pDC316
Catalog No. PVT11041
Packing 2ug
pDC316 Informaiton
pDC316 Sequence
LOCUS Exported 3914 bp ds-DNA circular SYN 24-SEP-2017
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3914)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 24, 2017
FEATURES Location/Qualifiers
source 1..3914
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature complement(11..102)
/label=ITR
misc_signal 190..340
/label=Ad5 Psi
/note="packaging signal for adenovirus serotype 5"
misc_feature 469..993
/label=MCMV promoter
misc_feature 994..1029
/label=MCS
polyA_signal 1037..1171
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
protein_bind 1191..1224
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
rep_origin complement(1648..2236)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2407..3267)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3268..3372)
/gene="bla"
/label=AmpR promoter
misc_feature 3455..453
/label=Ad
misc_feature 3824..1
/label=ITR
ORIGIN
1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt
61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg
121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg
181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt
241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa
301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg
361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc
421 gggtcaaagt tggcgtttta ttattatagt cagctctaga gatatactga gtcattaggg
481 actttccaat gggttttgcc cagtacataa ggtcaatagg ggtgaatcaa caggaaagtc
541 ccattggagc caagtacact gagtcaatag ggactttcca ttgggttttg cccagtacaa
601 aaggtcaata gggggtgagt caatgggttt ttcccattat tggcacgtac ataaggtcaa
661 taggggtgag tcattgggtt tttccagcca atttaattaa aacgccatgt actttcccac
721 cattgacgtc aatgggctat tgaaactaat gcaacgtgac ctttaaacgg tactttccca
781 tagctgatta atgggaaagt accgttctcg agccaataca cgtcaatggg aagtgaaagg
841 gcagccaaaa cgtaacaccg ccccggtttt cccctggaaa ttccatattg gcacgcattc
901 tattggctga gctgcgttct acgtgggtat aagaggcgcg accagcgtcg gtaccgtcgc
961 agtcttcggt ctgaccaccg tagaacgcag atcgaattcc ccgggagatc taagcttgag
1021 ctcgtcgact tcgagcaact tgtttattgc agcttataat ggttacaaat aaagcaatag
1081 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
1141 actcatcaat gtatcttatc atgtctggat cgtctagcat cgaagatcca ataacttcgt
1201 atagcataca ttatacgaag ttataagtag cttggcgtaa tcatggtcat agctgtttcc
1261 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
1321 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
1381 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
1441 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
1501 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
1561 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
1621 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
1681 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
1741 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
1801 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta
1861 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
1921 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
1981 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
2041 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
2101 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
2161 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
2221 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
2281 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
2341 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
2401 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
2461 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
2521 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
2581 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
2641 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
2701 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
2761 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
2821 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
2881 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
2941 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
3001 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa
3061 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
3121 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
3181 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaa
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