LOCUS Exported 7569 bp ds-DNA circular SYN 11-JUL-2018
DEFINITION synthetic circular DNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7569)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..7569
/organism="synthetic DNA construct"
/mol_type="other DNA"
polyA_signal 116..237
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(999..1587)
/direction=LEFT
/label=pEE6 ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1758..2618)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2619..2723)
/gene="bla"
/label=AmpR promoter
terminator 2892..2919
/label=T7Te terminator
/note="phage T7 early transcription terminator"
promoter 3002..3331
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3182..3317
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3370..4491
/codon_start=1
/label=GS
/translation="MATSASSHLNKNIKQMYLCLPQGEKVQAMYIWVDGTGEGLRCKTR
TLDCEPKCVEELPEWNFDGSSTFQSEGSNSDMYLSPVAMFRDPFRRDPNKLVFCEVFKY
NRKPAETNLRHSCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYYC
GVGADKAYGRDIVEAHYRACLYAGVKITGTNAEVMPAQWEFQIGPCEGIRMGDHLWVAR
FILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKHIEEAIEKLSKRH
RYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASIRIPRTVGQEKKGYFEDR
RPSANCDPFAVTEAIVRTCLLNETGDEPFQYKN"
intron 4623..4688
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 4818..4838
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 5263..5397
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
enhancer 5966..6345
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 6346..6549
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature join(7529..7569,1..6)
/label=MCS
BASE COUNT 1904 a 1869 c 1805 g 1990 t
ORIGIN
1 gaattcattg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
61 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
121 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
181 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
241 tgtctggcgg ccgcgacctg caggcgcaga actggtaggt atggaagatc cctcgagatc
301 cattgtgctg gcggtaggcg agcagcgcct gcctgaagct gcgggcattc ccgatcagaa
361 atgagcgcca gtcgtcgtcg gctctcggca ccgaatgcgt atgattctcc gccagcatgg
421 cttcggccag tgcgtcgagc agcgcccgct tgttcctgaa gtgccagtaa agcgccggct
481 gctgaacccc caaccgttcc gccagtttgc gtgtcgtcag accgtctacg ccgacctcgt
541 tcaacaggtc cagggcggca cggatcactg tattcggctg caactttgtc atgcttgaca
601 ctttatcact gataaacata atatgtccac caacttatca gtgataaaga atccgcgcca
661 gcacaatgga tctcgaggtc gagggatctc tagaggatcc tctacgccgg acgcatcgtg
721 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
781 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
841 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
901 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
961 aagggagagc gtcgacctcg ggccgcgttg ctggcgtttt tccataggct ccgcccccct
1021 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
1081 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
1141 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
1201 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
1261 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
1321 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
1381 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
1441 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
1501 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
1561 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
1621 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
1681 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
1741 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
1801 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
1861 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
1921 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
1981 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
2041 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
2101 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
2161 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
2221 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
2281 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
2341 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
2401 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
2461 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
2521 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
2581 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
2641 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
2701 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
2761 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctgatggctc
2821 tttgcggcac ccatcgttcg taatgttccg tggcaccgag gacaaccctc aagagaaaat
2881 gtaatcacac tggctcacct tcgggtgggc ctttctgcgt ttataaggag acactttatg
2941 tttaagaagg ttggtaaatt ccttgcggct ttggcagcca agctagatcc ggctgtggaa
3001 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
3061 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
3121 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
3181 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
3241 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
3301 aggctttttt ggaggcctag gcttttgcaa aaagctagct tggggccacc gctcagagca
3361 ccttccacca tggccacctc agcaagttcc cacttgaaca aaaacatcaa gcaaatgtac
3421 ttgtgcctgc cccagggtga gaaagtccaa gccatgtata tctgggttga tggtactgga
3481 gaaggactgc gctgcaaaac ccgcaccctg gactgtgagc ccaagtgtgt agaagagtta
3541 cctgagtgga attttgatgg ctctagtacc tttcagtctg agggctccaa cagtgacatg
3601 tatctcagcc ctgttgccat gtttcgggac cccttccgca gagatcccaa caagctggtg
3661 ttctgtgaag ttttcaagta caaccggaag cctgcagaga ccaatttaag gcactcgtgt
3721 aaacggataa tggacatggt gagcaaccag cacccctggt ttggaatgga acaggagtat
3781 actctgatgg gaacagatgg gcaccctttt ggttggcctt ccaatggctt tcctgggccc
3841 caaggtccgt attactgtgg tgtgggcgca gacaaagcct atggcaggga tatcgtggag
3901 gctcactacc gcgcctgctt gtatgctggg gtcaagatta caggaacaaa tgctgaggtc
3961 atgcctgccc agtgggagtt ccaaatagga ccctgtgaag gaatccgcat gggagatcat
4021 ctctgggtgg cccgtttcat cttgcatcga gtatgtgaag actttggggt aatagcaacc
4081 tttgacccca agcccattcc tgggaactgg aatggtgcag gctgccatac caactttagc
4141 accaaggcca tgcgggagga gaatggtctg aagcacatcg aggaggccat cgagaaacta
4201 agcaagcggc accggtacca cattcgagcc tacgatccca aggggggcct ggacaatgcc
4261 cgtcgtctga ctgggttcca cgaaacgtcc aacatcaacg acttttctgc tggtgtcgcc
4321 aatcgcagtg ccagcatccg cattccccgg actgtcggcc aggagaagaa aggttacttt
4381 gaagaccgcc gcccctctgc caattgtgac ccctttgcag tgacagaagc catcgtccgc
4441 acatgccttc tcaatgagac tggcgacgag cccttccaat acaaaaacta attagacttt
4501 gagtgatctt gagcctttcc tagttcatcc caccccgccc cagagagatc tttgtgaagg
4561 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
4621 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
4681 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
4741 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
4801 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
4861 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
4921 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
4981 ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt tttcttactc
5041 cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct
5101 ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc
5161 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
5221 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
5281 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
5341 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctag
5401 cttcgtgtca aggacggtga ctgcagtgaa taataaaatg tgtgtttgtc cgaaatacgc
5461 gttttgagat ttctgtcgcc gactaaattc atgtcgcgcg atagtggtgt ttatcgccga
5521 tagagatggc gatattggaa aaatcgatat ttgaaaatat ggcatattga aaatgtcgcc
5581 gatgtgagtt tctgtgtaac tgatatcgcc atttttccaa aagtgatttt tgggcatacg
5641 cgatatctgg cgatagcgct tatatcgttt acgggggatg gcgatagacg actttggtga
5701 cttgggcgat tctgtgtgtc gcaaatatcg cagtttcgat ataggtgaca gacgatatga
5761 ggctatatcg ccgatagagg cgacatcaag ctggcacatg gccaatgcat atcgatctat
5821 acattgaatc aatattggcc attagccata ttattcattg gttatatagc ataaatcaat
5881 attggctatt ggccattgca tacgttgtat ccatatcata atatgtacat ttatattggc
5941 tcatgtccaa cattaccgcc atgttgacat tgattattga ctagttatta atagtaatca
6001 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
6061 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
6121 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
6181 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
6241 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
6301 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
6361 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
6421 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
6481 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
6541 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
6601 ctccatagaa gacaccggga ccgatccagc ctccgcggcc gggaacggtg cattggaacg
6661 cggattcccc gtgccaagag tgacgtaagt accgcctata gagtctatag gcccaccccc
6721 ttggcttctt atgcatgcta tactgttttt ggcttggggt ctatacaccc ccgcttcctc
6781 atgttatagg tgatggtata gcttagccta taggtgtggg ttattgacca ttattgacca
6841 ctcccctatt ggtgacgata ctttccatta ctaatccata acatggctct ttgccacaac
6901 tctctttatt ggctatatgc caatacactg tccttcagag actgacacgg actctgtatt
6961 tttacaggat ggggtctcat ttattattta caaattcaca tatacaacac caccgtcccc
7021 agtgcccgca gtttttatta aacataacgt gggatctcca cgcgaatctc gggtacgtgt
7081 tccggacatg ggctcttctc cggtagcggc ggagcttcta catccgagcc ctgctcccat
7141 gcctccagcg actcatggtc gctcggcagc tccttgctcc taacagtgga ggccagactt
7201 aggcacagca cgatgcccac caccaccagt gtgccgcaca aggccgtggc ggtagggtat
7261 gtgtctgaaa atgagctcgg ggagcgggct tgcaccgctg acgcatttgg aagacttaag
7321 gcagcggcag aagaagatgc aggcagctga gttgttgtgt tctgataaga gtcagaggta
7381 actcccgttg cggtgctgtt aacggtggag ggcagtgtag tctgagcagt actcgttgct
7441 gccgcgcgcg ccaccagaca taatagctga cagactaaca gactgttcct ttccatgggt
7501 cttttctgca gtcaccgtcc ttgacacgaa gcttcgcgac gtacgttcga acccgggcac
7561 gtgcggacc
//
*Caution
Product is for research use only!
Search name
pEE12.4,Plasmid pEE12.4,pEE12.4 vector