pEE12.4 Plasmid

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  • Model: PVT1803
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pEE12.4

PVT1803  2ug

 

pEE12.4 Information

Promoter: hCMV-MIE
Replicator: Pee6 ori
Terminator: SV40, poly (A) signal
Plasmid classification: mammalian cells, antibody expression vectors
Plasmid size: 7569bp
Prokaryotic resistance: Amp
Clone strain: DH5 alpha
Culture conditions: 37 DEG C, aerobic LB
Expression host: mammalian cells
Induction mode: no induction, transient expression
Primers for 5'sequencing: CMV-F:CGCAAATGGGCGGTAGGCGTG
Primers for 3'sequencing: according to sequences

 

pEE12.4 Description

 

 

pEE12.4 Sequence

LOCUS       Exported                7569 bp ds-DNA     circular SYN 11-JUL-2018

DEFINITION  synthetic circular DNA

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 7569)

  TITLE     Direct Submission

FEATURES             Location/Qualifiers

     source          1..7569

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     polyA_signal    116..237

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(999..1587)

                     /direction=LEFT

                     /label=pEE6 ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(1758..2618)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(2619..2723)

                     /gene="bla"

                     /label=AmpR promoter

     terminator      2892..2919

                     /label=T7Te terminator

                     /note="phage T7 early transcription terminator"

     promoter        3002..3331

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      3182..3317

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     CDS             3370..4491

                     /codon_start=1

                     /label=GS

                     /translation="MATSASSHLNKNIKQMYLCLPQGEKVQAMYIWVDGTGEGLRCKTR

                     TLDCEPKCVEELPEWNFDGSSTFQSEGSNSDMYLSPVAMFRDPFRRDPNKLVFCEVFKY

                     NRKPAETNLRHSCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYYC

                     GVGADKAYGRDIVEAHYRACLYAGVKITGTNAEVMPAQWEFQIGPCEGIRMGDHLWVAR

                     FILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKHIEEAIEKLSKRH

                     RYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASIRIPRTVGQEKKGYFEDR

                     RPSANCDPFAVTEAIVRTCLLNETGDEPFQYKN"

     intron          4623..4688

                     /label=small t intron

                     /note="SV40 (simian virus 40) small t antigen intron"

     CDS             4818..4838

                     /codon_start=1

                     /product="nuclear localization signal of SV40 (simian virus

                     40) large T antigen"

                     /label=SV40 NLS

                     /translation="PKKKRKV"

     polyA_signal    5263..5397

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     enhancer        5966..6345

                     /label=CMV enhancer

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        6346..6549

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     misc_feature    join(7529..7569,1..6)

                     /label=MCS

BASE COUNT         1904 a         1869 c         1805 g         1990 t

ORIGIN

        1 gaattcattg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa

       61 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt

      121 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa

      181 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca

      241 tgtctggcgg ccgcgacctg caggcgcaga actggtaggt atggaagatc cctcgagatc

      301 cattgtgctg gcggtaggcg agcagcgcct gcctgaagct gcgggcattc ccgatcagaa

      361 atgagcgcca gtcgtcgtcg gctctcggca ccgaatgcgt atgattctcc gccagcatgg

      421 cttcggccag tgcgtcgagc agcgcccgct tgttcctgaa gtgccagtaa agcgccggct

      481 gctgaacccc caaccgttcc gccagtttgc gtgtcgtcag accgtctacg ccgacctcgt

      541 tcaacaggtc cagggcggca cggatcactg tattcggctg caactttgtc atgcttgaca

      601 ctttatcact gataaacata atatgtccac caacttatca gtgataaaga atccgcgcca

      661 gcacaatgga tctcgaggtc gagggatctc tagaggatcc tctacgccgg acgcatcgtg

      721 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat

      781 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg

      841 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg

      901 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat

      961 aagggagagc gtcgacctcg ggccgcgttg ctggcgtttt tccataggct ccgcccccct

     1021 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa

     1081 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg

     1141 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca

     1201 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa

     1261 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg

     1321 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg

     1381 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga

     1441 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc

     1501 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag

     1561 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac

     1621 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc

     1681 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag

     1741 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt

     1801 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag

     1861 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca

     1921 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact

     1981 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca

     2041 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg

     2101 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc

     2161 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg

     2221 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca

     2281 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt

     2341 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc

     2401 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc

     2461 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca

     2521 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa

     2581 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat

     2641 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa

     2701 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa

     2761 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctgatggctc

     2821 tttgcggcac ccatcgttcg taatgttccg tggcaccgag gacaaccctc aagagaaaat

     2881 gtaatcacac tggctcacct tcgggtgggc ctttctgcgt ttataaggag acactttatg

     2941 tttaagaagg ttggtaaatt ccttgcggct ttggcagcca agctagatcc ggctgtggaa

     3001 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag

     3061 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag

     3121 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc

     3181 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt

     3241 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg

     3301 aggctttttt ggaggcctag gcttttgcaa aaagctagct tggggccacc gctcagagca

     3361 ccttccacca tggccacctc agcaagttcc cacttgaaca aaaacatcaa gcaaatgtac

     3421 ttgtgcctgc cccagggtga gaaagtccaa gccatgtata tctgggttga tggtactgga

     3481 gaaggactgc gctgcaaaac ccgcaccctg gactgtgagc ccaagtgtgt agaagagtta

     3541 cctgagtgga attttgatgg ctctagtacc tttcagtctg agggctccaa cagtgacatg

     3601 tatctcagcc ctgttgccat gtttcgggac cccttccgca gagatcccaa caagctggtg

     3661 ttctgtgaag ttttcaagta caaccggaag cctgcagaga ccaatttaag gcactcgtgt

     3721 aaacggataa tggacatggt gagcaaccag cacccctggt ttggaatgga acaggagtat

     3781 actctgatgg gaacagatgg gcaccctttt ggttggcctt ccaatggctt tcctgggccc

     3841 caaggtccgt attactgtgg tgtgggcgca gacaaagcct atggcaggga tatcgtggag

     3901 gctcactacc gcgcctgctt gtatgctggg gtcaagatta caggaacaaa tgctgaggtc

     3961 atgcctgccc agtgggagtt ccaaatagga ccctgtgaag gaatccgcat gggagatcat

     4021 ctctgggtgg cccgtttcat cttgcatcga gtatgtgaag actttggggt aatagcaacc

     4081 tttgacccca agcccattcc tgggaactgg aatggtgcag gctgccatac caactttagc

     4141 accaaggcca tgcgggagga gaatggtctg aagcacatcg aggaggccat cgagaaacta

     4201 agcaagcggc accggtacca cattcgagcc tacgatccca aggggggcct ggacaatgcc

     4261 cgtcgtctga ctgggttcca cgaaacgtcc aacatcaacg acttttctgc tggtgtcgcc

     4321 aatcgcagtg ccagcatccg cattccccgg actgtcggcc aggagaagaa aggttacttt

     4381 gaagaccgcc gcccctctgc caattgtgac ccctttgcag tgacagaagc catcgtccgc

     4441 acatgccttc tcaatgagac tggcgacgag cccttccaat acaaaaacta attagacttt

     4501 gagtgatctt gagcctttcc tagttcatcc caccccgccc cagagagatc tttgtgaagg

     4561 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta

     4621 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg

     4681 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg

     4741 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact

     4801 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt

     4861 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg

     4921 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt

     4981 ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt tttcttactc

     5041 cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct

     5101 ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc

     5161 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc

     5221 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct

     5281 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca

     5341 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctag

     5401 cttcgtgtca aggacggtga ctgcagtgaa taataaaatg tgtgtttgtc cgaaatacgc

     5461 gttttgagat ttctgtcgcc gactaaattc atgtcgcgcg atagtggtgt ttatcgccga

     5521 tagagatggc gatattggaa aaatcgatat ttgaaaatat ggcatattga aaatgtcgcc

     5581 gatgtgagtt tctgtgtaac tgatatcgcc atttttccaa aagtgatttt tgggcatacg

     5641 cgatatctgg cgatagcgct tatatcgttt acgggggatg gcgatagacg actttggtga

     5701 cttgggcgat tctgtgtgtc gcaaatatcg cagtttcgat ataggtgaca gacgatatga

     5761 ggctatatcg ccgatagagg cgacatcaag ctggcacatg gccaatgcat atcgatctat

     5821 acattgaatc aatattggcc attagccata ttattcattg gttatatagc ataaatcaat

     5881 attggctatt ggccattgca tacgttgtat ccatatcata atatgtacat ttatattggc

     5941 tcatgtccaa cattaccgcc atgttgacat tgattattga ctagttatta atagtaatca

     6001 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta

     6061 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat

     6121 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg

     6181 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac

     6241 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt

     6301 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg

     6361 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc

     6421 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt

     6481 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata

     6541 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac

     6601 ctccatagaa gacaccggga ccgatccagc ctccgcggcc gggaacggtg cattggaacg

     6661 cggattcccc gtgccaagag tgacgtaagt accgcctata gagtctatag gcccaccccc

     6721 ttggcttctt atgcatgcta tactgttttt ggcttggggt ctatacaccc ccgcttcctc

     6781 atgttatagg tgatggtata gcttagccta taggtgtggg ttattgacca ttattgacca

     6841 ctcccctatt ggtgacgata ctttccatta ctaatccata acatggctct ttgccacaac

     6901 tctctttatt ggctatatgc caatacactg tccttcagag actgacacgg actctgtatt

     6961 tttacaggat ggggtctcat ttattattta caaattcaca tatacaacac caccgtcccc

     7021 agtgcccgca gtttttatta aacataacgt gggatctcca cgcgaatctc gggtacgtgt

     7081 tccggacatg ggctcttctc cggtagcggc ggagcttcta catccgagcc ctgctcccat

     7141 gcctccagcg actcatggtc gctcggcagc tccttgctcc taacagtgga ggccagactt

     7201 aggcacagca cgatgcccac caccaccagt gtgccgcaca aggccgtggc ggtagggtat

     7261 gtgtctgaaa atgagctcgg ggagcgggct tgcaccgctg acgcatttgg aagacttaag

     7321 gcagcggcag aagaagatgc aggcagctga gttgttgtgt tctgataaga gtcagaggta

     7381 actcccgttg cggtgctgtt aacggtggag ggcagtgtag tctgagcagt actcgttgct

     7441 gccgcgcgcg ccaccagaca taatagctga cagactaaca gactgttcct ttccatgggt

     7501 cttttctgca gtcaccgtcc ttgacacgaa gcttcgcgac gtacgttcga acccgggcac

     7561 gtgcggacc

//

 

*Caution 

Product is for research use only!
 

Search name

pEE12.4,Plasmid pEE12.4,pEE12.4 vector

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