pCMVβ-p300-myc

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  • Model: PVT10229
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pCMVβ-p300-myc

PVT10229    2ug

 

pCMVβ-p300-myc Back Bones Information

Vector: pCMVβ

Carrier company:--

Vector resistance: Ampicillin

Empty body size: 4800bp

Carrier modification:--

Vector type: mammalian cell expression

Screening marker:--

Copy number : high copy

Growth condition: 37 ℃
 

Insert gene information

Insert gene name: P300

Species origin: H. sapiens (human)

Insert size:--

Gene mutation:--

GenBank number--

Promoter--

Tag / fusion protein: myc (inserted C terminal)

Cloning method: restriction endonuclease

5 'clonal site:--

3 'clonal site:--

5 'sequencing primer: CMV FWD

3 'sequencing primer--

 

pCMVβ-p300-myc Sequence
 

LOCUS       pCMV-beta-p300-myc               11893 bp ds-DNA     circular SYN 17-MAY-2019
DEFINITION  Mammalian expression of human p300 with myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-beta-p300-myc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11893)
  TITLE     p300 plasmids
REFERENCE   2  (bases 1 to 11893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 17, 2019 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11893
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(30..52)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     152..171
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     385..404
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        694..712
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     694..711
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     enhancer        736..1039
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1040..1243
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1194..1214
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1240..1264
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1345..1361
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1345..1361
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             4572..6422
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      6186..6195
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             8673..8702
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          8761..9333
                     /label=-beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(9465..9484)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_site      9530..9585
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     primer_bind     complement(9584..9603)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9904..9921)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10075..10663)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10155..10174)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10834..11694)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11457..11476
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11695..11799)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11867..11885
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
       61 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
      121 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
      181 caccattcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt
      241 gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag
      301 tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa
      361 gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc
      421 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatctggc tagcgatgac
      481 cctgctgatt ggttcgctga ccatttccgg gtgcgggacg gcgttaccag aaactcagaa
      541 ggttcgtcca accaaaccga ctctgacggc agtttacgag agagatgata gggtctgctt
      601 cagtaagcca gatgctacac aattaggctt gtacatattg tcgttagaac gcggctacaa
      661 ttaatacata accttatgta tcatacacat acgatttagg tgacactata gaatacacgg
      721 aattcgagct cgccccgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      781 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      841 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      901 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      961 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     1021 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     1081 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     1141 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     1201 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     1261 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
     1321 cagcctccgg gggatcgcgg ccgctctaga actagtggat cccccgggcc gaagaagaga
     1381 tttcctgagg attctggttt tcctcgcttg tatctccgaa agaattaaaa atggccgaga
     1441 atgtggtgga accggggccg ccttcagcca agcggcctaa actctcatct ccggccctct
     1501 cggcgtccgc cagcgatggc acagattttg gctctctatt tgacttggag cacgacttac
     1561 cagatgaatt aatcaactct acagaattgg gactaaccaa tggtggtgat attaatcagc
     1621 ttcagacaag tcttggcatg gtacaagatg cagcttctaa acataaacag ctgtcagaat
     1681 tgctgcgatc tggtagttcc cctaacctca atatgggagt tggtggccca ggtcaagtca
     1741 tggccagcca ggcccaacag agcagtcctg gattaggttt gataaatagc atggtcaaaa
     1801 gcccaatgac acaggcaggc ttgacttctc ccaacatggg gatgggcact agtggaccaa
     1861 atcagggtcc tacgcagtca acaggtatga tgaacagtcc agtaaatcag cctgccatgg
     1921 gaatgaacac agggatgaat gcgggcatga atcctggaat gttggctgca ggcaatggac
     1981 aagggataat gcctaatcaa gtcatgaacg gttcaattgg agcaggccga gggcgacaga
     2041 atatgcagta cccaaaccca ggcatgggaa gtgctggcaa cttactgact gagcctcttc
     2101 agcagggctc tccccagatg ggaggacaaa caggattgag aggcccccag cctcttaaga
     2161 tgggaatgat gaacaacccc aatccttatg gttcaccata tactcagaat cctggacagc
     2221 agattggagc cagtggcctt ggtctccaga ttcagacaaa aactgtacta tcaaataact
     2281 tatctccatt tgctatggac aaaaaggcag ttcctggtgg aggaatgccc aacatgggtc
     2341 aacagccagc cccgcaggtc cagcagccag gtctggtgac tccagttgcc caagggatgg
     2401 gttctggagc acatacagct gatccagaga agcgcaagct catccagcag cagcttgttc
     2461 tccttttgca tgctcacaag tgccagcgcc gggaacaggc caatggggaa gtgaggcagt
     2521 gcaaccttcc ccactgtcgc acaatgaaga atgtcctaaa ccacatgaca cactgccagt
     2581 caggcaagtc ttgccaagtg gcacactgtg catcttctcg acaaatcatt tcacactgga
     2641 agaattgtac aagacatgat tgtcctgtgt gtctccccct caaaaatgct ggtgataaga
     2701 gaaatcaaca gccaattttg actggagcac ccgttggact tggaaatcct agctctctag
     2761 gggtgggtca acagtctgcc cccaacctaa gcactgttag tcagattgat cccagctcca
     2821 tagaaagagc ctatgcagct cttggactac cctatcaagt aaatcagatg ccgacacaac
     2881 cccaggtgca agcaaagaac cagcagaatc agcagcctgg gcagtctccc caaggcatgc
     2941 ggcccatgag caacatgagt gctagtccta tgggagtaaa tggaggtgta ggagttcaaa
     3001 cgccgagtct tctttctgac tcaatgttgc attcagccat aaattctcaa aacccaatga
     3061 tgagtgaaaa tgccagtgtg ccctccctgg gtcctatgcc aacagcagct caaccatcca
     3121 ctactggaat tcggaaacag tggcacgaag atattactca ggatcttcga aatcatcttg
     3181 ttcacaaact cgtccaagcc atatttccta cgccggatcc tgctgcttta aaagacagac
     3241 ggatggaaaa cctagttgca tatgctcgga aagttgaagg ggacatgtat gaatctgcaa
     3301 acaatcgagc ggaatactac caccttctag ctgagaaaat ctataagatc cagaaagaac
     3361 tagaagaaaa acgaaggacc agactacaga agcagaacat gctaccaaat gctgcaggca
     3421 tggttccagt ttccatgaat ccagggccta acatgggaca gccgcaacca ggaatgactt
     3481 ctaatggccc tctacctgac ccaagtatga tccgtggcag tgtgccaaac cagatgatgc
     3541 ctcgaataac tccacaatct ggtttgaatc aatttggcca gatgagcatg gcccagcccc
     3601 ctattgtacc ccggcaaacc cctcctcttc agcaccatgg acagttggct caacctggag
     3661 ctctcaaccc gcctatgggc tatgggcctc gtatgcaaca gccttccaac cagggccagt
     3721 tccttcctca gactcagttc ccatcacagg gaatgaatgt aacaaatatc cctttggctc
     3781 cgtccagcgg tcaagctcca gtgtctcaag cacaaatgtc tagttcttcc tgcccggtga
     3841 actctcctat aatgcctcca gggtctcagg ggagccacat tcactgtccc cagcttcctc
     3901 aaccagctct tcatcagaat tcaccctcgc ctgtacctag tcgtaccccc acccctcacc
     3961 atactccccc aagcataggg gctcagcagc caccagcaac aacaattcca gcccctgttc
     4021 ctacacctcc tgccatgcca cctgggccac agtcccaggc tctacatccc cctccaaggc
     4081 agacacctac accaccaaca acacaacttc cccaacaagt gcagccttca cttcctgctg
     4141 caccttctgc tgaccagccc cagcagcagc ctcgctcaca gcagagcaca gcagcgtctg
     4201 ttcctacccc aacagcaccg ctgcttcctc cgcagcctgc aactccactt tcccagccag
     4261 ctgtaagcat tgaaggacag gtatcaaatc ctccatctac tagtagcaca gaagtgaatt
     4321 ctcaggccat tgctgagaag cagccttccc aggaagtgaa gatggaggcc aaaatggaag
     4381 tggatcaacc agaaccagca gatactcagc cggaggatat ttcagagtct aaagtggaag
     4441 actgtaaaat ggaatctacc gaaacagaag agagaagcac tgagttaaaa actgaaataa
     4501 aagaggagga agaccagcca agtacttcag ctacccagtc atctccggct ccaggacagt
     4561 caaagaaaaa gattttcaaa ccagaagaac tacgacaggc actgatgcca actttggagg
     4621 cactttaccg tcaggatcca gaatcccttc cctttcgtca acctgtggac cctcagcttt
     4681 taggaatccc tgattacttt gatattgtga agagccccat ggatctttct accattaaga
     4741 ggaagttaga cactggacag tatcaggagc cctggcagta tgtcgatgat atttggctta
     4801 tgttcaataa tgcctggtta tataaccgga aaacatcacg ggtatacaaa tactgctcca
     4861 agctctctga ggtctttgaa caagaaattg acccagtgat gcaaagcctt ggatactgtt
     4921 gtggcagaaa gttggagttc tctccacaga cactgtgttg ctacggcaaa cagttgtgca
     4981 caatacctcg tgatgccact tattacagtt accagaacag gtatcatttc tgtgagaagt
     5041 gtttcaatga gatccaaggg gagagcgttt ctttggggga tgacccttcc cagcctcaaa
     5101 ctacaataaa taaagaacaa ttttccaaga gaaaaaatga cacactggat cctgaactgt
     5161 ttgttgaatg tacagagtgc ggaagaaaga tgcatcagat ctgtgtcctt caccatgaga
     5221 tcatctggcc tgctggattc gtctgtgatg gctgtttaaa gaaaagtgca cgaactagga
     5281 aagaaaataa gttttctgct aaaaggttgc catctaccag acttggcacc tttctagaga
     5341 atcgtgtgaa tgactttctg aggcgacaga atcaccctga gtcaggagag gtcactgtta
     5401 gagtagttca tgcttctgac aaaaccgtgg aagtaaaacc aggcatgaaa gcaaggtttg
     5461 tggacagtgg agagatggca gaatcctttc cataccgaac caaagccctc tttgcctttg
     5521 aagaaattga tggtgttgac ctgtgcttct ttggcatgca tgttcaagag tatggctctg
     5581 actgccctcc acccaaccag aggagagtat acatatctta cctcgatagt gttcatttct
     5641 tccgtcctaa atgcttgagg actgcagtct atcatgaaat cctaattgga tatttagaat
     5701 atgtcaagaa attaggttac acaacagggc atatttgggc atgtccacca agtgagggag
     5761 atgattatat cttccattgc catcctcctg accagaagat acccaagccc aagcgactgc
     5821 aggaatggta caaaaaaatg cttgacaagg ctgtatcaga gcgtattgtc catgactaca
     5881 aggatatttt taaacaagct actgaagata gattaacaag tgcaaaggaa ttgccttatt
     5941 tcgagggtga tttctggccc aatgttctgg aagaaagcat taaggaactg gaacaggagg
     6001 aagaagagag aaaacgagag gaaaacacca gcaatgaaag cacagatgtg accaagggag
     6061 acagcaaaaa tgctaaaaag aagaataata agaaaaccag caaaaataag agcagcctga
     6121 gtaggggcaa caagaagaaa cccgggatgc ccaatgtatc taacgacctc tcacagaaac
     6181 tatatgccac catggagaag cataaagagg tcttctttgt gatccgcctc attgctggcc
     6241 ctgctgccaa ctccctgcct cccattgttg atcctgatcc tctcatcccc tgcgatctga
     6301 tggatggtcg ggatgcgttt ctcacgctgg caagggacaa gcacctggag ttctcttcac
     6361 tccgaagagc ccagtggtcc accatgtgca tgctggtgga gctgcacacg cagagccagg
     6421 accgctttgt ctacacctgc aatgaatgca agcaccatgt ggagacacgc tggcactgta
     6481 ctgtctgtga ggattatgac ttgtgtatca cctgctataa cactaaaaac catgaccaca
     6541 aaatggagaa actaggcctt ggcttagatg atgagagcaa caaccagcag gctgcagcca
     6601 cccagagccc aggcgattct cgccgcctga gtatccagcg ctgcatccag tctctggtcc
     6661 atgcttgcca gtgtcggaat gccaattgct cactgccatc ctgccagaag atgaagcggg
     6721 ttgtgcagca taccaagggt tgcaaacgga aaaccaatgg cgggtgcccc atctgcaagc
     6781 agctcattgc cctctgctgc taccatgcca agcactgcca ggagaacaaa tgcccggtgc
     6841 cgttctgcct aaacatcaag cagaagctcc ggcagcaaca gctgcagcac cgactacagc
     6901 aggcccaaat gcttcgcagg aggatggcca gcatgcagcg gactggtgtg gttgggcagc
     6961 aacagggcct cccttccccc actcctgcca ctccaacgac accaactggc caacagccaa
     7021 ccaccccgca gacgccccag cccacttctc agcctcagcc tacccctccc aatagcatgc
     7081 caccctactt gcccaggact caagctgctg gccctgtgtc ccagggtaag gcagcaggcc
     7141 aggtgacccc tccaacccct cctcagactg ctcagccacc ccttccaggg cccccacctg
     7201 cagcagtgga aatggcaatg cagattcaga gagcagcgga gacgcagcgc cagatggccc
     7261 acgtgcaaat ttttcaaagg ccaatccaac accagatgcc cccgatgact cccatggccc
     7321 ccatgggtat gaacccacct cccatgacca gaggtcccag tgggcatttg gagccaggga
     7381 tgggaccgac agggatgcag caacagccac cctggagcca aggaggattg cctcagcccc
     7441 agcaactaca gtctgggatg ccaaggccag ccatgatgtc agtggcccag catggtcaac
     7501 ctttgaacat ggctccacaa ccaggattgg gccaggtagg tatcagccca ctcaaaccag
     7561 gcactgtgtc tcaacaagcc ttacaaaacc ttttgcggac tctcaggtct cccagctctc
     7621 ccctgcagca gcaacaggtg cttagtatca ttcacgccaa cccccagctg ttggctgcat
     7681 tcatcaagca gcgggctgcc aagtatgcca actctaatcc acaacccatc cctgggcagc
     7741 ctggcatgcc ccaggggcag ccagggctac agccacctac catgccaggt cagcaggggg
     7801 tccactccaa tccagccatg cagaacatga atccaatgca ggcgggcgtt cagagggctg
     7861 gcctgcccca gcagcaacca cagcagcaac tccagccacc catgggaggg atgagccccc
     7921 aggctcagca gatgaacatg aaccacaaca ccatgccttc acaattccga gacatcttga
     7981 gacgacagca aatgatgcaa cagcagcagc aacagggagc agggccagga ataggccctg
     8041 gaatggccaa ccataaccag ttccagcaac cccaaggagt tggctaccca ccacagcagc
     8101 agcagcggat gcagcatcac atgcaacaga tgcaacaagg aaatatggga cagataggcc
     8161 agcttcccca ggccttggga gcagaggcag gtgccagtct acaggcctat cagcagcgac
     8221 tccttcagca acagatgggg tcccctgttc agcccaaccc catgagcccc cagcagcata
     8281 tgctcccaaa tcaggcccag tccccacacc tacaaggcca gcagatccct aattctctct
     8341 ccaatcaagt gcgctctccc cagcctgtcc cttctccacg gccacagtcc cagccccccc
     8401 actccagtcc ttccccaagg atgcagcctc agccttctcc acaccacgtt tccccacaga
     8461 caagttcccc acatcctgga ctggtagctg cccaggccaa ccccatggaa caagggcatt
     8521 ttgccagccc ggaccagaat tcaatgcttt ctcagcttgc tagcaatcca ggcatggcaa
     8581 acctccatgg tgcaagcgcc acggacctgg gactcagcac cgataactca gacttgaatt
     8641 caaacctctc acagagtaca ctagacatac acgaacaaaa acttatttct gaagaagacc
     8701 tatagaagct tatgcataga tctatgcata gatctatgca tagatcctga gaacttcagg
     8761 gtgagtttgg ggacccttga ttgttctttc tttttcgcta ttgtaaaatt catgttatat
     8821 ggagggggca aagttttcag ggtgttgttt agaatgggaa gatgtccctt gtatcaccat
     8881 ggaccctcat gataattttg tttctttcac tttctactct gttgacaacc attgtctcct
     8941 cttattttct tttcattttc tgtaactttt tcgttaaact ttagcttgca tttgtaacga
     9001 atttttaaat tcacttttgt ttatttgtca gattgtaagt actttctcta atcacttttt
     9061 tttcaaggca atcagggtat attatattgt acttcagcac agttttagag aacaattgtt
     9121 ataattaaat gataaggtag aatatttctg catataaatt ctggctggcg tggaaatatt
     9181 cttattggta gaaacaacta caccctggtc atcatcctgc ctttctcttt atggttacaa
     9241 tgatatacac tgtttgagat gaggataaaa tactctgagt ccaaaccggg cccctctgct
     9301 aaccatgttc atgccttctt ctctttccta cagctcctgg gcaacgtgct ggttgttgtg
     9361 ctgtctcatc attttggcaa agaattcact cctcaggtgc aggctgccta tcagaaggtg
     9421 gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc
     9481 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa
     9541 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
     9601 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
     9661 gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa
     9721 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt
     9781 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgct
     9841 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     9901 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     9961 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    10021 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    10081 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    10141 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    10201 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    10261 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    10321 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    10381 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    10441 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    10501 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    10561 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    10621 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    10681 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    10741 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    10801 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    10861 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    10921 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    10981 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    11041 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    11101 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    11161 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    11221 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    11281 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    11341 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    11401 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    11461 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    11521 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    11581 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    11641 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    11701 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    11761 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    11821 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
    11881 gaggcccttt cgt
//

Caution:
1.  This product is FOR RESEARCH USE ONLY!
2.  The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.

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