pCS2- EGFP- EB3 Plasmid

$136.00

  • Model: PVT7208
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pCS2-EGFP-EB3

Search name

pCS2-EGFP-EB3,Plasmid pCS2-EGFP-EB3,pCS2-EGFP-EB3 vector

 

pCS2-EGFP-EB3 Information

Promoter: CMV IE94 promoter

Plasmid size: 5634bp

Plasmid label: N-EGFP

Prokaryotic resistance: ampicillin Amp

Species human(H. sapiens)

 

pCS2-EGFP-EB3 Description

PCS2-EGFP-EB3 is a human gene overexpression plasmid.

 

pCS2-EGFP-EB3 Sequence

LOCUS       Exported                5634 bp ds-DNA     circular SYN 11-OCT-2017
DEFINITION  synthetic circular DNA
KEYWORDS    pCS2-EGFP-EB3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   .
  TITLE     Direct Submission
 
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        38..1022
                     /note="CMV IE94 promoter"
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        1057..1075
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             1107..1823
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1830..2675
                     /codon_start=1
                     /note="EB3"
                     /translation="MAVNVYSTSVTSENLSRHDMLAWVNDSLHLNYTKIEQLCSGAAYC
                     QFMDMLFPGCVHLRKVKFQAKLEHEYIHNFKVLQAAFKKMGVDKIIPVEKLVKGKFQDN
                     FEFIQWFKKFFDANYDGKDYNPLLARQGQDVAPPPNPGDQIFNKSKKLIGTAVPQRTSP
                     TGPKNMQTSGRLSNVAPPCILRKNPPSARNGGHEADAQILELNQQLLDLKLTVDGLEKE
                     RDFYFSKLRDIELICQEHESENSPVISGIIGILYATEEGFAPPEDDEIEEHQQEDQDEY
                     "
     polyA_signal    2705..2839
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3393..3981)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4152..5012)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5013..5117)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      complement(5143..5598)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ggcgatcggt gcgggcctct tcgctattac gccagtcgac catagccaat tcaatatggc
       61 gtatatggac tcatgccaat tcaatatggt ggatctggac ctgtgccaat tcaatatggc
      121 gtatatggac tcgtgccaat tcaatatggt ggatctggac cccagccaat tcaatatggc
      181 ggacttggca ccatgccaat tcaatatggc ggacttggca ctgtgccaac tggggagggg
      241 tctacttggc acggtgccaa gtttgaggag gggtcttggc cctgtgccaa gtccgccata
      301 ttgaattggc atggtgccaa taatggcggc catattggct atatgccagg atcaatatat
      361 aggcaatatc caatatggcc ctatgccaat atggctattg gccaggttca atactatgta
      421 ttggccctat gccatatagt attccatata tgggttttcc tattgacgta gatagcccct
      481 cccaatgggc ggtcccatat accatatatg gggcttccta ataccgccca tagccactcc
      541 cccattgacg tcaatggtct ctatatatgg tctttcctat tgacgtcata tgggcggtcc
      601 tattgacgta tatggcgcct cccccattga cgtcaattac ggtaaatggc ccgcctggct
      661 caatgcccat tgacgtcaat aggaccaccc accattgacg tcaatgggat ggctcattgc
      721 ccattcatat ccgttctcac gccccctatt gacgtcaatg acggtaaatg gcccacttgg
      781 cagtacatca atatctatta atagtaactt ggcaagtaca ttactattgg aaggacgcca
      841 gggtacattg gcagtactcc cattgacgtc aatggcggta aatggcccgc gatggctgcc
      901 aagtacatcc ccattgacgt caatggggag gggcaatgac gcaaatgggc gttccattga
      961 cgtaaatggg cggtaggcgt gcctaatggg aggtctatat aagcaatgct cgtttaggga
     1021 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
     1081 agctacttgt tctttttgca ggatccatgg tgagcaaggg cgaggagctg ttcaccgggg
     1141 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     1201 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     1261 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     1321 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     1381 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     1441 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     1501 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     1561 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     1621 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     1681 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     1741 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     1801 tcggcatgga cgagctgtac aaggaattca tggctgtcaa tgtgtactcc acttctgtga
     1861 ccagtgagaa tctgagtcgc catgatatgc ttgcatgggt caatgactct ctgcacctca
     1921 attataccaa gattgaacag ctctgttcag gggcagccta ctgccagttc atggacatgc
     1981 tcttccctgg ctgtgttcac ttgaggaagg tcaagttcca ggccaaacta gaacacgagt
     2041 acatccacaa cttcaaggtg ctgcaagcag ctttcaagaa gatgggtgtt gacaaaatca
     2101 ttcccgtaga gaagttagtg aaaggaaaat tccaagataa ttttgagttt atacagtggt
     2161 ttaagaaatt ctttgacgca aactatgatg gaaaggatta caaccctctg ctggcgcggc
     2221 agggccagga cgtagcacca cctcctaacc caggtgatca gatcttcaac aaatccaaga
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