pGWB11
Catalog No. PVT11150
Packing 2ug
pGWB11 Description
Function plant expression plasmid
Prokaryotic resistance: Kan
Screening markers: hyg, G418
Clone strain: DB3.1
Culture condition: 37 ℃
Host: plant
Application of vector: protein expression
Gene species:-
Gene type:-
Prokaryotic resistance: Kan
Eukaryotic resistance: G418, hyg
pGWB11 Sequence
LOCUS Exported 17260 bp ds-DNA circular SYN 25-JAN-2018
FEATURES Location/Qualifiers
source 1..17260
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(145..516)
/codon_start=1
/gene="traJ"
/product="oriT-recognizing protein"
/label=traJ
/translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
KQDELGKVMMGVVRPRAEP"
oriT 549..658
/note="incP origin of transfer"
misc_feature 2454..2478
/label=RB
/note="right border repeat from nopaline C58 T-DNA"
promoter 2634..2817
/function="NOS promoter"
/label=NOS
/note="nopaline synthase promoter"
CDS 2838..3632
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=Neo
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
terminator 4022..4274
/label=NOS
/note="nopaline synthase terminator and poly(A) signal"
promoter 5461..5806
/label=35S
/note="strong constitutive promoter from cauliflower mosaic
virus"
protein_bind 5828..5952
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 5989..6019
/label=lac UV5
/note="E. coli lac promoter with an ""up"" mutation"
CDS 6073..6732
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=Chl
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
CDS 7074..7379
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
protein_bind complement(7420..7544)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
CDS 7555..7578
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
terminator 7600..7852
/label=NOS
/note="nopaline synthase terminator and poly(A) signal"
terminator 8281..8533
/label=NOS
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(9001..10026)
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=Hyg
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
promoter complement(10036..10380)
/label=35S
/note="strong constitutive promoter from cauliflower mosaic
virus"
misc_feature 11124..11148
/label=LB
/note="left border repeat from nopaline C58 T-DNA"
CDS complement(12744..13892)
/codon_start=1
/product="trans-acting replication protein that binds to
and activates oriV"
/label=trfA
/translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
CDS complement(14191..14985)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=Kan
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
mobile_element 15177..15944
/mobile_element_type="insertion sequence:IS1"
/note="prokaryotic transposable element"
rep_origin 16567..18
/label=oriV
/note="incP origin of replication"
ORIGIN
1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag
4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
5701 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
5761 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
5821 gttatcaaca agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa
5881 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat
5941 ccagtcacta tggcggcccc tcgaggggcc gcattaggca ccccaggctt tacactttat
6001 gcttccggct cgtataatgt gtggattttg agttaggatc cggcgagatt ttcaggagct
6061 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
6121 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
6181 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
6241 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
6301 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
6361 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
6421 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
6481 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
6541 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
6601 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt
6661 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
6721 ggcggggcgt aaagatctgg atccggctta ctaaaagcca gataacagta tgcgtatttg
6781 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa
6841 agaggtgtgc tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc
6901 tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc
6961 ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc
7021 ccggtttatt gaaatgaacg gctcttttgc tgacgagaac agggactggt gaaatgcagt
7081 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
7141 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
7201 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
7261 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
7321 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
7381 tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt
7441 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
7501 atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttgataa cagcgactac
7561 aaggatgacg atgacaaggc ttagagctcg aatttccccg atcgttcaaa catttggcaa
7621 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
7681 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
7741 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
7801 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta
7861 gcttcatcaa cgcaagacat gcgcacgacc gtctgacagg agaggaattt ccgacgagca
7921 cagaaaggac ttgctcttgg acgtaggcct atttctcagg cacatgtatc aagtgttcgg
7981 acgtgggttt tcgatggtgt atcagccgcc gccaactggg agatgaggag gctttcttgg
8041 ggggcagtca gcagttcatt tcacaagaca gaggaacttg taaggagatg cactgattta
8101 tcttggcgca aaccagcagg acgaattagt gggaatagcc cgcgaatatc taagttatgc
8161 ctgtcggcat gagcagaaac ttccaattcg aaacagtttg gagaggttgt ttttgggcat
8221 accttttgtt agtcagcctc tcgattgctc atcgtcatta cacagtaccg aagtttgatc
8281 gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt
8341 tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca
8401 taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt
8461 atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa
8521 tgtttgaacg atctgcttcg acgcactcct tctttactcc accatctcgt ccttattgaa
8581 aacgtgggta gcaccaaaac gaatcaagtc gctggaactg aagttaccaa tcacgctgga
8641 tgatttgcca gttggattaa tcttgccttt ccccgcatga ataatattga tgaatgcatg
8701 cgtgaggggt atttcgattt tggcaatagc tgcaattgcc gcgacatcct ccaacgagca
8761 taattcttca gaaaaatagc gatgttccat gttgtcaggg catgcatgat gcacgttatg
8821 aggtgacggt gctaggcagt attccctcaa agtttcatag tcagtatcat attcatcatt
8881 gcattcctgc aagagagaat tgagacgcaa tccacacgct gcggcaacct tccggcgttc
8941 gtggtctatt tgctcttgga cgttgcaaac gtaagtgttg gatcccggtc ggcatctact
9001 ctattccttt gccctcggac gagtgctggg gcgtcggttt ccactatcgg cgagtacttc
9061 tacacagcca tcggtccaga cggccgcgct tctgcgggcg atttgtgtac gcccgacagt
9121 cccggctccg gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa
9181 attgccgtca accaagctct gatagagttg gtcaagacca atgcggagca tatacgcccg
9241 gagccgcggc gatcctgcaa gctccggatg cctccgctcg aagtagcgcg tctgctgctc
9301 catacaagcc aaccacggcc tccagaagaa gatgttggcg acctcgtatt gggaatcccc
9361 gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg ccattgtccg tcaggacatt
9421 gttggagccg aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct cggcccaaag
9481 catcagctca tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg
9541 ccagtgatac acatggggat cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg
9601 accgattcct tgcggtccga atgggccgaa cccgctcgtc tggctaagat cggccgcagc
9661 gatcgcatcc atggcctccg cgaccggctg cagaacagcg ggcagttcgg tttcaggcag
9721 gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc tctcgctgaa
9781 ttccccaatg tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac
9841 ataacgatct ttgtagaaac catcggcgca gctatttacc cgcaggacat atccacgccc
9901 tcctacatcg aagctgaaag cacgagattc ttcgccctcc gagagctgca tcaggtcgga
9961 gacgctgtcg aacttttcga tcagaaactt ctcgacagac gtcgcggtga gttcaggctt
10021 tttcatatcg gggtcgtcct ctccaaatga aatgaacttc cttatataga ggaagggtct
10081 tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatatc acatcaatcc
10141 acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg
10201 ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg
10261 caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag
10321 atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca
10381 atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg
10441 tgctccacca tgttgacgga tctctaggac gcgtcctaga agctaattca ctggccgtcg
10501 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
10561 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
10621 agttgcgcag cctgaatggc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
10681 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
10741 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
10801 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
10861 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa
10921 gggattttgc cgatttcgga accaccatca aacaggattt tcgcctgctg gggcaaacca
10981 gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc
11041 ccgtctcact ggtgaaaaga aaaaccaccc cagtacatta aaaacgtccg caatgtgtta
11101 ttaagttgtc taagcgtcaa tttgtttaca ccacaatata tcctgccacc agccagccaa
11161 cagctccccg accggcagct cggcacaaaa tcaccactcg atacaggcag cccatcagtc
11221 cgggacggcg tcagcgggag agccgttgta aggcggcaga ctttgctcat gttaccgatg
11281 ctattcggaa gaacggcaac taagctgccg ggtttgaaac acggatgatc tcgcggaggg
11341 tagcatgttg attgtaacga tgacagagcg ttgctgcctg tgatcaaata tcatctccct
11401 cgcagagatc cgaattatca gccttcttat tcatttctcg cttaaccgtg acaggctgtc
11461 gatcttgaga actatgccga cataatagga aatcgctgga taaagccgct gaggaagctg
11521 agtggcgcta tttctttaga agtgaacgtt gacgatatca actcccctat ccattgctca
11581 ccgaatggta caggtcgggg acccgaagtt ccgactgtcg gcctgatgca tccccggctg
11641 atcgacccca gatctggggc tgagaaagcc cagtaaggaa acaactgtag gttcgagtcg
11701 cgagatcccc cggaaccaaa ggaagtaggt taaacccgct ccgatcaggc cgagccacgc
11761 caggccgaga acattggttc ctgtaggcat cgggattggc ggatcaaaca ctaaagctac
11821 tggaacgagc agaagtcctc cggccgccag ttgccaggcg gtaaaggtga gcagaggcac
11881 gggaggttgc cacttgcggg tcagcacggt tccgaacgcc atggaaaccg cccccgccag
11941 gcccgctgcg acgccgacag gatctagcgc tgcgtttggt gtcaacacca acagcgccac
12001 gcccgcagtt ccgcaaatag cccccaggac cgccatcaat cgtatcgggc tacctagcag
12061 agcggcagag atgaacacga ccatcagcgg ctgcacagcg cctaccgtcg ccgcgacccc
12121 gcccggcagg cggtagaccg aaataaacaa caagctccag aatagcgaaa tattaagtgc
12181 gccgaggatg aagatgcgca tccaccagat tcccgttgga atctgtcgga cgatcatcac
12241 gagcaataaa cccgccggca acgcccgcag cagcataccg gcgacccctc ggcctcgctg
12301 ttcgggctcc acgaaaacgc cggacagatg cgccttgtga gcgtccttgg ggccgtcctc
12361 ctgtttgaag accgacagcc caatgatctc gccgtcgatg taggcgccga atgccacggc
12421 atctcgcaac cgttcagcga acgcctccat gggctttttc tcctcgtgct cgtaaacgga
12481 cccgaacatc tctggagctt tcttcagggc cgacaatcgg atctcgcgga aatcctgcac
12541 gtcggccgct ccaagccgtc gaatctgagc cttaatcaca attgtcaatt ttaatcctct
12601 gtttatcggc agttcgtaga gcgcgccgtg cgtcccgagc gatactgagc gaagcaagtg
12661 cgtcgagcag tgcccgcttg ttcctgaaat gccagtaaag cgctggctgc tgaaccccca
12721 gccggaactg accccacaag gccctagcgt ttgcaatgca ccaggtcatc attgacccag
12781 gcgtgttcca ccaggccgct gcctcgcaac tcttcgcagg cttcgccgac ctgctcgcgc
12841 cacttcttca cgcgggtgga atccgatccg cacatgaggc ggaaggtttc cagcttgagc
12901 gggtacggct cccggtgcga gctgaaatag tcgaacatcc gtcgggccgt cggcgacagc
12961 ttgcggtact tctcccatat gaatttcgtg tagtggtcgc cagcaaacag cacgacgatt
13021 tcctcgtcga tcaggacctg gcaacgggac gttttcttgc cacggtccag gacgcggaag
13081 cggtgcagca gcgacaccga ttccaggtgc ccaacgcggt cggacgtgaa gcccatcgcc
13141 gtcgcctgta ggcgcgacag gcattcctcg gccttcgtgt aataccggcc attgatcgac
13201 cagcccaggt cctggcaaag ctcgtagaac gtgaaggtga tcggctcgcc gataggggtg
13261 cgcttcgcgt actccaacac ctgctgccac accagttcgt catcgtcggc ccgcagctcg
13321 acgccggtgt aggtgatctt cacgtccttg ttgacgtgga aaatgacctt gttttgcagc
13381 gcctcgcgcg ggattttctt gttgcgcgtg gtgaacaggg cagagcgggc cgtgtcgttt
13441 ggcatcgctc gcatcgtgtc cggccacggc gcaatatcga acaaggaaag ctgcatttcc
13501 ttgatctgct gcttcgtgtg tttcagcaac gcggcctgct tggcctcgct gacctgtttt
13561 gccaggtcct cgccggcggt ttttcgcttc ttggtcgtca tagttcctcg cgtgtcgatg
13621 gtcatcgact tcgccaaacc tgccgcctcc tgttcgagac gacgcgaacg ctccacggcg
13681 gccgatggcg cgggcagggc agggggagcc agttgcacgc tgtcgcgctc gatcttggcc
13741 gtagcttgct ggaccatcga gccgacggac tggaaggttt cgcggggcgc acgcatgacg
13801 gtgcggcttg cgatggtttc ggcatcctcg gcggaaaacc ccgcgtcgat cagttcttgc
13861 ctgtatgcct tccggtcaaa cgtccgattc attcaccctc cttgcgggat tgccccgact
13921 cacgccgggg caatgtgccc ttattcctga tttgacccgc ctggtgcctt ggtgtccaga
13981 taatccacct tatcggcaat gaagtcggtc ccgtagaccg tctggccgtc cttctcgtac
14041 ttggtattcc gaatcttgcc ctgcacgaat accagcgacc ccttgcccaa atacttgccg
14101 tgggcctcgg cctgagagcc aaaacacttg atgcggaaga agtcggtgcg ctcctgcttg
14161 tcgccggcat cgttgcgcca catctaggta ctaaaacaat tcatccagta aaatataata
14221 ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct cgacatactg
14281 ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat accacttgtc
14341 cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt tcacaaagat
14401 gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt
14461 taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc agttttcgca
14521 atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc ggctgtctaa
14581 gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga tgcactccgc
14641 atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg agcaaaggac
14701 gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa agtgcaggac
14761 ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc gttccacatc
14821 ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt cattttctcc
14881 caccagctta tataccttag caggagacat tccttccgta tcttttacgc agcggtattt
14941 ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt atttccttcc
15001 tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac gaactccaat
15061 tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt
15121 tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccaca attatgggtg
15181 atgctgccaa cttactgatt tagtgtatga tggtgttttt gaggtgctcc agtggcttct
15241 gtgtctatca gctgtccctc ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc
15301 accgccggac atcagcgcta tctctgctct cactgccgta aaacatggca actgcagttc
15361 acttacaccg cttctcaacc cggtacgcac cagaaaatca ttgatatggc catgaatggc
15421 gttggatgcc gggcaacagc ccgcattatg ggcgttggcc tcaacacgat tttacgtcac
15481 ttaaaaaact caggccgcag tcggtaacct cgcgcataca gccgggcagt gacgtcatcg
15541 tctgcgcgga aatggacgaa cagtggggct atgtcggggc taaatcgcgc cagcgctggc
15601 tgttttacgc gtatgacagt ctccggaaga cggttgttgc gcacgtattc ggtgaacgca
15661 ctatggcgac gctggggcgt cttatgagcc tgctgtcacc ctttgacgtg gtgatatgga
15721 tgacggatgg ctggccgctg tatgaatccc gcctgaaggg aaagctgcac gtaatcagca
15781 agcgatatac gcagcgaatt gagcggcata acctgaatct gaggcagcac ctggcacggc
15841 tgggacggaa gtcgctgtcg ttctcaaaat cggtggagct gcatgacaaa gtcatcgggc
15901 attatctgaa cataaaacac tatcaataag ttggagtcat tacccaatta tgatagaatt
15961 tacaagctat aaggttattg tcctgggttt caagcattag tccatgcaag tttttatgct
16021 ttgcccattc tatagatata ttgataagcg cgctgcctat gccttgcccc ctgaaatcct
16081 tacatacggc gatatcttct atataaaaga tatattatct tatcagtatt gtcaatatat
16141 tcaaggcaat ctgcctcctc atcctcttca tcctcttcgt cttggtagct ttttaaatat
16201 ggcgcttcat agagtaattc tgtaaaggtc caattctcgt tttcatacct cggtataatc
16261 ttacctatca cctcaaatgg ttcgctgggt ttatcgcacc cccgaacacg agcacggcac
16321 ccgcgaccac tatgccaaga atgcccaagg taaaaattgc cggccccgcc atgaagtccg
16381 tgaatgcccc gacggccgaa gtgaagggca ggccgccacc caggccgccg ccctcactgc
16441 ccggcacctg gtcgctgaat gtcgatgcca gcacctgcgg cacgtcaatg cttccgggcg
16501 tcgcgctcgg gctgatcgcc catcccgtta ctgccccgat cccggcaatg gcaaggactg
16561 ccagcgctgc catttttggg gtgaggccgt tcgcggccga ggggcgcagc ccctgggggg
16621 atgggaggcc cgcgttagcg ggccgggagg gttcgagaag ggggggcacc ccccttcggc
16681 gtgcgcggtc acgcgcacag ggcgcagccc tggttaaaaa caaggtttat aaatattggt
16741 ttaaaagcag gttaaaagac aggttagcgg tggccgaaaa acgggcggaa acccttgcaa
16801 atgctggatt ttctgcctgt ggacagcccc tcaaatgtca ataggtgcgc ccctcatctg
16861 tcagcactct gcccctcaag tgtcaaggat cgcgcccctc atctgtcagt agtcgcgccc
16921 ctcaagtgtc aataccgcag ggcacttatc cccaggcttg tccacatcat ctgtgggaaa
16981 ctcgcgtaaa atcaggcgtt ttcgccgatt tgcgaggctg gccagctcca cgtcgccggc
17041 cgaaatcgag cctgcccctc atctgtcaac gccgcgccgg gtgagtcggc ccctcaagtg
17101 tcaacgtccg cccctcatct gtcagtgagg gccaagtttt ccgcgaggta tccacaacgc
17161 cggcggccgc ggtgtctcgc acacggcttc gacggcgttt ctggcgcgtt tgcagggcca
17221 tagacggccg ccagcccagc ggcgagggca accagcccgg
//
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