pCMV-HA-Ub
PVT7216 2ug
pCMV-HA-Ub Information
Alternative name: 1 × UB
Promoter: CMV
Replicator: pUC
Terminator: SV40 poly (a) signal
Plasmid classification: gene library plasmids; human plasmids; ubiquitin series plasmids
Plasmid size: 3997bp
Plasmid label: n-HA
Prokaryotic resistance: Amp
Filter tag: None
Clone strain: DH5a
Culture condition: 37 ℃
Expression host: Mammalian cells
Induction mode: no induction required
5 'sequencing primer: cmv-f: cgcaaatggggtgggtgggtg
3 'sequencing primer: sv40-polya-r: gaaatttgatgctattgc
Vector host: mammalian cells
Application of vector: protein expression
Gene species: Human
Gene type: ORF
Prokaryotic resistance: Amp
Species human(H. sapiens)
pCMV-HA-Ub Description
There are four human ubiquitin genes, which encode the same protein, called ubiquitin molecule. The mRNA levels of these four genes are slightly different, but the protein sequences are the same. Among them, ubiquitin C (UBC) and ubiquitin B (UBB) genes are the most common. UBC mRNA encodes six repeats of ubiquitin molecule, and UBB encodes four repeats of ubquitin molecule. Therefore, ha-ub cloned from foreign sources only clone one UB molecule, that is, only 76 amino acids.
HA-ub(n),n=1.2.3.4.5.6.....
Homo sapiens ubiquitin C (UBC), mRNA，NCBI Reference Sequence: NM_021009.6
pCMV-HA-Ub Site
pCMV-HA-Ub Sequence
LOCUS Exported 3997 bp ds-DNA circular SYN 11-MAR-2017
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pCMV-HA-UB
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3997)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Saturday, March 11, 2017
FEATURES Location/Qualifiers
source 1..3997
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 27..330
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 331..534
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
intron 672..768
/note="SV40 intron"
/note="modified SV40 intron with splice donor and acceptor
sites"
CDS 832..858
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/note="HA"
/translation="YPYDVPDYA"
misc_feature 886..1113
/note="UB"
polyA_signal 1136..1270
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
primer_bind complement(1381..1397)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 1405..1421
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1429..1459)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 1474..1495
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(1783..2371)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2542..3402)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3403..3507)
/gene="bla"
/note="AmpR promoter"
primer_bind 3981..3997
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat gtacccatac
841 gatgttccag attacgctct tatggccatg gaggcccgaa ttcgcatgca gatcttcgtg
901 aagaccctga ctggtaagac catcactctc gaagtggagc cgagtgacac cattgagaat
961 gtcaaggcaa agatccaaga caaggaaggc atccctcctg accagcagag gttgatcttt
1021 gctgggaaac agctggaaga tggacgcacc ctgtctgact acaacatcca gaaagagtcc
1081 accctgcacc tggtcctccg tctcagaggt gggagtgcgg taccgcggcc gcggggatcc
1141 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
1201 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
1261 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
1321 ggaggttttt tcggatcctc tagagtcgat ctgcaggcat gctagcttgg cgtaatcatg
1381 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
1441 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
1501 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
1561 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
1621 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
1681 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
1741 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
1801 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
1861 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
1921 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
1981 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
2041 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
2101 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
2161 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
2221 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
2281 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
2341 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
2401 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
2461 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
2521 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
2581 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
2641 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
2701 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
2761 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
2821 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
2881 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
2941 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
3001 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
3061 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
3121 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
3181 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
3241 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
3301 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
3361 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
3421 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
3481 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
3541 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
3601 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
3661 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
3721 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
3781 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
3841 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
3901 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
3961 gttttcccag tcacgacgtt gtaaaacgac ggccagt
//
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.
Search name
pCMV-HA-Ub,Plasmid pCMV-HA-Ub,pCMV-HA-Ub vector
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