506..6627
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(7078..7666)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
       61 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
      121 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
      181 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
      241 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
      301 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
      361 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
      421 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
      481 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
      541 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
      601 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
      661 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
      721 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
      781 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
      841 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
      901 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
      961 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
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