pHCMC04

$260.00

  • Model: PVT11309
  • 50 Units in Stock
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pHCMC04

Catalog No. PVT11309
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pHCMC04 Information

Promoter: xylA promoter

Prokaryotic resistance: ampicillin Amp

Screening markers: chloramphenicol Chl

Cloned strain: Escherichia coli DH5 alpha

Culture conditions: 37 degrees centigrade

Expression host: Bacillus subtilis

Inducement: xylose

 

pHCMC04 Description

PHCMC04 is a Bacillus subtilis xylose inducible expression plasmid.

pHCMC04-vector

 

pHCMC04 Sequence

LOCUS       Exported                8089 bp ds-DNA     circular SYN 06-JAN-2018
DEFINITION  synthetic circular DNA
FEATURES             Location/Qualifiers
     source          1..8089
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(440..1028)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1199..2059)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2060..2164)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(2343..2993)
                     /codon_start=1
                     /label=Chl
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     CDS             complement(3497..4621)
                     /codon_start=1
                     /label=xylR
                     /translation="MNQKLILDEILKNSPVSRATLSEITGLNKSTVSSQVNTLLEKDFI
                     FEIGAGQSRGGRRPVMLVFNKNAGYSIGIDIGVDYLNGILTDLEGNIILEKTSDLSSSS
                     ASEVKEILFALIHGFVTHMPESPYGLVGIGICVPGLVDRHQQIIFMPNLNWNIKDLQFL
                     IESEFNVPVFVENEANAGAYGEKVFGMTKNYENIVYISINIGIGTGLVINNELYKGVQG
                     FSGEMGHMTIDFNGPKCSCGNRGCWELYASEKALLASLSKEEKNISRKEIVERANKNDV
                     EMLNALQNFGFYIGIGLTNILNTFDIEAVILRNHIIESHPIVLNTIKNEVSSRVHSHLD
                     NKCELLPSSLGKNAPALGAVSIVIDSFLSVTPIS"
     misc_feature    4729..4882
                     /label=xylA promoter
     misc_feature    4805..4829
                     /label=xylO
     misc_feature    4898..4997
                     /label=MCS
ORIGIN
        1 tcgaggcctg caggcatgca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
       61 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      121 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      181 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      241 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      301 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      361 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      421 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      481 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      541 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      601 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      661 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      721 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      781 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      841 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
      901 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      961 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1021 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1081 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1141 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     1201 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1261 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1321 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1381 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1441 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1501 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1561 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1621 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     1681 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     1741 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     1801 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     1861 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     1921 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     1981 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     2041 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     2101 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     2161 cgcgcacatt tccccgaaaa gtgccacctg actgccgggc ctcttgcggg atcggcgagg
     2221 ctagttaccc ttaagttatt ggtatgactg gttttaagcg caaaaaaagt tgctttttcg
     2281 tacctattaa tgtatcgttt tagaaaaccg actgtaaaaa gtacagtcgg cattatctca
     2341 tattataaaa gccagtcatt aggcctatct gacaattcct gaatagagtt cataaacaat
     2401 cctgcatgat aaccatcaca aacagaatga tgtacctgta aagatagcgg taaatatatt
     2461 gaattacctt tattaatgaa ttttcctgct gtaataatgg gtagaaggta attactatta
     2521 ttattgatat ttaagttaaa cccagtaaat gaagtccatg gaataataga aagagaaaaa
     2581 gcattttcag gtataggtgt tttgggaaac aatttccccg aaccattata tttctctaca
     2641 tcagaaaggt ataaatcata aaactctttg aagtcattct ttacaggagt ccaaatacca
     2701 gagaatgttt tagatacacc atcaaaaatt gtataaagtg gctctaactt atcccaataa
     2761 cctaactctc cgtcgctatt gtaaccagtt ctaaaagctg tatttgagtt tatcaccctt
     2821 gtcactaaga aaataaatgc agggtaaaat ttatatcctt cttgttttat gtttcggtat
     2881 aaaacactaa tatcaatttc tgtggttata ctaaaagtcg tttgttggtt caaataatga
     2941 ttaaatatct cttttctctt ccaattgtct aaatcaattt tattaaagtt catttgatat
     3001 gcctcctaaa tttttatcta aagtgaattt aggaggctta cttgtctgct ttcttcatta
     3061 gaatcaatcc ttttttaaaa gtcaatatta ctgtaacata aatatatatt ttaaaaatat
     3121 cccactttat ccaattttcg tttgttgaac taatgggtgc tttagttgaa gaataaagac
     3181 cacattaaaa aatgtggtct tttgtgtttt tttaaaggat ttgagcgtag cgaaaaatcc
     3241 ttttctttct tatcttgata ataagggtaa ctattgccga tcgtccattc cgacagcatc
     3301 gccagtcact atggcgtgct gctagcgcca ttcgccattc aggctgcgca actgttggga
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