pDM4
PVT6015 2ug
pDM4 information
Replicon: R6K
Plasmid classification: large intestine series plasmids; large intestine editing plasmids; large intestine sacB plasmids
Plasmid size: 7104 bp
Prokaryotic resistance: Chl
Eukaryotic resistance: sacB
Clone strain: s17-1 λ pir
Culture conditions: 37℃
5 'sequencing primer: cat-r (ggtatttattcgggcgaaagtggc)
3 'sequencing primer: cat-f (gtgacacaatcacgaaacgcgg)
Plasmid host: Escherichia coli
Plasmid use: gene editing
Fragment type: ORF
Fragment species: empty vector
Prokaryotic resistance: Chl
pDM4 Description
pDM4 plasmid is a suicidal vector, which needs the strain containing PRI gene. The replicon is R6K γ ori, orit, and the plasmid size is 7104bp. It is chloramphenicol resistance. The culture conditions are lb, 37 ℃, aerobic.
pDM4 Plasmid Sequence
LOCUS Exported 7104 bp ds-DNA circular SYN 27-JUN-2017
DEFINITION synthetic circular DNA
KEYWORDS pDM4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7104)
TITLE Direct Submission
JOURNAL Exported Tuesday, June 27, 2017
FEATURES Location/Qualifiers
source 1..7104
/organism="synthetic DNA construct"
/mol_type="other DNA"
oriT 623..732
/note="incP origin of transfer"
CDS 765..1136
/codon_start=1
/gene="traJ"
/product="oriT-recognizing protein"
/note="traJ"
/translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
KQDELGKVMMGVVRPRAEP"
rep_origin 1754..2142
/note="R6K gamma ori"
/note="gamma replication origin from E. coli plasmid R6K;
requires the R6K initiator protein pi for replication"
protein_bind 2177..2193
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
misc_feature 2204..2313
/note="MCS"
promoter 2574..2676
/note="cat promoter"
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
CDS 2677..3336
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
promoter 3753..4198
/gene="sacR"
/note="sacB promoter"
/note="sacB promoter and control region"
CDS 4199..5620
/codon_start=1
/gene="Bacillus subtilis sacB"
/product="secreted levansucrase that renders bacterial
growth sensitive to sucrose"
/note="SacB"
/note="negative selection marker"
/translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
VKDSILEQGQLTVNK"
ORIGIN
1 gatccttttt gtccggtgtt gggttgaagg tgaagccggt cggggccgca gcgggggccg
61 gcttttcagc cttgcccccc tgcttcggcc gccgtggctc cggcgtcttg ggtgccggcg
121 cgggttccgc agccttggcc tgcggtgcgg gcacatcggc gggcttggcc ttgatgtgcc
181 gcctggcgtg cgagcggaac gtctcgtagg agaacttgac cttccccgtt tcccgcatgt
241 gctcccaaat ggtgacgagc gcatagccgg acgctaacgc cgcctcgaca tccgccctca
301 ccgccaggaa cgcaaccgca gcctcatcac gccggcgctt cttggccgcg cgggattcaa
361 cccactcggc cagctcgtcg gtgtagctct ttggcatcgt ctctcgcctg tcccctcagt
421 tcagtaattt cctgcatttg cctgtttcca gtcggtagat attccacaaa acagcaggga
481 agcagcgctt ttccgctgca taaccctgct tcggggtcat tatagcgatt ttttcggtat
541 atccatcctt tttcgcacga tatacaggat tttgccaaag ggttcgtgta gactttcctt
601 ggtgtatcca acggcgtcag ccgggcagga taggtgaagt aggcccaccc gcgagcgggt
661 gttccttctt cactgtccct tattcgcacc tggcggtgct caacgggaat cctgctctgc
721 gaggctggcc ggctaccgcc ggcgtaacag atgagggcaa gcggatggct gatgaaacca
781 agccaaccag gaagggcagc ccacctatca aggtgtactg ccttccagac gaacgaagag
841 cgattgagga aaaggcggcg gcggccggca tgagcctgtc ggcctacctg ctggccgtcg
901 gccagggcta caaaatcacg ggcgtcgtgg actatgagca cgtccgcgag ctggcccgca
961 tcaatggcga cctgggccgc ctgggcggcc tgctgaaact ctggctcacc gacgacccgc
1021 gcacggcgcg gttcggtgat gccacgatcc tcgccctgct ggcgaagatc gaagagaagc
1081 aggacgagct tggcaaggtc atgatgggcg tggtccgccc gagggcagag ccatgacttt
1141 tttagccgct aaaacggccg gggggtgcgc gtgattgcca agcacgtccc catgcgctcc
1201 atcaagaaga gcgacttcgc ggagctggtg aagtacatca ccgacgagca aggcaagacc
1261 gagcgcctgg gtcacgtgcg cgtcacgaac tgcgaggcaa acaccctgcc cgctgtcatg
1321 gccgaggtga tggcgaccca gcacggcaac acccgttccg aggccgacaa gacctatcac
1381 ctgctggtta gcttccgcgc gggagagaag cccgacgcgg agacgttgcg cgcgattgag
1441 gaccgcatct gcgctgggct tggcttcgcc gagcatcagc gcgtcagtgc cgtgcatcac
1501 gacaccgaca acctgcacat ccatatcgcc atcaacaaga ttcacccgac ccgaaacacc
1561 atccatgagc cgtatcgggc ctaccgcgcc ctcgctgacc tctgcgcgac gctcgaacgg
1621 gactacgggc ttgagcgtga caatcacgaa acgcggcagc gcgtttccga gaaccgcgcg
1681 aacgacatgg agcggcacgc gggcgtggaa agcctggtcg gctggatccg gccacgatgc
1741 gtccggcgta gaggatctga agatcagcag ttcaacctgt tgatagtacg tactaagctc
1801 tcatgtttca cgtactaagc tctcatgttt aacgtactaa gctctcatgt ttaacgaact
1861 aaaccctcat ggctaacgta ctaagctctc atggctaacg tactaagctc tcatgtttca
1921 cgtactaagc tctcatgttt gaacaataaa attaatataa atcagcaact taaatagcct
1981 ctaaggtttt aagttttata agaaaaaaaa gaatatataa ggcttttaaa gcttttaagg
2041 tttaacggtt gtggacaaca agccagggat gtaacgcact gagaagccct tagagcctct
2101 caaagcaatt ttgagtgaca caggaacact taacggctga catgggaatt ccacatgtgg
2161 aattccacat gtggaattgt gagcggataa caatttgtgg aatcccggga gagctcaggt
2221 tacccgcatg caagatctat ctagaagggc cccactagtg acgcgtactc gagggtcgac
2281 ggtatcgata agcttgatat acactccgct agcgctgatg tccggcggtg cttttgccgt
2341 tacgcaccac cccgtcagta gctgaacagg agggacagct gatagaaaca gaagccactg
2401 gagcacctca aaaacaccat catacactaa atcagtaagt tggcagcatc acccgacgca
2461 ctttgcgccg aataaatacc tgtgacggaa gatcacttcg cagaataaat aaatcctggt
2521 gtccctgttg ataccgggaa gccctgggcc aacttttggc gaaaatgaga cgttgatcgg
2581 cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt
2641 gagttatcga gattttcagg agctaargaa gctaaaatgg agaaaaaaat cactggatat
2701 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
2761 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
2821 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
2881 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
2941 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
3001 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
3061 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
3121 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
3181 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
3241 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
3301 caacagtact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag ttattggtgc
3361 ccttaaacgc ctggttgcta cgcctgaata agtgataata agcggatgaa tggcagaaat
3421 tcgaaagcaa attcgacccg gtcgtcggtt cagggcaggg tcgttaaata gccgcttatg
3481 tctattgctg gtttaccggt ttattgacta ccggaagcag tgtgaccgtg tgcttctcaa
3541 atgcctgagg ccagwttgct cagctctccc gtggaggtaa taattgacga tatgatcatt
3601 tattctgcct cccagagcct gataaaaacg gttagcgctt cgttaataca gatgtaggtg
3661 ttccacaggg tagccagcag catcctgcga tgcagatcat cgaattcctg cagccaagct
3721 agacctaggc cttaagatcc tttttaaccc atcacatata cctgccgttc actattattt
3781 agtgaaatga gatattatga tattttctga attgtgatta aaaaggcaac tttatgccca
3841 tgcaacagaa actataaaaa atacagagaa tgaaaagaaa cagatagatt ttttagttct
3901 ttaggcccgt agtctgcaaa tccttttatg attttctatc aaacaaaaga ggaaaataga
3961 ccagttgcaa tccaaacgag agtctaatag aatgaggtcg aaaagtaaat cgcgcgggtt
4021 tgttactgat aaagcaggca agacctaaaa tgtgtaaagg gcaaagtgta tactttggcg
4081 tcacccctta catattttag gtcttttttt attgtgcgta actaacttgc catcttcaaa
4141 caggagggct ggaagaagca gaccgctaac acagtacata aaaaaggaga catgaacgat
4201 gaacatcaaa aagtttgcaa aacaagcaac agtattaacc tttactaccg cactgctggc
4261 aggaggcgca actcaagcgt ttgcgaaaga aacgaaccaa aagccatata aggaaacata
4321 cggcatttcc catattacac gccatgatat gctgcaaatc cctgaacagc aaaaaaatga
4381 aaaatatcaa gttcctgaat tcgattcgtc cacaattaaa aatatctctt ctgcaaaagg
4441 cctggacgtt tgggacagct ggccattaca aaacgctgac ggcactgtcg caaactatca
4501 cggctaccac atcgtctttg cattagccgg agatcctaaa aatgcggatg acacatcgat
4561 ttacatgttc tatcaaaaag tcggcgaaac ttctattgac agctggaaaa acgctggccg
4621 cgtctttaaa gacagcgaca aattcgatgc aaatgattct atcctaaaag accaaacaca
4681 agaatggtca ggttcagcca catttacatc tgacggaaaa atccgtttat tctacactga
4741 tttctccggt aaacattacg gcaaacaaac actgacaact gcacaagtta acgtatcagc
4801 atcagacagc tctttgaaca tcaacggtgt agaggattat aaatcaatct ttgacggtga
4861 cggaaaaacg tatcaaaatg tacagcagtt catcgatgaa ggcaactaca gctcaggcga
4921 caaccatacg ctgagagatc ctcactacgt agaagataaa ggccacaaat acttagtatt
4981 tgaagcaaac actggaactg aagatggcta ccaaggcgaa gaatctttat ttaacaaagc
5041 atactatggc aaaagcacat cattcttccg tcaagaaagt caaaaacttc tgcaaagcga
5101 taaaaaacgc acggctgagt tagcaaacgg cgctctcggt atgattgagc taaacgatga
5161 ttacacactg aaaaaagtga tgaaaccgct gattgcatct aacacagtaa cagatgaaat
5221 tgaacgcgcg aacgtcttta aaatgaacgg caaatggtac ctgttcactg actcccgcgg
5281 atcaaaaatg acgattgacg gcattacgtc taacgatatt tacatgcttg gttatgtttc
5341 taattcttta actggcccat acaagccgct gaacaaaact ggccttgtgt taaaaatgga
5401 tcttgatcct aacgatgtaa cctttactta ctcacacttc gctgtacctc aagcgaaagg
5461 aaacaatgtc gtgattacaa gctatatgac aaacagagga ttctacgcag acaaacaatc
5521 aacgtttgcg ccaagcttcc tgctgaacat caaaggcaag aaaacatctg ttgtcaaaga
5581 cagcatcctt gaacaaggac aattaacagt taacaaataa aaacgcaaaa gaaaatgccg
5641 atatcctatt ggcattttct tttatttctt atcaacataa aggtgaatcc catatgaact
5701 atataaaagc aggcaaatgg ctaaccgtat tcctaacctt ttggtaatga ctccaactta
5761 ttgatagtgt tttatgttca gataatgccc gatgactttg tcatgcagct ccaccgattt
5821 tgagaacgac agcgacttcc gtcccagccg tgccaggtgc tgcctcagat tcaggttatg
5881 ccgctcaatt cgctgcgtat atcgcttgct gattacgtgc agctttccct tcaggcggga
5941 ttcatacagc ggccagccat ccgtcatcca tatcaccacg tcaaagggtg acagcaggct
6001 cataagacgc cccagcgtcg ccatagtgcg ttcaccgaat acgtgcgcaa caaccgtctt
6061 ccggagactg tcatacgcgt aaaacagcca gcgctggcgc gatttagccc cgacatagcc
6121 ccactgttcg tccatttccg cgcagacgat gacgtcactg cccggctgta tgcgcgaggt
6181 taccgactgc ggcctgagtt ttttaagtga cgtaaaatcg tgttgaggcc aacgcccata
6241 atgcgggctg ttgcccggca tccaacgcca ttcatggcca tatcaatgat tttctggtgc
6301 gtaccgggtt gagaagcggt gtaagtgaac tgcagcaatg gcaacaacgt tgcgcaaact
6361 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
6421 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
6481 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
6541 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
6601 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
6661 agtttactca tatatacttt agattgattt atggtgcact ctcagtacaa tctgctctga
6721 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
6781 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
6841 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
6901 tcaccgaaac gcgcgaggca gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
6961 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
7021 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
7081 ggccacgatg cgtccggcgt agag
//
Caution
Product is for research use only!
Search name
pDM4,Plasmid pDM4,pDM4 vector
