pCP-Cre

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pCP-Cre,Plasmid pCP-Cre,pCP-Cre vector

pCP-Cre Decription

PCP-Cre is transformed from pCP20, including its Ori and Cm resistance genes. The Cre gene is controlled by the weakened LacUV5 promoter, and the resistance genes in the genome can be eliminated overnight after induction by 1mM ITPG. (the test tube is long to OD600=0.6-0.8 to begin to induce, the LB plate is crossed after night, and the single colony is selected to verify the loss of resistance). This plasmid is a low copy plasmid. When the plasmid is used in the kit, the 10ml bacteria extract, 1 columns, and the 50ul water is used to collect the plasmid, and the strip can be seen.

Use:CRE plasmid