Catalog No. PVT11173
Packing 2ug
LOCUS Exported 12038 bp ds-DNA circular SYN 04-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..12038
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 115..367
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS 728..1033
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
CDS complement(1089..1115)
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
promoter complement(1131..3121)
/label=Ubi promoter
/note="maize polyubiquitin gene promoter"
misc_feature 3362..3386
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 4685..5314
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 5748..6815
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
MRYRNLIEGEASAGS"
rep_origin 6881..7075
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 7419..7559
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(7745..8333)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8420..9214)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 9639..9663
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 9741..9915
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(9955..10980)
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
K"
promoter complement(11047..11724)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
ORIGIN
1 gaattcgagc tcggtacccc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
61 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tcccgatcta
121 gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt
181 ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata
241 acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga aattatatga
301 taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg
361 aacgatcggg gaaattcgga tccccaatac ttgtatgggg cttactaaaa gccagataac
421 agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc
481 gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga
541 cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc
601 atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg
661 atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc
721 tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt
781 ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag
841 tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga
901 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga
961 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
1021 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gacccataca
1081 agtattggag cgtaatctgg aacatcgtat gggtacatgg atcccccggg ctgcagaagt
1141 aacaccaaac aacagggtga gcatcgacaa aagaaacagt accaagcaaa taaatagcgt
1201 atgaaggcag ggctaaaaaa atccacatat agctgctgca tatgccatca tccaagtata
1261 tcaagatcaa aataattata aaacatactt gtttattata atagataggt actcaaggtt
1321 agagcatatg aatagatgct gcatatgcca tcatgtatat gcatcagtaa aacccacatc
1381 aacatgtata cctatcctag atcgatattt ccatccatct taaactcgta actatgaaga
1441 tgtatgacac acacatacag ttccaaaatt aataaataca ccaggtagtt tgaaacagta
1501 ttctactccg atctagaacg aatgaacgac cgcccaacca caccacatca tcacaaccaa
1561 gcgaacaaaa agcatctctg tatatgcatc agtaaaaccc gcatcaacat gtatacctat
1621 cctagatcga tatttccatc catcatcttc aattcgtaac tatgaatatg tatggcacac
1681 acatacagat ccaaaattaa taaatccacc aggtagtttg aaacagaatt ctactccgat
1741 ctagaacgac cgcccaacca gaccacatca tcacaaccaa gacaaaaaaa agcatgaaaa
1801 gatgacccga caaacaagtg cacggcatat attgaaataa aggaaaaggg caaaccaaac
1861 cctatgcaac gaaacaaaaa aaatcatgaa atcgatcccg tctgcggaac ggctagagcc
1921 atcccaggat tccccaaaga gaaacactgg caagttagca atcagaacgt gtctgacgta
1981 caggtcgcat ccgtgtacga acgctagcag cacggatcta acacaaacac ggatctaaca
2041 caaacatgaa cagaagtaga actaccgggc cctaaccatg gaccggaacg ccgatctaga
2101 gaaggtagag aggggggggg ggggaggacg agcggcgtac cttgaagcgg aggtgccgac
2161 gggtggattt gggggagatc tggttgtgtg tgtgtgcgct ccgaacaaca cgaggttggg
2221 gaaagagggt gtggaggggg tgtctattta ttacggcggg cgaggaaggg aaagcgaagg
2281 agcggtggga aaggaatccc ccgtagctgc cgtgccgtga gaggaggagg aggccgcctg
2341 ccgtgccggc tcacgtctgc cgctccgcca cgcatttctg gatgccgaca gcggagcaag
2401 tccaacggtg gagcggaact ctcgagaggg gtccagaggc agcgacagag atgccgtgcc
2461 gtctgcttcg cttggcccga cgcgacgctg ctggttcgct ggttggtgtc cgttagactc
2521 gtcgacggcg tttaacaggc tggcattatc tactcgaaac aagaaaaatg tttccttagt
2581 ttttttaatt tcttaaaggg tatttgttta atttttagtc actttatttt attctatttt
2641 atatctaaat tattaaataa aaaaactaaa atagagtttt agttttctta atttagaggc
2701 taaaatagaa taaaatagat gtactaaaaa aattagtcta taaaaaccat taaccctaaa
2761 ccctaaatgg atgtactaat aaaatggatg aagtattata taggtgaagc tatttgcaaa
2821 aaaaaaggag aacacatgca cactaaaaag ataaaactgt agagtcctgt tgtcaaaata
2881 ctcaattgtc ctttagacca tgtctaactg ttcatttata tgattctcta aaacactgat
2941 attattgtag tactatagat tatattattc gtagagtaaa gtttaaatat atgtataaag
3001 atagataaac tgcacttcaa acaagtgtga caaaaaaaat atgtggtaat tttttataac
3061 ttagacatgc aatgctcatt atctctagag aggggcacga ccgggtcacg ctgcactgca
3121 ggaattcgat atcaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc
3181 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccag
//
*Caution
Product is for research use only!
Search Name
pCXUN-HA, pCXUN-HA vector, pCXUN-HA plasmid, pCXUN-HA map, pCXUN-HA sequence