pENTR223-TIGIT
Catalog No. PVT10003
Packing 2ug
pENTR223-TIGIT Information
Function DNA Cloning plasmid
Resistance Spe
Screen /
Strain DH5alpha
Culture temperature 37degrees centigrade
Replicon pBR322
Copy Middle
Promoter -
Induction -
Forward primer M13F
Reverse primer M13R
Alternative name: NM_173799.3，VSIG9; VSTM3; WUCAM
pENTR223-TIGIT Sequence
LOCUS Exported 3525 bp ds-DNA circular SYN 05-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..3525
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 268..295
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 387..473
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
protein_bind 569..668
/gene="mutant version of attL"
/label=attL1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
misc_feature 671..1402
/label=TIGIT
/note="NM_173799.3"
protein_bind complement(1404..1503)
/gene="mutant version of attL"
/label=attL2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
CDS 1991..2782
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=Spe
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 2875..3463
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
661 agttggcacc atgcgctggt gtctcctcct gatctgggcc caggggctga ggcaggctcc
721 cctcgcctca ggaatgatga caggcacaat agaaacaacg gggaacattt ctgcagagaa
781 aggtggctct atcatcttac aatgtcacct ctcctccacc acggcacaag tgacccaggt
841 caactgggag cagcaggacc agcttctggc catttgtaat gctgacttgg ggtggcacat
901 ctccccatcc ttcaaggatc gagtggcccc aggtcccggc ctgggcctca ccctccagtc
961 gctgaccgtg aacgatacag gggagtactt ctgcatctat cacacctacc ctgatgggac
1021 gtacactggg agaatcttcc tggaggtcct agaaagctca gtggctgagc acggtgccag
1081 gttccagatt ccattgcttg gagccatggc cgcgacgctg gtggtcatct gcacagcagt
1141 catcgtggtg gtcgcgttga ctagaaagaa gaaagccctc agaatccatt ctgtggaagg
1201 tgacctcagg agaaaatcag ctggacagga ggaatggagc cccagtgctc cctcaccccc
1261 aggaagctgt gtccaggcag aagctgcacc tgctgggctc tgtggagagc agcggggaga
1321 ggactgtgcc gagctgcatg actacttcaa tgtcctgagt tacagaagcc tgggtaactg
1381 cagcttcttc acagagactg gtttgccaac tttcttgtac aaagttggca ttataagaaa
1441 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
1501 ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg
1561 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
1621 atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc tgcccaaggt
1681 tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg
1741 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
1801 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
1861 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
1921 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
1981 gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
2041 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
2101 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
2161 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
2221 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
2281 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
2341 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
2401 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
2461 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
2521 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
2581 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
2641 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
2701 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
2761 caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc ttaacgtgag
2821 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
2881 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
2941 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
3001 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
3061 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
3121 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
3181 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
3241 cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa
3301 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
3361 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
3421 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
3481 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//
Caution:
Product is for research use only!
