Plasmid type:E.coli Expression VectorCopy number:High copyCloning Method:Multiple cloning sites,restriction endonucleaseSize:5422 bp 5' Sequencing primers and sequences:T7: 5'-TAATACGACTCACTATAGGG-3'3' Sequencing primer sequence:??T7t: 5'-GCTAGTTATTGCTCAGCGG-3'Tags:N-His, N-S, N-Thrombin, N-EK, C-HisResistance(s):Kanamycin (Kan)Note:The difference of pet30a, pet30b and pet30c is mainly in multiple clone sites. |