pET32a-MS2(V00642.1)
Catalog No. PVT10542
Packing 2ug
pET32a-MS2 Information
Function Other source plasmids
Resistance Amp
Screen /
Strain Stbl3
Culture temperature 37degrees centigrade
pET32a-MS2 Description
PET32a-MS2 is a cloning plasmid of E. coli bacteriophage gene.
pET32a-MS2 Sequence
LOCUS Exported 7678 bp ds-DNA circular SYN 25-SEP-2018
DEFINITION synthetic circular DNA
KEYWORDS pET32a-MS2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7678)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..7678
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 12..467
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 494..598
/gene="bla"
/note="AmpR promoter"
CDS 599..1459
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1630..2218
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 2404..2546
/note="bom"
/note="basis of mobility region from pBR322"
CDS complement(2648..2839)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/note="rop"
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
CDS complement(3648..4730)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/note="lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
promoter complement(4731..4808)
/gene="lacI"
/note="lacI promoter"
promoter 5121..5139
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 5140..5164
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 5179..5201
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 5209..5535
/codon_start=1
/gene="trxA"
/product="E. coli thioredoxin"
/note="TrxA"
/translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
DANLA"
CDS 5557..5574
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
CDS 5608..5652
/codon_start=1
/product="affinity and epitope tag derived from pancreatic
ribonuclease A"
/note="S-Tag"
/translation="KETAAAKFERQHMDS"
misc_feature 5704..7500
/note="MS2"
/note="phage MS2 genomeGenBank: V00642.1"
terminator 7606..7653
/note="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
ORIGIN
1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
1141 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg
2461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
2521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
2581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
2641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
2701 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
2761 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
2821 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa
2881 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc
2941 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa
3001 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta
3061 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg
3121 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag
3181 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac
3241 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca
3301 cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg
3361 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc
3421 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg
3481 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca
3541 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag
3601 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt
3661 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
3721 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc
3781 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc
3841 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct
3901 tcggtatcgt cgtatcccac taccgagatg tccgcaccaa cgcgcagccc ggactcggta
3961 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg
4021 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct
4081 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga
4141 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc
4201 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg
4261 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct
4321 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt
4381 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc
4441 gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc
4501 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc
4561 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact
4621 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga
4681 taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc
4741 ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg
4801 atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag
4861 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc
4921 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat
4981 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc
5041 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat
5101 cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa
5161 ttcccctcta gaaataattt tgtttaactt taagaaggag atatacatat gagcgataaa
5221 attattcacc tgactgacga cagttttgac acggatgtac tcaaagcgga cggggcgatc
5281 ctcgtcgatt tctgggcaga gtggtgcggt ccgtgcaaaa tgatcgcccc gattctggat
5341 gaaatcgctg acgaatatca gggcaaactg accgttgcaa aactgaacat cgatcaaaac
5401 cctggcactg cgccgaaata tggcatccgt ggtatcccga ctctgctgct gttcaaaaac
5461 ggtgaagtgg cggcaaccaa agtgggtgca ctgtctaaag gtcagttgaa agagttcctc
5521 gacgctaacc tggccggttc tggttctggc catatgcacc atcatcatca tcattcttct
5581 ggtctggtgc cacgcggttc tggtatgaaa gaaaccgctg ctgctaaatt cgaacgccag
5641 cacatggaca gcccagatct gggtaccgac gacgacgaca aggccatggc tgatatcgga
5701 tcccctttcg gggtcctgct caacttcctg tcgagctaat gccattttta atgtctttag
5761 cgagacgcta ccatggctat cgctgtaggt agccggaatt ccattcctag gaggtttgac
5821 ctgtgcgagc ttttagtacc cttgataggg agaacgagac cttcgtcccc tccgttcgcg
5881 tttacgcgga cggtgagact gaagataact cattctcttt aaaatatcgt tcgaactgga
5941 ctcccggtcg ttttaactcg actggggcca aaacgaaaca gtggcactac ccctctccgt
6001 attcacgggg ggcgttaagt gtcacatcga tagatcaagg tgcctacaag cgaagtgggt
6061 catcgtgggg tcgcccgtac gaggagaaag ccggtttcgg cttctccctc gacgcacgct
6121 cctgctacag cctcttccct gtaagccaaa acttgactta catcgaagtg ccgcagaacg
6181 ttgcgaaccg ggcgtcgacc gaagtcctgc aaaaggtcac ccagggtaat tttaaccttg
6241 gtgttgcttt agcagaggcc aggtcgacag cctcacaact cgcgacgcaa accattgcgc
6301 tcgtgaaggc gtacactgcc gctcgtcgcg gtaattggcg ccaggcgctc cgctaccttg
6361 ccctaaacga agatcgaaag tttcgatcaa aacacgtggc cggcaggtgg ttggagttgc
6421 agttcggttg gttaccacta atgagtgata tccagggtgc atatgagatg cttacgaagg
6481 ttcaccttca agagtttctt cctatgagag ccgtacgtca ggtcggtact aacatcaagt
6541 tagatggccg tctgtcgtat ccagctgcaa acttccagac aacgtgcaac atatcgcgac
6601 gtatcgtgat atggttttac ataaacgatg cacgtttggc atggttgtcg tctctaggta
6661 tcttgaaccc actaggtata gtgtgggaaa aggtgccttt ctcattcgtt gtcgactggc
6721 tcctacctgt aggtaacatg ctcgagggcc ttacggcccc cgtgggatgc tcctacatgt
6781 caggaacagt tactgacgta ataacgggtg agtccatcat aagcgttgac gctccctacg
6841 ggtggactgt ggagagacag ggcactgcta aggcccaaat ctcagccatg catcgagggg
6901 tacaatccgt atggccaaca actggcgcgt acgtaaagtc tcctttctcg atggtccata
6961 ccttagatgc gttagcatta atcaggcaac ggctctccag atagagccct caaccggagt
7021 ttgaagcatg gcttctaact ttactcagtt cgttctcgtc gacaatggcg gaactcacca
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