LOCUS Exported 4757 bp ds-DNA circular SYN 18-10-2015
KEYWORDS Untitled 6
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4757)
AUTHORS admin
TITLE Direct Submission
JOURNAL Exported 2015-10-18
FEATURES Location/Qualifiers
source 1..4757
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 663..1337
/codon_start=1
/product="improved tetrameric variant of DsRed fluorescent
protein"
/note="DsRed2"
/note="mammalian codon-optimized"
/translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
LFL"
misc_feature 1365..1421
/note="MCS"
/note="multiple cloning site"
polyA_signal 1545..1666
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1673..2128)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2155..2259
/gene="bla"
/note="AmpR promoter"
promoter 2261..2618
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2469..2604
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2653..3447
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3679..3726
/note="HSV TK poly(A) signal"
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 4055..4643
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgc
601 tgctatccgt gccgttgctg ctcggcctcc tcggcctggc cgtcgccgac cggtcgcaca
661 ccatggcctc ctccgagaac gtcatcaccg agttcatgcg cttcaaggtg cgcatggagg
721 gcaccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
781 gccacaacac cgtgaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca
841 tcctgtcccc ccagttccag tacggctcca aggtgtacgt gaagcacccc gccgacatcc
901 ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
961 aggacggcgg cgtggcgacc gtgacccagg actcctccct gcaggacggc tgcttcatct
1021 acaaggtgaa gttcatcggc gtgaacttcc cctccgacgg ccccgtgatg cagaagaaga
1081 ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg
1141 agacccacaa ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagtcca
1201 tctacatggc caagaagccc gtgcagctgc ccggctacta ctacgtggac gccaagctgg
1261 acatcacctc ccacaacgag gactacacca tcgtggagca gtacgagcgc accgagggcc
1321 gccaccacct gttcctgaga tcgtacaaga aggacgagct gtaaagatct cgagctcaag
1381 cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggatct agataactga
1441 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
1501 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
1561 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
1621 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta
1681 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
1741 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
1801 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
1861 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
1921 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
1981 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
2041 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
2101 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga
2161 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
2221 ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc
2281 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
2341 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
2401 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
2461 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
2521 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
2581 agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga
2641 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
2701 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
2761 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
2821 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
2881 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
2941 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
3001 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
3061 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
3121 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
3181 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
3241 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
3301 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
3361 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
3421 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
3481 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
3541 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
3601 tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg
3661 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt
3721 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
3781 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
3841 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt
3901 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
3961 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
4021 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
4081 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
4141 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
4201 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtatca ccgcctacat
4261 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
4321 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
4381 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
4441 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
4501 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
4561 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
4621 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
4681 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
4741 gtattaccgc catgcat
//
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.
Search name
pDsRed2-ER,Plasmid pDsRed2-ER,pDsRed2-ER vector
English