pECMV-Scn10a-m-FLAG Plasmid

PVT15248   2ug
 

pECMV-Scn10a-m-FLAG Plasmid Information

Promoter CMV

Prokaryotic resistance Kan

Screening marker G418

Clone strain DH5a

Culture Conditions LB/37 Celsius degree

Forward primer CMV-F

Reverse primer pFLAG-CMV-R

pECMV-Scn10a-m-FLAG Plasmid Description

pECMV-Scn10a-m-FLAG Plasmid is a ORF expression plasmid

pECMV-Scn10a-m-FLAG Plasmid Sequence

TGCACTTCAGGGGCGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCG
CGGCCGGCCGTTTAAACCTTATCGTCGTCATCCTTGTAATCCAGGATATCATTTGCTGCCAGATCCTCTTCTGAGATGAG
TTTCTGCTCGAGCGGCCGCGTACGCGTCGGTCCGCTCTGAGGTCCAGGGCTCTTCCCTTCCTTAGCAGTGACTTCATCTT
CATTTTGCATTGAGCTAGATGTGCTAATGTTGGCCCTGTCACTCAGGCCCCTGGTGACGCTGTCATAGGATGGTGGGAAA
GACGTAGCAGAAGCAGTTTCCGATTTGTCTGGGAGCCCACCGTTGTCATTTGCCATGAATGTAACATAGCCTTCCCTGGG
GAGTGACACGCCATCCTCCTCAGCCCTGGGCACATGCAGGGGGTTGGAGAGCATCAAGGAGCGTTGCAACATGTAGTTCC
GATAGGCCTTTTGAATAATGGTGGCTGAGATGTCTTCCTGCTTGCACCGGAGGGTGGTTGCTATTGGTTCATAGGATGCT
TTGGAAAGATTAGTTGCCATAAACTTCTCTTCCATATTAGTCTTCAGAGAATCCAACTCCCCAGATTCTCCCAAGACATT
CTTTGTGAAGGCAAAGAGGATGTCCAAACAGTGGATCTTATCTCCGGGGACCAACGGCAGGTCCATCTGGATTAATATAT
TCTGATTAGGTTTTGGGATTCTAAGAGGGCCAGAGAGTGTGTCTGCAAAGTCTGAGAGGGCAGAAAAGGCAATGAACTGG
GTGGCCTCCGGGTCAAACTTCTCCCAGGTCTCATAGAACATGTCAAAGTCGTCCTCGCTCAGGGGCTCCGTGCTCTCTTC
TGTGGCCACATTGAAGTTCTCCAGAATCACTGCAATGTACATGTTGACCACGATGAGGAAGGAGATGATGATGTAGGTGG
TGAAGAAGAGGATGCCCACCGCTGGGCTTCCACAATTCCCCTTGGAGCCATTGCTGTTGGGCCGGTTGGGGTCGCAGTAG
GGGGGTCCTGTGTTGAGGATGGGGCTGAGGAGGCCATCCCAGCCAGCCGACGTGGTGATCTGGAAAAGGCACAGCATGCT
GTTGCCAAAGGTCTTGAAGTTGAACATGTCGTCGATGCCAGCCTCATCTATGACATTAGCGAAGCTGGCCATGCCGAAGA
TGGAGTAGATGAACATGACGAGGAAGAGGAGGAGGCCGATGTTGAAGAGGGCGGGCAGGGACATCATGAGGGCGAAGAGC
AGCGTGCGAATCCCCTTGGCTGCTCGAATCAGCCTGAGGATGCGGCCGATCCTGGCCAGACGGATGACGCGTAAGAGCGT
GGGGGAGAAGTAACTTTCTAGTGAGCTAAGGATCGCAGAAAACAACAGACTAGAAATGGACAGAATCACCACAATGAAGT
CGAACACATTCCAGCCGTTGGTGAAGTAATACTGCCGAAGGGCGAACATCTTCATCACACACTCGCCCGTGAAGACGGCC
ACGAAGAACTGGTTGATTCTGCCCAGGACCTTCGTCTTCTCCTCGCTCTGATTGTCGGTCTCCACCATCATGGTGATCAT
GTTGAGGCAGATGAGAGCCATGATGATGATGTCAAATGCTTGCCTGGTCACAATGTCAAACACGAAGCCCTGGTACTTAT
TCAAAGGCCGTGGGATGGGCTTCTGGGGTTTCTTGGAGCCCAGCTTCTTCATGGCATTGTAGTACTTCTTCTGCTCCTCT
GTCATGAAGATGTCCTGGCCCCCTAGCTTTTTTTTCTGTTGATTGAAGTTGTCAATGATGACCCCGACAAAGAGATTCAG
CGTGAAGAAGCCACCGAAAATGATGAAGACGACGAAGTATAGGTACATGTACAGGCTCTCCTCCCAATTGGGCTGACTGT
TGATATCTCGAGAATCGACAGCTGCATACATAATGTCCATCCAGCCTTTGAAGGTTGCCACCTGGAGAAGCGCGAGGTAG
CCCATAGCAACGTTGTCGAAGTTGACTTTGACGTTAACCCAGAAGAAGTGGCCAGTATTGTTTTGATTGTAACAGTCAGA
CTTGTTAGTCACGAATGTCGAATTCACGACGGAAAATGGGTTGCTTCTGGTGTCGACACATCTCGAAAATTTCCCGGCGA
AGAGGTTCACACCCATGATGCTGAAGATGAGCCAGAAGATGAGGCAGACGAGGAGGACGTTCATGATGGAGGGAATGGCG
CCCACCAAGGCATCCACCACTACCCTCATGCCTTCGAATCGAGACAGAGCCCGCAGCGGCCGGAGGGCACGGAGAGTCCG
AAGGGCTTTGATGGACGCCACGTCTGAATACTCGAGGATCTTGGCTATGAGGCTTGTGAGGGAGATATTCACGATGAGGA
AGTCCAGCCAGCACCAGGCATTGGTGAAATATTTTTTGAAGCCATAAGCTACCCACTTGAGCAACATCTCGAATACAAAG
ATGAAGGTGAACACTCTGTCAGTGTACTCCAGCACAGACTTCACTCGGGGCTTTTCTTCCAGGTAGTTATCCTCAAAGGC
CAGCGCTCCACTGCTGAGCAGGATCATGAAGATAATGAAACTCTCAAACCAGCTGTGCTCCACAATGCGGTAACAGGTTT
TGCGCACCTGCCAGCCCGTGGCCCAAGGAAACTTACTGGTGTTCACTTTGCAGCAGGGACAGCGGCGAGTGCAGCCTTCT
GGGAAACAGTCATCGGGCTCGTCCAGCTCCTCCGCCAGCTCAGGGATCTTCCTCAGGATCTCCTCTGGGTCCGGGCAGTC
CACCGTGCTGCCCTCGGAGGAGCTTGTGTCATCCACTCCCTCGGCAGGGACCCGAGGGCTCTCCTCCCTCTGCCATCTCT
CCCCCAGGTATGGAGCCAGGTCCTCAGAGGACATCCCGGAGGGTGGGCTTCGGGCTGCCTGGTGATCTTCACACTTTTGG
ACTTGCTGCAGCAGCTCCTGCCCTTTGGGGCTCTCTTCCTGCCAGGAGCTCTGAGAAGCATGCTCCACATCTTCCTCGAG
CTCATCAAGGTCGGACTCCCCCTCAGCAATGGGCACAGAGACCCACACGTTAGGATCAGTGATGAAGTCCCCGTGGTTCT
CCTTGGGGCCACCAAGAGCTGGCTTTGCCAGGTTCCCCACTGCAGCATTGACAGCATCAGTAGCAATGTGGTTCTCAGCT
TTGCAGCTGGTGAGTGGGGGTTTCATCCCCAGCTGGGTCTCCACCTTGGGCCAGCGGAGCCGGCAGTGGCTTCTGATGTA
ACTGGTAATGGCCCGACTGGCCCGATGGCCAAATACCTGAATCCGGGCCAGTGCTACCTGCAAGTTGTTCACCTCCCCGT
CATCCTCTGGGGCTGTGAGGTTGTCCGCACTGAAGGAGTTCAGCAGTAAAGCGATGAATAGGTTGAGCACCACCAGGTTG
CCTAGCACCATCACTGTCAAGAAGAGGGTGAGGCAGATGTAGTCCTGGCTGACCTCCATGCAGACCCACATGTTCTCGAT
CCACTCCCCGCAGAGGATCCGGAAGACGACGAGGAAGGAATGGAAGAAGTCACACATGTGCCAGCGCAGCCTCTCACCAT
TCCACACGGAGATGCCATCCCTGCGGCACCCATAGTTCTCTGAGAGGAGCTGCTTTCCCACCAGGGCGAAGATAAAGACG
ATGATGGCCAGGATGAAGGTCAGGTTGCCCAGGGCCCCCACAGAGTTCCCGATGATCTTGATGAGCATGTTCAGGGTGGG
CCAGGACTTGGCCAGCTTGAAGACCCGAAGCAAGCGGAAGGTGCGGAGCACAGACAAGCTGCCCTTCTTGGATGTGCTCA
GCTCCAGCAGGCTCACGGTGACGATGACACAGTCGAAGATGTTCCACTTCTTCTGGAAGTAGTAGTAGGGGTCGAAGGCA
ATGATCTTGAAGGCCATCTCCATTGTAAAAAACACAGTAAAGACAATGTTGCCGGCTTGGAGCATGGCATCGAAGGCATC
AGTCATGGGGTAGTGTTCCATGGCCATGAAGACGGTATTCACCACAATGCACAGGGTGATGGTGAGCTCTGCGAAGGGGT
CAGTCACCAGCTCGAAGAGCACCATCTTGAATTTCTTCCACTTGGGGCAGCACTCCCATATCAGGTACTTCTGGGCTAAG
CTGATCAAGCAGGGTGGGCACTTCAGCTTAGACTCTTCCAGCTCCTCAATGACAGAAGTCATAATACTGACAACACTCAT
TGCCCTCTGTGCTCGGAAAGGTTCATTCAAGTAGTCTGCAGACAGGAAGTTCTTCTGTCCTGCAGCATCGAGTGCCGGGC
CTTCAGGCGCTCCAGTGGCAAGCTCACCAGTGGGCACTCCACGCTGTCCCTCTTCTCCACGTCTAGGGCCAGGGTTGGGG
GACTGAGGCAGTGGACTCCTGGGGAGAGGACCTGCCTGCCCGGCACCCCTGCCCAGCAATATGGAACTTCGACGGCTTTC
CTGATCTCCATGAAAGACCCCGTCATCCAAGATCCCATCAGGAAATGAGACGTCTTGGCTGGGTGCTCGGAAGTGGAACA
CACTGCTGTGGCTAGCCCTGCGTCTTCCAGAAGAAAGGCCTAGGAAAGACATCCTGCGCTGGTTGTAAGGGTCGGATTGT
AGTGATCTGTTGTCATCTGTCGAGCCTTCTGACATCCTTGATTTCACCCTGGGTCTTCTCTCATTGGCGTTTTTGGGGGC
TAAGGGTGATCCGTTGTGGGAATAGAGCGAGGTTGTGTCAATGCCCAGCGCTGCCAGCACCTCCTGTTCTTTCTGCAGCA
CCTCGAGGGCTTCCTTGAACTTCTTCTCCTTGGCTTCGATTTCTGCAATTGTTGCCTGGCTCTGTTCCTCATATGCCATG
GTGACCACAGCCAAGATCAAATTGACCAGGTAGAATGATCCAAGGAAGATGACCAGCACAAAAAAGACCATGTACATTTT
CCCGGAAGCCCGGAGTGTCTGCTGGTACAGCCGTTCCCAGGAGTCCTGCGTCATGAGACGGAACAGTGAGAGGAACGCCC
ACGCGAAGGAATCAAAGCTGGTGTAGTTAAAATCCGGGTTGTCAGAAGTTTTCCGGCAGACATAATCATTAGGGCAGTGG
CCAGCATCAGATCCATTGCCACACAACAAGGGATCCGTAGTACCGGGCTTGATGAAGATGTCTCCTGCCATTTCAGATGA
GAGATTGTCAGCCTTGTGCGGATCTGTGCCGTTCTTAATGCATTTATTCTTGAGGTTCCCCTTGAAGAGCTGCAGGCCCA
CCAAGGCAAAGACACTCAGGCAGAAGACTGTGAGGATGGTCACGTCGGCCAGCTTCCTCACTGAGTGGATCAGGGCTCCC
ACGATGACCTTCAGTCCTGGGATCACAGAAACAGTCTTCAGGGCCCTGAGAACTCGGAATGTCCGCAGGCCTGAGATTCC
TCGGAGGTCTATCGCTGCACCCACATACGCCAGGGTAATGACACTGAAGTCCAGCCAGTTCCAGGGATCTCGAAGATAAG
TGAATTCATTTAGACAAAACCCTCTTGCCAGTATCTTTATCAGAGCCTCGAAGGTGTAAACAACAGTGAAGGCATACTCG
AGTTTCTCTGGAAGATCAGTTCGGGTCATGCACACACAGTTGACCAAAATAGTGACAGTGATAAATATGGAGAACCAGGA
GTGGACGGACACTTTGATGGCTGTTCTTCTGATCAGGTTGAAGGGACTGAAGAGCCACAGAGCCCAAGTGGCACTGAATC
TGGAAATGGTCCTGCTTTTATCCAACACTATGAATGTCCGGTGTGTGCTGTAGAAAGGATCCAGGTCCTCCAGGGGCTCC
CCGACCAGCTCTGCTGGGAGCTCGCCATAGAACCTGGGCAGCTGGTTACAGGCCTTCAAGTCCAACTGAGGCCTGGGCTT
CTCACTCTTGTCCTTCTGTCCTCTTTGCTTAGGTCTGCCCTTCTTGGCGGCGCGGTGGGCAGCGATCTGCTTCTCGATCT
CTGCCAGCGACTCTGGAGTGAACCGTCTGAAGTTGGTAGTTCCCACGGACCCAAAGGGGAACTCCATGGCGATCGCGGCG
GCAGATCTCCTCGGTACCGGATCCAGTCGACGAATTCCCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTC
ACTAAACGAGCTCTCTATAGCTCCCACCC
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Caution:
1.  This product is FOR RESEARCH USE ONLY!
2.  The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.  Shipping temperature is 2-8℃.