Catalog No. PVT11309
Packing 2ug
pHCMC04 is a Bacillus subtilis xylose inducible expression plasmid.
LOCUS Exported 8089 bp ds-DNA circular SYN 06-JAN-2018
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..8089
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(440..1028)
/direction=LEFT
/label=ori
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1199..2059)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2060..2164)
/gene="bla"
/label=AmpR promoter
CDS complement(2343..2993)
/codon_start=1
/label=Chl
/translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
CDS complement(3497..4621)
/codon_start=1
/label=xylR
/translation="MNQKLILDEILKNSPVSRATLSEITGLNKSTVSSQVNTLLEKDFI
FEIGAGQSRGGRRPVMLVFNKNAGYSIGIDIGVDYLNGILTDLEGNIILEKTSDLSSSS
ASEVKEILFALIHGFVTHMPESPYGLVGIGICVPGLVDRHQQIIFMPNLNWNIKDLQFL
IESEFNVPVFVENEANAGAYGEKVFGMTKNYENIVYISINIGIGTGLVINNELYKGVQG
FSGEMGHMTIDFNGPKCSCGNRGCWELYASEKALLASLSKEEKNISRKEIVERANKNDV
EMLNALQNFGFYIGIGLTNILNTFDIEAVILRNHIIESHPIVLNTIKNEVSSRVHSHLD
NKCELLPSSLGKNAPALGAVSIVIDSFLSVTPIS"
misc_feature 4729..4882
/label=xylA promoter
misc_feature 4805..4829
/label=xylO
misc_feature 4898..4997
/label=MCS
ORIGIN
1 tcgaggcctg caggcatgca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
61 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
121 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
181 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
241 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
301 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
361 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
421 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
481 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
541 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
601 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
661 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
721 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
781 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
841 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
901 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
961 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
1021 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
1081 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
1141 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
1201 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
1261 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
1321 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
1381 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
1441 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
1501 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
1561 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
1621 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
1681 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
1741 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
1801 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
1861 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
1921 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
1981 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
2041 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
2101 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
2161 cgcgcacatt tccccgaaaa gtgccacctg actgccgggc ctcttgcggg atcggcgagg
2221 ctagttaccc ttaagttatt ggtatgactg gttttaagcg caaaaaaagt tgctttttcg
2281 tacctattaa tgtatcgttt tagaaaaccg actgtaaaaa gtacagtcgg cattatctca
2341 tattataaaa gccagtcatt aggcctatct gacaattcct gaatagagtt cataaacaat
2401 cctgcatgat aaccatcaca aacagaatga tgtacctgta aagatagcgg taaatatatt
2461 gaattacctt tattaatgaa ttttcctgct gtaataatgg gtagaaggta attactatta
2521 ttattgatat ttaagttaaa cccagtaaat gaagtccatg gaataataga aagagaaaaa
2581 gcattttcag gtataggtgt tttgggaaac aatttccccg aaccattata tttctctaca
2641 tcagaaaggt ataaatcata aaactctttg aagtcattct ttacaggagt ccaaatacca
2701 gagaatgttt tagatacacc atcaaaaatt gtataaagtg gctctaactt atcccaataa
2761 cctaactctc cgtcgctatt gtaaccagtt ctaaaagctg tatttgagtt tatcaccctt
2821 gtcactaaga aaataaatgc agggtaaaat ttatatcctt cttgttttat gtttcggtat
2881 aaaacactaa tatcaatttc tgtggttata ctaaaagtcg tttgttggtt caaataatga
2941 ttaaatatct cttttctctt ccaattgtct aaatcaattt tattaaagtt catttgatat
3001 gcctcctaaa tttttatcta aagtgaattt aggaggctta cttgtctgct ttcttcatta
3061 gaatcaatcc ttttttaaaa gtcaatatta ctgtaacata aatatatatt ttaaaaatat
3121 cccactttat ccaattttcg tttgttgaac taatgggtgc tttagttgaa gaataaagac
3181 cacattaaaa aatgtggtct tttgtgtttt tttaaaggat ttgagcgtag cgaaaaatcc
3241 ttttctttct tatcttgata ataagggtaa ctattgccga tcgtccattc cgacagcatc
3301 gccagtcact atggcgtgct gctagcgcca ttcgccattc aggctgcgca actgttggga
3361 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
3421 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
3481 cagtgaattc gagctcctaa cttatagggg taacacttaa aaaagaatca ataacgatag
3541 aaaccgctcc taaagcaggt gcattttttc ctaacgaaga aggcaatagt tcacatttat
3601 tgtctaaatg agaatggact ctagaagaaa cttcgttttt aatcgtattt aaaacaatgg
3661 gatgagattc aattatatga tttctcaaga taacagcttc tatatcaaat gtattaagga
3721 tattggttaa tccaattccg atataaaagc caaagttttg aagtgcattt aacatttcta
3781 catcattttt atttgcgcgt tccacaatct cttttcgaga aatattcttt tcttctttag
3841 agagcgaagc cagtaacgct ttttcagaag catataattc ccaacagcct cgatttccac
3901 agctgcattt gggtccatta aaatctatcg tcatatgacc catttcccca gaaaaaccct
3961 gaacaccttt atacaattcg ttgttaataa caagtccagt tccaattccg atattaatac
4021 tgatgtaaac gatgttttca tagttttttg tcataccaaa tactttttca ccgtatgctc
4081 ctgcattagc ttcattttca acaaaaaccg gaacattaaa ctcactctca attaaaaact
4141 gcaaatcttt gatattccaa tttaagttag gcatgaaaat aatttgctga tgacgatcta
4201 caaggcctgg aacacaaatt cctattccga ctagaccata aggggactca ggcatatggg
4261 ttacaaaacc atgaataagt gcaaataaaa tctcttttac ttcactagcg gaagaactag
4321 acaagtcaga agtcttctcg agaataatat ttccttctaa gtcggttaga attccgttaa
4381 gatagtcgac tcctatatca ataccaatcg agtagcctgc attcttatta aaaacaagca
4441 ttacaggtct tctgccgcct ctagattgcc ctgccccaat ttcaaaaata aaatcttttt
4501 caagcagtgt atttacttga gaggagacag tagacttgtt taatcctgta atctcagaga
4561 gagttgccct ggagacaggg gagttcttca aaatttcatc taatattaat ttttgattca
4621 ttttttttac taaagcttga tctgcaattt gaataataac cactcctttg tttatccacc
4681 gaactaagtt ggtgtttttt gaagcttgaa ttagatattt aaaagtatca tatctaatat
4741 tataactaaa ttttctaaaa aaaacattga aataaacatt tattttgtat atgatgagat
4801 aaagttagtt tattggataa acaaactaac tcaattaaga tagttgatgg ataaacttgt
4861 tcacttaaat caaaggggga aatgacaaat ggtccaaact agtgatatct aaaaatcaaa
4921 gggggaaatg ggatcctcta gagtcgacgt ccccggggca gcccgcctaa tgagcgggct
4981 tttttcacgt cacgcgtcca tggagatctt tgtctgcaac tgaaaagttt ataccttacc
5041 tggaacaaat ggttgaaaca tacgaggcta atatcggctt attaggaata gtccctgtac
5101 taataaaatc aggtggatca gttgatcagt atattttgga cgaagctcgg aaagaatttg
5161 gagatgactt gcttaattcc acaattaaat taagggaaag aataaagcga tttgatgttc
5221 aaggaatcac ggaagaagat actcatgata aagaagctct aaaactattc aataacctta
5281 caatggaatt gatcgaaagg gtggaaggtt aatggtacga aaattagggg atctacctag
5341 aaagccacaa ggcgataggt caagcttaaa gaacccttac atggatctta cagattctga
5401 aagtaaagaa acaacagagg ttaaacaaac agaaccaaaa agaaaaaaag cattgttgaa
5461 aacaatgaaa gttgatgttt caatccataa taagattaaa tcgctgcacg aaattctggc
5521 agcatccgaa gggaattcat attacttaga ggatactatt gagagagcta ttgataagat
5581 ggttgagaca ttacctgaga gccaaaaaac tttttatgaa tatgaattaa aaaaaagaac
5641 caacaaaggc tgagacagac tccaaacgag tctgtttttt taaaaaaaat attaggagca
5701 ttgaatatat attagagaat taagaaagac atgggaataa aaatatttta aatccagtaa
5761 aaatatgata agattatttc agaatatgaa gaactctgtt tgtttttgat gaaaaaacaa
5821 acaaaaaaaa tccacctaac ggaatctcaa tttaactaac agcggccaaa ctgagaagtt
5881 aaatttgaga aggggaaaag gcggatttat acttgtattt aactatctcc attttaacat
5941 tttattaaac cccatacaag tgaaaatcct cttttacact gttcctttag gtgatcgcgg
6001 agggacatta tgagtgaagt aaacctaaaa ggaaatacag atgaattagt gtattatcga
6061 cagcaaacca ctggaaataa aatcgccagg aagagaatca aaaaagggaa agaagaagtt
6121 tattatgttg ctgaaacgga agagaagata tggacagaag agcaaataaa aaacttttct
6181 ttagacaaat ttggtacgca tataccttac atagaaggtc attatacaat cttaaataat
6241 tacttctttg atttttgggg ctatttttta ggtgctgaag gaattgcgct ctatgctcac
6301 ctaactcgtt atgcatacgg cagcaaagac ttttgctttc ctagtctaca aacaatcgct
6361 aaaaaaatgg acaagactcc tgttacagtt agaggctact tgaaactgct tgaaaggtac
6421 ggttttattt ggaaggtaaa cgtccgtaat aaaaccaagg ataacacaga ggaatccccg
6481 atttttaaga ttagacgtaa ggttcctttg ctttcagaag aacttttaaa tggaaaccct
6541 aatattgaaa ttccagatga cgaggaagca catgtaaaga aggctttaaa aaaggaaaaa
6601 gagggtcttc caaaggtttt gaaaaaagag cacgatgaat ttgttaaaaa aatgatggat
6661 gagtcagaaa caattaatat tccagaggcc ttacaatatg acacaatgta tgaagatata
6721 ctcagtaaag gagaaattcg aaaagaaatc aaaaaacaaa tacctaatcc tacaacatct
6781 tttgagagta tatcaatgac aactgaagag gaaaaagtcg acagtacttt aaaaagcgaa
6841 atgcaaaatc gtgtctctaa gccttctttt gatacctggt ttaaaaacac taagatcaaa
6901 attgaaaata aaaattgttt attacttgta ccgagtgaat ttgcatttga atggattaag
6961 aaaagatatt tagaaacaat taaaacagtc cttgaagaag ctggatatgt tttcgaaaaa
7021 atcgaactaa gaaaagtgca ataaactgct gaagtatttc agcagttttt tttatttaga
7081 aatagtgaaa aaaatataat cagggaggta tcaatattta atgagtactg atttaaattt
7141 atttagactg gaattaataa ttaacacgta gactaattaa aatttaatga gggataaaga
7201 ggatacaaaa atattaattt caatccctat taaattttaa caaggggggg attaaaattt
7261 aattagaggt ttatccacaa gaaaagaccc taataaaatt tttactaggg ttataacact
7321 gattaatttc ttaatggggg agggattaaa atttaatgac aaagaaaaca atcttttaag
7381 aaaagctttt aaaagataat aataaaaaga gctttgcgat taagcaaaac tctttacttt
7441 ttcattgaca ttatcaaatt catcgatttc aaattgttgt tgtatcataa agttaattct
7501 gttttgcaca accttttcag gaatataaaa cacatctgag gcttgtttta taaactcagg
7561 gtcgctaaag tcaatgtaac gtagcatatg atatggtata gcttccaccc aagttagcct
7621 ttctgcttct tctgaatgtt tttcatatac ttccatgggt atctctaaat gattttcctc
7681 atgtagcaag gtatgagcaa aaagtttatg gaattgatag ttcctctctt tttcttcaac
7741 ttttttatct aaaacaaaca ctttaacatc tgagtcaatg taagcataag atgtttttcc
7801 agtcataatt tcaatcccaa atcttttaga cagaaattct ggacgtaaat cttttggtga
7861 aagaattttt ttatgtagca atatatccga tacagcacct tctaaaagcg ttggtgaata
7921 gggcatttta cctatctcct ctcattttgt ggaataaaaa tagtcatatt cgtccatcta
7981 cctatcctat tatcgaacag ttgaactttt taatcaagga tcagtccttt ttttcattat
8041 tcttaaactg tgctcttaac tttaacaact cgatttgttt ttccagatc
//
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3. Shipping temperature is 2-8℃.
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