pCMV-HA-SIRT1(T95A/D204G/T209I/L436H/E690G) Plasmid 

Catalog No. PVTB00126-2a

Vector Backbone pCMV-HA

Backbone manufacturer Clontech

Vector type Mammalian Expression

Backbone size w/o insert 3800bp

Cloning site 5 Sal I

Site destroyed during cloning NO

Cloning site 3 Not I

Bacterial resistance Ampicillin

Growth strain DH5alpha

Growth temperature 37 Centigrade

High or low copy High Copy

Selectable markers None

Background Mammalian Expression vector of Homo sapiens sirtuin 1(five points mutation T95A/D204G/T209I/L436H/E690G) with N terminal HA tag

Gene Information

Alternative Gene Name SIR2; SIR2L1; SIR2alpha

Insert Gene name SIRT1(T95A/D204G/T209I/L436H/E690G)

Insert size 2244bp

Accessions NM_012238

Tag HA(N-term)

Species Homo sapiens (human)

Gene ID 23411