pEVOL-pBpF
Catalog No. PVT10539
Packing 2ug
pEVOL-pBpF Information
Function Other source plasmids
Resistance /
Screen /
Strain /
Culture temperature
Replicon
Copy
Promoter
Induction
Forward primer
Reverse primer
pEVOL-pBpF Sequence
LOCUS Exported 6128 bp ds-DNA circular SYN 27-SEP-2017
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6128)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 27, 2017
FEATURES Location/Qualifiers
source 1..6128
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 22..306
/gene="araBAD"
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
CDS 343..1263
/codon_start=1
/label=M.j. p-benzoylphenylalanine RS (2 copies +tRNA)
/translation="MDEFEMIKRNTSEIISEEELREVLKKDEKSAGIGFEPSGKIHLGH
YLQIKKMIDLQNAGFDIIILLADLHAYLNQKGELDEIRKIGDYNKKVFEAMGLKAKYLY
GSPFQLDKDYTLNVYRLALKTTLKRARRSMELIAREDENPKVAEVIYPIMQVNTSHYLG
VDVAVGGMEQRKIHMLARELLPKKVVCIHNPVLTGLDGEGKMSSSKGNFIAVDDSPEEI
RAKIKKAYCPAGVVEGNPIMEIAKYFLEYPLTIKRPEKFGGDLTVNSYEELESLFKNKE
LHPMDLKNAVAEELIKILEPIRKRL"
CDS complement(3266..3925)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
promoter complement(3926..4028)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
rep_origin complement(4554..5099)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
CDS complement(5245..6123)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/label=araC
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPSDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
ORIGIN
1 tcatactccc gccattcaga gaagaaacca attgtccata ttgcatcaga cattgccgtc
61 actgcgtctt ttactggctc ttctcgctaa ccaaaccggt aaccccgctt attaaaagca
121 ttctgtaaca aagcgggacc aaagccatga caaaaacgcg taacaaaagt gtctataatc
181 acggcagaaa agtccacatt gattatttgc acggcgtcac actttgctat gccatagcat
241 ttttatccat aagattagcg gatcctacct gacgcttttt atcgcaactc tctactgttt
301 ctccataccc gtttttttgg gctaacagga ggaattagat ctatggacga atttgaaatg
361 ataaagagaa acacatctga aattatcagc gaggaagagt taagagaggt tttaaaaaaa
421 gatgaaaaat ctgctggtat aggttttgaa ccaagtggta aaatacattt agggcattat
481 ctccaaataa aaaagatgat tgatttacaa aatgctggat ttgatataat tatattgttg
541 gctgatttac acgcctattt aaaccagaaa ggagagttgg atgagattag aaaaatagga
601 gattataaca aaaaagtttt tgaagcaatg gggttaaagg caaaatatct ttatggaagt
661 cctttccagc ttgataagga ttatacactg aatgtctata gattggcttt aaaaactacc
721 ttaaaaagag caagaaggag tatggaactt atagcaagag aggatgaaaa tccaaaggtt
781 gctgaagtta tctatccaat aatgcaggtt aatacgagtc attatctggg cgttgatgtt
841 gcagttggag ggatggagca gagaaaaata cacatgttag caagggagct tttaccaaaa
901 aaggttgttt gtattcacaa ccctgtctta acgggtttgg atggagaagg aaagatgagt
961 tcttcaaaag ggaattttat agctgttgat gactctccag aagagattag ggctaagata
1021 aagaaagcat actgcccagc tggagttgtt gaaggaaatc caataatgga gatagctaaa
1081 tacttccttg aatatccttt aaccataaaa aggccagaaa aatttggtgg agatttgaca
1141 gttaatagct atgaggagtt agagagttta tttaaaaata aggaattgca tccaatggat
1201 ttaaaaaatg ctgtagctga agaacttata aagattttag agccaattag aaagagatta
1261 taataagtcg accatcatca tcatcatcat tgagtttaaa cggtctccag cttggctgtt
1321 ttggcggatg agagaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc
1381 tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga
1441 actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag
1501 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc cttgtttgtg
1561 agctcccggt catcaatcat ccccataatc cttgttagat tatcaatttt aaaaaactaa
1621 cagttgtcag cctgtcccgc tttaatatca tacgccgtta tacgttgttt acgctttgag
1681 gaatcccata tggacgaatt tgaaatgata aagagaaaca catctgaaat tatcagcgag
1741 gaagagttaa gagaggtttt aaaaaaagat gaaaaatctg ctggtatagg ttttgaacca
1801 agtggtaaaa tacatttagg gcattatctc caaataaaaa agatgattga tttacaaaat
1861 gctggatttg atataattat attgttggct gatttacacg cctatttaaa ccagaaagga
1921 gagttggatg agattagaaa aataggagat tataacaaaa aagtttttga agcaatgggg
1981 ttaaaggcaa aatatcttta tggaagtcct ttccagcttg ataaggatta tacactgaat
2041 gtctatagat tggctttaaa aactacctta aaaagagcaa gaaggagtat ggaacttata
2101 gcaagagagg atgaaaatcc aaaggttgct gaagttatct atccaataat gcaggttaat
2161 acgagtcatt atctgggcgt tgatgttgca gttggaggga tggagcagag aaaaatacac
2221 atgttagcaa gggagctttt accaaaaaag gttgtttgta ttcacaaccc tgtcttaacg
2281 ggtttggatg gagaaggaaa gatgagttct tcaaaaggga attttatagc tgttgatgac
2341 tctccagaag agattagggc taagataaag aaagcatact gcccagctgg agttgttgaa
2401 ggaaatccaa taatggagat agctaaatac ttccttgaat atcctttaac cataaaaagg
2461 ccagaaaaat ttggtggaga tttgacagtt aatagctatg aggagttaga gagtttattt
2521 aaaaataagg aattgcatcc aatggattta aaaaatgctg tagctgaaga acttataaag
2581 attttagagc caattagaaa gagattataa ctgcagtttc aaacgctaaa ttgcctgatg
2641 cgctacgctt atcaggccta catgatctct gcaatatatt gagtttgcgt gcttttgtag
2701 gccggataag gcgttcacgc cgcatccggc aagaaacagc aaacaatcca aaacgccgcg
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