pGreen II 62-SK

$246.00

  • Model: PVT3026
  • 46 Units in Stock
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pGreen II 62-SK

PVT3026   2ug                                    Lot no 1901093

Store at -20                                      Expiry date April.2020

 

pGreen II 62-SK  Information

Promoter: CaMV 35S
Carrier size: 3347 BP
Carrier resistance: Kanamycin
Expression: Plant
Use: Plant expression

 

pGreen II 62-SK  Sequences

LOCUS Exported 3347 bp ds-DNA circular SYN 14-SEP-2016

DEFINITION synthetic circular DNA

ACCESSION .

VERSION .

KEYWORDS Untitled 10

SOURCE synthetic DNA construct

ORGANISM synthetic DNA construct

REFERENCE 1 (bases 1 to 3347)

AUTHORS .

TITLE Direct Submission

JOURNAL Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.2.1

FEATURES Location/Qualifiers

source 1..3347

/organism="synthetic DNA construct"

/mol_type="other DNA"

misc_feature 542..564

/note="LB T-DNA repeat"

/note="left border repeat from nopaline C58 T-DNA

(truncated)"

promoter 638..982

/note="CaMV 35S promoter"

/note="strong constitutive promoter from cauliflower mosaic

virus"

misc_feature 992..1099

/note="MCS"

/note="pBluescript multiple cloning site"

primer_bind 1016..1032

/note="SK primer"

/note="common sequencing primer, one of multiple similar

variants"

primer_bind complement(1066..1082)

/note="KS primer"

/note="common sequencing primer, one of multiple similar

variants"

polyA_signal 1153..1329

/note="CaMV poly(A) signal"

/note="cauliflower mosaic virus polyadenylation signal"

misc_feature 1339..1363

/note="RB T-DNA repeat"

/note="right border repeat from nopaline C58 T-DNA"

rep_origin complement(1454..2042)

/direction=LEFT

/note="ori"

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

CDS complement(2213..3028)

/codon_start=1

/gene="aph(3')-Ia"

/product="aminoglycoside phosphotransferase"

/note="KanR"

/note="confers resistance to kanamycin in bacteria or G418

(Geneticin(R)) in eukaryotes"

/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG

KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK

TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA

SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI

ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"

rep_origin 3319..407

/note="pSa ori"

/note="origin of replication from bacterial plasmid pSa"

ORIGIN

1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc

61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg

121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg

181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc

241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat

301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac

361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc

421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa

481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagagatc

541 ttggcaggat atattgtggt gtaacgttat cagcttggta cgtacccccc tactccaaaa

601 atgtcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa

661 tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag

721 tagaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc

781 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg

841 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgac atctccactg

901 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa

961 gtcatttcat ttggagagga cagcccaagc tgagctccac cgcggtggcg gccgctctag

1021 aactagtgga tcccccgggc tgcaggaatt cgatatcaag cttatcgata ccgtcgacct

1081 cgaggggggg cccggtacca attcggtacg ctgaaatcac cagtctctct ctacaaatct

1141 atctctctct attttctcca taaataatgt gtgagtagtt tcccgataag ggaaattagg

1201 gttcttatag ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta

1261 tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc cagtactaaa

1321 atccagatcc actagccttg acaggatata ttggcgggta aactaagtcg ctgtatgtgt

1381 ttgtttgaga tctcatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg

1441 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct

1501 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa

1561 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc

1621 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt

1681 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg

1741 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg

1801 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct

1861 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc

1921 tgaagccagt taccttcgga agaagagttg gtagctcttg atccggcaaa caaaccaccg

1981 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc

2041 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt

2101 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa

2161 aatgaagttt taaatcaatc taaagtatat atgtgtaaca ttggtctagt gattagaaaa

2221 actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt

2281 tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg

2341 caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt

2401 tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg

2461 gtgagaatgg caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac

2521 gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag

2581 cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc

2641 ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta

2701 atacctggaa tgctgttttc cctgggatcg cagtggtgag taaccatgca tcatcaggag

2761 tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga

2821 ccatctcatc tgtaacaaca ttggcaacgc tacctttgcc atgtttcaga aacaactctg

2881 gcgcatcggg cttcccatac aatcggtaga ttgtcgcacc tgattgcccg acattatcgc

2941 gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggccttgagc

3001 aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag

3061 acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt

3121 gagacacaac gtggctttgt tgaataaatc gaacttttgc tgagttgaag gatcagatca

3181 cgcatcttcc cgacaacgca gaccgttccg tggcaaagca aaagttcaaa atcaccaact

3241 ggtccaccta caacaaagct ctcatcaacc gtggctccct cactttctgg ctggatgatg

3301 gggcgattca ggcgatcccc atccaacagc ccgccgtcga gcgggct

//

 

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