pCMV- PKA

$356.00

  • Model: PVTH0007
  • 50 Units in Stock
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pCMV-PKA

Cat No. PVTH0007

 

pCMV-PKA Alternative Name

pCMV-PKA, pCMV-PKA vector, pCMV-PKA plasmid, pCMV-PKA map, pCMV-PKA Sequence

 

pCMV-PKA  Information

Carrier name: pCMV-PKA

Plasmid type: mammalian expression vector; gene carrier; signaling pathway reporter vector

High copy / low copy: high copy

Cloning method: restrictive endonuclease, polyclonal site

Promoter: CMV

Carrier size: 5 KB

5'sequencing primers and sequences: --

3'sequencing primers and sequences: --

Carrier label: no

Carrier resistance: kanamycin

Screening markers: Neomycin

Clone strain: HB101, DH5alpha; JM109 (single strand DNA replication).

Host cells (lines): conventional cell lines, such as 293, Hela, etc.

Note: the pCMV-PKA vector is the expression vector of the mouse protein kinase A (PKA); the CMV promoter drives the overexpression of the PKA gene; it is used to study the cell PKA signaling pathway, and the kinase quantitative analysis kit can quantitatively analyze the function of the protein in the signal pathway.

Stability: transient or stable expression

Composition / inducible type: composition

Virus / non virus: non virus

 

pCMV-PKA Description

pCMV-PKA is designed for the constitutive expression of Protein Kinase A (PKA) (mouse) in mammalian cells. It can also be used to study the effects of a given stimulus on the PKA signaling pathway. In conjunction with Clontech’s In Vivo Kinase Assay Kits, pCMV-PKA is an ideal positive control for studying a gene or molecule and its affect on kinase activation in the PKA pathway. cAMP, a ubiquitous second messenger, regulates expression of cellular functions including metabolism, cell proliferation, and neuronal signaling via activation of the PKA-dependent phosphorylation of the CREB transcription factor (1–3). pCMV-PKA contains the gene encoding the catalytic subunit of PKA, which is driven by the human cytomegalovirus (CMV) promoter. SV40 polyadenylation signals downstream of the PKA gene direct proper processing of the 3’ end of the mRNA. The vector backbone also contains an SV40 origin for replication in mammalian cells expressing the SV40 T-antigen. A neomycin resistance cassette (Neor)—consisting of the SV40 early promoter, the neomycin/kanamycin resistance gene of Tn5, and polyadenylation signals from the Herpes simplex virus thymidine kinase (HSV TK) gene—allows stably transfected eukaryotic cells to be selected using G418 (4). A bacterial promoter upstream of this cassette expresses kanamycin resistance in E. coli. The pCMV-PKA backbone also provides a pUC origin of replication for propagation in E. coli and an f1 origin for single-stranded DNA production. The recombinant vector can be transfected into mammalian cells using any standard method.

pCMV-PKA-vector

 

pCMV-PKA Sequence

 

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