pFGC5941
Catalog No. PVT11220
Packing 2ug
pFGC5941 Multiple cloning site
pFGC5941 Information
Promoter: CaMV 35S
Replicon: pVS1 oriV, ori
Plasmid classification: plant series, protein overexpression vector
Plasmid size: 11406bp
Prokaryotic resistance: Kan
Clonal strain: DH5 alpha
Culture conditions: 37 C, aerobic LB
Expression host: plant cells
5'sequencing primers: 35S:GACGCACAATCCCACTATCC
Primers for 3'sequencing: primers designed based on sequences
pFGC5941 Sequence
LOCUS Exported 11406 bp ds-DNA circular SYN 11-JAN-2016
DEFINITION Agrobacterium binary vector for expressing dsRNA, with two multiple
cloning sites separated by an intron.
ACCESSION AY310901
VERSION .
KEYWORDS pFGC5941
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11406)
AUTHORS Kerschen A, Napoli CA, Jorgensen RA, Muller AE.
TITLE Effectiveness of RNA interference in transgenic plants.
JOURNAL FEBS Lett. 2004;566:223-8.
PUBMED 15147899
REFERENCE 2 (bases 1 to 11406)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.2.1
COMMENT The GenBank sequence was corrected by inserting a G at position
7514.
FEATURES Location/Qualifiers
source 1..11406
/organism="synthetic DNA construct"
/lab_host="Plant Cells"
/mol_type="other DNA"
misc_feature 1..25
/note="LB T-DNA repeat"
/note="left border repeat from nopaline C58 T-DNA"
terminator 111..363
/note="MAS terminator"
/note="mannopine synthase terminator"
CDS complement(373..924)
/codon_start=1
/gene="Streptomyces hygroscopicus bar"
/product="phosphinothricin acetyltransferase"
/note="BlpR"
/note="confers resistance to bialophos or phosphinothricin"
/translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
WQLDFSLPVPPRPVLPVTEI"
promoter complement(930..1310)
/note="MAS promoter"
/note="mannopine synthase promoter (Velten et al., 1984)"
promoter 2305..2650
/note="CaMV 35S promoter"
/note="strong constitutive promoter from cauliflower mosaic
virus"
misc_feature 2680..2734
/note="TMV Omega"
/note="translational enhancer from the tobacco mosaic virus
5'-leader sequence (Gallie et al., 1988)"
misc_feature 2734..2764
/note="MCS 1"
/note="multiple cloning site 1"
intron 2767..4115
/gene="chsA"
/note="chsA intron"
/note="chalcone synthase A intron from Petunia hybrida"
misc_feature 4122..4160
/note="MCS 2"
/note="multiple cloning site"
terminator 4177..4884
/note="OCS terminator"
/note="octopine synthase terminator"
misc_feature 5128..5152
/note="RB T-DNA repeat"
/note="right border repeat from nopaline C58 T-DNA"
CDS 6452..7081
/codon_start=1
/product="stability protein from Pseudomonas plasmid pVS1"
/note="pVS1 StaA"
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 7510..8583
/codon_start=1
/product="replication protein from Pseudomonas plasmid
pVS1"
/note="pVS1 RepA"
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 8649..8843
/note="pVS1 oriV"
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 9187..9327
/note="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(9513..10101)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(10188..10982)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/note="KanR"
&nb