pGBKT7- BD Plasmid


  • Model: PVT4017
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pGBKT7-BD,Plasmid pGBKT7-BD,pGBKT7-BD vector


pGBKT7-BD Information

Bacterial Resistance:Kanamycin
Growth Strain:
Expression: Yeast
Use: Yeast expression


pGBKT7-BD Description

pGBKT7/BD (pEXT03/BD) vector expresses proteins fused to amino acids 1–147 of the GAL4 DNA binding domain (DNA-BD) for constructing fusion proteins with a bait protein. pGBKT7 replicates autonomously in both E. coli and S.cerevisiae from the pUC and 2 μ ori, respectivel



pGBKT7-BD Sequence

LOCUS       Exported                7304 bp ds-DNA     circular SYN 10-SEP-2016
DEFINITION  synthetic circular DNA
KEYWORDS    Untitled 19
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7304)
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, September 10, 2016 from SnapGene Viewer 3.1.4
FEATURES             Location/Qualifiers
     source          1..7304
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        30..734
                     /gene="S. cerevisiae ADH1"
                     /note="ADH1 promoter"
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             762..1202
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     /note="GAL4 DNA binding domain"
     promoter        1213..1231
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1251..1280
                     /product="Myc (human c-Myc oncogene) epitope tag"
     terminator      1338..1385
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
     terminator      1412..1599
                     /gene="S. cerevisiae ADH1"
                     /note="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1623..1639)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
     protein_bind    1647..1663
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1671..1701)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(2025..2613)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
     polyA_signal    2942..2989
                     /note="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(3222..4016)
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="confers resistance to neomycin, kanamycin, and G418 
     promoter        complement(4017..4121)
                     /note="AmpR promoter"
     rep_origin      4148..5490
                     /note="2u ori"
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(5111..5158)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        5749..6030
                     /gene="S. cerevisiae TRP1"
                     /note="TRP1 promoter"
     CDS             6031..6705
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /note="yeast auxotrophic marker"
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