pGL3- U6- sgRNA- PGK- puromycin

$193.00

  • Model: PVT10975
  • 50 Units in Stock
Ask a question


Add to Cart:

pGL3-U6-sgRNA-PGK-puromycin 

PVT10975  2ug

 

pGL3-U6-sgRNA-PGK-puromycin Information

Nuclear resistance: ampicillin Amp

Screening marker: puromycin Puro

Clone strain: Escherichia coli DH5 alpha

Culture conditions: LB medium, 37 C

Remarks: there are differences between sequences of Addgene.

 

pGL3-U6-sgRNA-PGK-puromycin Description

PGL3-U6-sgRNA-PGK-puromycin is a mammalian gene editing plasmid. U6 promotes the transcription of sgRNA, which can be digested by BsaI and linked to sgRNA sequence. pGK promotes the expression of purinomycin Puro resistance gene.

 

pGL3-U6-sgRNA-PGK-puromycin Multiple cloning site

pGL3-U6-sgRNA-PGK-puromycin-vector

 

pGL3-U6-sgRNA-PGK-puromycin Sequence

LOCUS       Exported                4951 bp ds-DNA    circular SYN 17-JULY-2017
KEYWORDS    pGL3-U6-sgRNA-PGK-puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4951)
  AUTHORS    
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        72..312
                     /label="U6 Promoter"
                     /note="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        343..418
                     /note="gRNA scaffold"
                     /note="guide RNA scaffold for the CRISPR/Cas9 system"
     misc_feature    470..587
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        636..1146
                     /note="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             1168..1767
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    1914..2035
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2454..3042)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3213..4073)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4074..4178)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      4205..4660
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     polyA_signal    4791..4839
                     /note="synthetic polyadenylation signal"
     misc_feature    4853..4944
                     /note="pause site"
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgatt agtgaacgga tctcgacggt atcgatcacg agactagcct cgagcggccg
       61 cccccttcac cgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
      121 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
      181 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
      241 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
      301 tgtggaaagg acgaaacacc gtgagaccga gagagggtct cagttttaga gctagaaata
      361 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
      421 tttttaaaga attctcgacc tcgagacaaa tggcagtatt catccacaat tttaaaagaa
      481 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
      541 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
      601 gggacagcag agatccactt tggccgcggc tcgagggggt tggggttgcg ccttttccaa
      661 ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc
      721 ggcgccgacc ctgggactcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc
      781 gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt
      841 tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc
      901 ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc
      961 aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag
     1021 gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc
     1081 aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct
     1141 ccccaggggg atccaccgga gcttaccatg accgagtaca agcccacggt gcgcctcgcc
     1201 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     1261 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     1321 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     1381 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     1441 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     1501 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     1561 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     1621 gagcgcgccg gggtgcccgc cttcctggaa acctccgcgc cccgcaacct ccccttctac
     1681 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     1741 tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa
     1801 aggagcgcac gaccccatgc atcggtacct ttaagaccaa tgacttacaa ggcagctgta
     1861 gatcttagcc actttctaga gtcggggcgg ccggccgctt cgagcagaca tgataagata
     1921 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     1981 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
No customer comments for the moment.

Add A Comment

Related Products

Cart  

No products

Total $0.00

Prices don't include postage.

Cart