pET-23b

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pET-23b,Plasmid pET-23b,pET-23b vector

pET-23b information

Bacterial Resistance:Ampicillin
Growth Strain:DH5α
Expression: Bacterial
Use:pET Plasmid

​pET-23b Description

pET-23a-d(+) vectors carry an N-terminal T7•Tag sequence plus an optional C-terminal His•Tag® sequence. These vectors differ from pET-21a-d(+) by the “plain” T7 promoter instead of the T7lac promoter and by the absence of the lacI gene. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer.

Reference
Discovery of an acidic, thermostable and highly NADP+ dependent formate dehydrogenase from Lactobacillus buchneri NRRL B-30929
Author
Saadet AlpdağtaşSevil YücelHandan Açelya KapkaçSiqing LiuBarış Binay 
Email [email protected]
DOI http://dx.doi.org/10.1007/s10529-018-2568-6
Publisher Name Springer Netherlands
Print ISSN 0141-5492
Online ISSN 1573-6776