pCMV-HA

$182.00

  • Model: PVT27427
  • 50 Units in Stock
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pCMV-HA

PVT27427  2ug
 

pCMV-HA Information
Vector Backbone:    pCMV-HA
Tag:    HA(N-term)
Backbone manufacturer:    Clontech
Vector type:    Mammalian Expression
Backbone size w/o insert:    3800bp
Cloning site 5':    N/A
Site destroyed during cloning:    N/A
Cloning site 3':    N/A
Site destroyed during cloning:    N/A
Bacterial resistance(s):    Ampicillin
Growth strain(s):    Top10
Growth temperature:    37℃
High or low copy:    High Copy
Plasmid description:    
Selectable markers:    None


pCMV-HA Description
pCMV-HA is a mammalian cell expression system vector and size is 3782bp. It is used to express the N-terminal ha labeled protein in mammalian cells and resistance is Amp.

pCMV-HA-plasmid

 

pCMV-HA Map

 

pCMV-HA-map

 

pCMV-HA Sequene

LOCUS       pCMV-HA 3782 bp  DNA SYN
DEFINITION  pCMV-HA
ACCESSION  
KEYWORDS   
SOURCE     
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="pCMV-HA"
                     /mol_type="other DNA"
     misc_feature    31..318
                     /label="CAG_enhancer"
     promoter        36..528
                     /label="CMV_immearly_promoter"
     misc_feature    485..505
                     /label="CMV_fwd_primer"
     promoter        486..555
                     /label="CMV_promoter"
     promoter        498..617
                     /label="CMV2_promoter"
     misc_feature    529..548
                     /label="pCEP_fwd_primer"
     misc_feature    531..555
                     /label="LNCX_primer"
     misc_feature    631..657
                     /label="pCMV_primer"
     misc_feature    672..768
                     /label="modSV40_late_16s_int"
     misc_feature    672..702
                     /label="SV40_late_19s_int"
     terminator      complement(931..1050)
                     /label="SV40_PA_terminator"
     misc_feature    complement(945..964)
                     /label="EBV_rev_primer"
     promoter        complement(1162..1180)
                     /label="M13_reverse_primer"
     misc_feature    complement(1179..1201)
                     /label="M13_pUC_rev_primer"
     promoter        complement(1215..1244)
                     /label="lac_promoter"
     gene            complement(2327..3187)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(2327..3187)
                     /label="ORF frame 2"
     promoter        complement(3229..3257)
                     /label="AmpR_promoter"
     misc_feature    complement(3416..3438)
                     /label="pGEX_3_primer"
     misc_feature    complement(3637..3780)
                     /label="lacZ_a"
     misc_feature    3751..3773
                     /label="M13_pUC_fwd_primer"
     promoter        3766..3782
                     /label="M13_forward20_primer"
ORIGIN
    1 GAGTTCGAGC TTGCATGCCT GCAGGTCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC
   61 TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG
  121 CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG
  181 GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA
  241 TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC
  301 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG
  361 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG
  421 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA
  481 TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA
  541 GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGACAC
  601 CGGGACCGAT CCAGCCTCCG GACTCTAGAG GATCCGGTAC TAGAGGAACT GAAAAACCAG
  661 AAAGTTAACT GGTAAGTTTA GTCTTTTTGT CTTTTATTTC AGGTCCCGGA TCCGGTGGTG
  721 GTGCAAATCA AAGAACTGCT CCTCAGTGGA TGTTGCCTTT ACTTCTAGGC CTGTACGGAA
  781 GTGTTACTTC TGCTCTAAAA GCTGCGGAAT TGTACCCGCG GGCCCACCAT GTACCCATAC
  841 GATGTTCCAG ATTACGCTCT TATGGCCATG GAGGCCCGAA TTCGGTCGAC CGAGATCTCT
  901 CGAGGTACCG CGGCCGCGGG GATCCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA
  961 CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT
1021 TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA
1081 TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTCGGA TCCTCTAGAG TCGATCTGCA
1141 GGCATGCTAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC
1201 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT
1261 GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC
1321 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG
1381 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG
1441 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG
1501 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC
1561 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC
1621 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC
1681 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT
1741 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG
1801 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT
1861 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG
1921 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT
1981 GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC
2041 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA
2101 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG
2161 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA
2221 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA
2281 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA
2341 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC
2401 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA
2461 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA
2521 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT
2581 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG
2641 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC
2701 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG
2761 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG
2821 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT
2881 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT
2941 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC
3001 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC
3061 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG
3121 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA
3181 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA
3241 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC
3301 CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA
3361 ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT
3421 GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC
3481 AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG
3541 CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG CACAGATGCG
3601 TAAGGAGAAA ATACCGCATC AGGCGCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG
3661 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA
3721 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA
3781 GT
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