pDC316-mCMV-EGFP
Catalog No. PVT11042
Packing 2ug
pDC316-mCMV-EGFP Information
Alias: pDC316-mCMV-EGFP-CMV-MCS
Prokaryotic resistance: Aampicillin(Amp)
Screening markers: green fluorescent protein EGFP
Cloned strain: Escherichia coli Stbl3
Culture conditions: 37℃
pDC316-mCMV-EGFP Description
PDC316-mCMV-EGFP is an adenovirus Admax expression plasmid.
pDC316-mCMV-EGFP Sequence
LOCUS Exported 5879 bp ds-DNA circular SYN 10-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..5879
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 468..992
/label=mCMV promoter
CDS 999..1715
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
polyA_signal 1862..1983
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
enhancer 1991..2370
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2371..2574
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 2619..2637
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 2651..2740
/label=MCS
polyA_signal 2764..2988
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
polyA_signal 3002..3136
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
protein_bind 3156..3189
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
rep_origin complement(3613..4201)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4372..5232)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5233..5337)
/gene="bla"
/label=AmpR promoter
misc_feature 5420..453
/label=Ad
ORIGIN
1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt
61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg
121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg
181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt
241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa
301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg
361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc
421 gggtcaaagt tggcgtttta ttattatagt cagtctagag atatactgag tcattaggga
481 ctttccaatg ggttttgccc agtacataag gtcaataggg gtgaatcaac aggaaagtcc
541 cattggagcc aagtacactg agtcaatagg gactttccat tgggttttgc ccagtacaaa
601 aggtcaatag ggggtgagtc aatgggtttt tcccattatt ggcacgtaca taaggtcaat
661 aggggtgagt cattgggttt ttccagccaa tttaattaaa acgccatgta ctttcccacc
721 attgacgtca atgggctatt gaaactaatg caacgtgacc tttaaacggt actttcccat
781 agctgattaa tgggaaagta ccgttctcga gccaatacac gtcaatggga agtgaaaggg
841 cagccaaaac gtaacaccgc cccggttttc ccctggaaat tccatattgg cacgcattct
901 attggctgag ctgcgttcta cgtgggtata agaggcgcga ccagcgtcgg taccgtcgca
961 gtcttcggtc tgaccaccgt agaacgcaga tcgaattcat ggtgagcaag ggcgaggagc
1021 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
1081 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
1141 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
1201 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
1261 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
1321 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
1381 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
1441 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
1501 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
1561 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
1621 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
1681 ccgggatcac tctcggcatg gacgagctgt acaagtccgg actcagatct cgagatccac
1741 cggatctaga taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt
1801 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
1861 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
1921 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
1981 ttaacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
2041 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
2101 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
2161 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
2221 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
2281 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
2341 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg</
....
//
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.
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