pEnCMV-MCS-3×FLAG
PVT23125 2ug
pEnCMV-MCS-3×FLAG Informaiton
Alias: pCDNA3.4-3×FLAG-C
Prokaryotic resistance:Amp
Screening marker: G418
Clone strain: DH5 α
Culture condition: LB / 37 ℃
pEnCMV-MCS-3×FLAG Description
pEnCMV-MCS-3×FLAG is a mammalian expression plasmid, which size is 6046bp, resistance is Amp.
pEnCMV-MCS-3×FLAG Sequence
LOCUS Exported 6046 bp ds-DNA circular SYN 23-JUL-2019
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..6046
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 50..429
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 430..633
/label=CMV
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 637..732
/label=MCS
CDS 733..798
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
misc_feature 822..1410
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
polyA_signal 1476..1524
/label=HSV TK poly(A)
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 1726..2154
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2168..2497
/label=SV40
/note="SV40 enhancer and early promoter"
rep_origin 2348..2483
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2564..3358
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=G418
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3534..3655
/label=SV40 poly(A)
/note="SV40 polyadenylation signal"
rep_origin complement(4106..4694)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4865..5725)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5726..5830)
/gene="bla"
/label=AmpR promoter
BASE COUNT 1351 a 1615 c 1543 g 1536 t
ORIGIN
1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
661 taccgagctc ggatccacta gtccagtgtg gtggaattct gcagatatcc agcacagtgg
721 cggccgctcg aggactacaa agaccatgac ggtgattata aagatcatga catcgactac
781 aaggatgacg atgacaagta gtgagggccc gatatctcga caatcaacct ctggattaca
841 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
901 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
961 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
1021 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
1081 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
1141 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
1201 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
1261 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
1321 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
1381 gtcggatctc cctttgggcc gcctccccgc ctggaaacgg gggaggctaa ctgaaacacg
1441 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
1501 gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
1561 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
1621 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
1681 ctgccatagc agatctgcgc agctggggct ctagggggta tccccacgcg ccctgtagcg
1741 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
1801 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
1861 cccgtcaagc tctaaatcgg ggcatccctt tagggttccg atttagtgct ttacggcacc
1921 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
1981 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
2041 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgggga
2101 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
2161 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
2221 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
2281 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
2341 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
2401 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
2461 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
2521 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
2581 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
2641 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
2701 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
2761 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
2821 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
2881 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
2941 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
3001 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
3061 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
3121 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
3181 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
3241 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
3301 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
3361 gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
3421 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
3481 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
3541 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
3601 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
3661 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
3721 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
3781 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
3841 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
3901 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
3961 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
4021 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
4081 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
4141 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
4201 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
4261 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
4321 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
4381 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
4441 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
4501 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
4561 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
4621 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
4681 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
4741 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
4801 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
4861 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
4921 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
4981 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
5041 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
5101 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
5161 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
5221 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
5281 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
5341 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
5401 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
5461 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
5521 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
5581 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
5641 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
5701 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
5761 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
5821 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
5881 cgatccccta tggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta
5941 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
6001 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagg
//
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