pENTR223-STXBP1
Catalog No. PVT10000
Packing 2ug
Function DNA Cloning plasmid
Resistance Spe
Screen /
Strain DH5alpha
Culture temperature 37degrees centigrade
Replicon pBR322
Copy Middle
Promoter -
Induction -
Forward primer M13F
Reverse primer M13R
pENTR223-STXBP1 Sequence
LOCUS Exported 4574 bp ds-DNA circular SYN 05-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..4574
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 268..295
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 387..473
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
protein_bind 570..668
/gene="mutant version of attL"
/label=attL1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
misc_feature 669..2451
/label=STXBP1
/note="NM_001032221.3"
protein_bind complement(2452..2551)
/gene="mutant version of attL"
/label=attL2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
CDS 3040..3831
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=Spe
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 3924..4512
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
541 aacgacggcc agtcttaagc tcgggccccc aaataatgat tttattttga ctgatagtga
601 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
661 aagttggcat ggcccccatt ggcctcaaag ctgttgtcgg agagaagatt atgcatgatg
721 tgataaagaa ggtcaagaag aagggggaat ggaaggtgct ggtggtggat cagttaagca
781 tgaggatgct gtcctcctgc tgcaagatga cagacatcat gaccgagggc ataacgattg
841 tggaagatat caataagcgc agagagccgc tccccagcct ggaggctgtg tatctcatca
901 ctccatccga gaagtccgtc cactctctca tcagtgactt taaggacccg ccgactgcta
961 aataccgggc tgcacacgtc ttcttcactg actcttgtcc agatgccctg tttaatgaac
1021 tggtaaaatc ccgagcagcc aaagtcatca aaactctgac ggaaatcaat attgcatttc
1081 tcccgtatga atcccaggtc tattccttgg actctgctga ctctttccaa agcttctaca
1141 gtccccacaa ggctcagatg aagaatccta tactggagcg cctggcagag cagatcgcga
1201 ccctttgtgc caccctgaag gagtacccgg ctgtgcggta tcggggggaa tacaaggaca
1261 atgccctgct ggctcagcta atccaggaca agctcgatgc ctataaagct gatgatccaa
1321 caatggggga gggcccagac aaggcacgct cccagctcct gatcctggat cgaggctttg
1381 accccagctc ccctgtgctc catgaattga cttttcaggc tatgagttat gatctgctgc
1441 ctatcgaaaa tgatgtatac aagtatgaga ccagcggcat cggggaggca cgggtgaagg
1501 aggtgctcct ggacgaggac gacgacctgt ggatagcact gcgccacaag cacatcgcag
1561 aggtgtccca ggaagtcacc cggtctctga aagatttttc ttctagcaag agaatgaata
1621 ctggagagaa gaccaccatg cgggacctgt cccagatgct gaagaagatg cctcagtacc
1681 agaaagagct cagcaagtac tccacccacc tgcaccttgc tgaggactgt atgaagcatt
1741 accaaggcac cgtagacaaa ctctgccgag tggagcagga cctggccatg ggcacagatg
1801 ctgagggaga gaagatcaag gaccctatgc gagccatcgt ccccattctg ctggatgcca
1861 atgtcagcac ttatgacaaa atccgcatca tccttctcta catctttttg aagaatggca
1921 tcacggagga aaacctgaac aaactgatcc agcacgccca gatacccccg gaggatagtg
1981 agatcatcac caacatggct cacctcggcg tgcccatcgt caccgattcc acgctgcgtc
2041 gccggagcaa gccggagcgg aaggaacgca tcagcgagca gacctaccag ctctcacggt
2101 ggactccgat tatcaaggac atcatggagg acactattga ggacaaactt gacaccaaac
2161 actaccctta tatctctacc cgttcctctg cctccttcag caccaccgcc gtcagcgccc
2221 gctatgggca ctggcataag aacaaggccc caggcgagta ccgcagtggc ccccgcctca
2281 tcattttcat ccttgggggt gtgagcctga atgagatgcg ctgcgcctac gaggtgaccc
2341 aggccaacgg aaagtgggag gtgctgatag gatccacaca catcctcacc ccacagaaac
2401 tgctggacac actgaagaaa ctgaataaaa cagatgaaga aataagcagt tacccaactt
2461 tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg
2521 tcactatcag tcaaaataaa atcattattt ggccatccag ctgatatccc ctatagtgag
2581 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
2641 tgttacattg cacaagataa aaatatatca tcatgcctcc tctagaccag ccaggacaga
2701 aatgcctcga cttcgctgct gcccaaggtt gccgggtgac gcacaccgtg gaaacggatg
2761 aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt
2821 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
2881 gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc
2941 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
3001 acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc
3061 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
3121 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat
3181 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
3241 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
3301 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
3361 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc
3421 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
3481 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
3541 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
3601 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
3661 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat actt
