pHelper Plasmid

$136.00

  • Model: PVT2101
  • 50 Units in Stock
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pHelper

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pHelper,Plasmid pHelper,pHelper vector

 

pHelper Information

Replicator: pUC ori, F1 ori

Plasmid classification: virus series, adenovirus associated packaging vector

Plasmid size: 11635bp

Prokaryotic resistance: Amp

Cloned strain: Stbl3

Culture conditions: 37 centigrade, aerobic, LB

Expression host: mammalian cells

Induction mode: no induction, instantaneous expression

5'sequencing primers: primers designed according to sequence

3'sequencing primers: primers designed according to sequence

 

pHelper Description

pHelper is a vector of 11.6 KB, carries the adenovirus genes (E2A E4 and VA RNAs required inducing the lytic) for phase of AAV. This vector is cotransfected into the packaging cell line to produce recombinant replication-deficient AAV virions., it is related to packaging adenovirus vector with ampicillin resistance.

 

pHelper Sequence

LOCUS       Exported               11635 bp ds-DNA     circular SYN 27-AUG-2016
DEFINITION  synthetic circular DNA
KEYWORDS    Untitled 7
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11635)
  AUTHORS   .
  TITLE     Direct Submission
             
FEATURES             Location/Qualifiers
     source          1..11635
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(9423..10011)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10182..11042)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11043..11147)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      complement(11173..11627)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ggtacccaac tccatgctta acagtcccca ggtacagccc accctgcgtc gcaaccagga
       61 acagctctac agcttcctgg agcgccactc gccctacttc cgcagccaca gtgcgcagat
      121 taggagcgcc acttcttttt gtcacttgaa aaacatgtaa aaataatgta ctaggagaca
      181 ctttcaataa aggcaaatgt ttttatttgt acactctcgg gtgattattt accccccacc
      241 cttgccgtct gcgccgttta aaaatcaaag gggttctgcc gcgcatcgct atgcgccact
      301 ggcagggaca cgttgcgata ctggtgttta gtgctccact taaactcagg cacaaccatc
      361 cgcggcagct cggtgaagtt ttcactccac aggctgcgca ccatcaccaa cgcgtttagc
      421 aggtcgggcg ccgatatctt gaagtcgcag ttggggcctc cgccctgcgc gcgcgagttg
      481 cgatacacag ggttgcagca ctggaacact atcagcgccg ggtggtgcac gctggccagc
      541 acgctcttgt cggagatcag atccgcgtcc aggtcctccg cgttgctcag ggcgaacgga
      601 gtcaactttg gtagctgcct tcccaaaaag ggtgcatgcc caggctttga gttgcactcg
      661 caccgtagtg gcatcagaag gtgaccgtgc ccggtctggg cgttaggata cagcgcctgc
      721 atgaaagcct tgatctgctt aaaagccacc tgagcctttg cgccttcaga gaagaacatg
      781 ccgcaagact tgccggaaaa ctgattggcc ggacaggccg cgtcatgcac gcagcacctt
      841 gcgtcggtgt tggagatctg caccacattt cggccccacc ggttcttcac gatcttggcc
      901 ttgctagact gctccttcag cgcgcgctgc ccgttttcgc tcgtcacatc catttcaatc
      961 acgtgctcct tatttatcat aatgctcccg tgtagacact taagctcgcc ttcgatctca
     1021 gcgcagcggt gcagccacaa cgcgcagccc gtgggctcgt ggtgcttgta ggttacctct
     1081 gcaaacgact gcaggtacgc ctgcaggaat cgccccatca tcgtcacaaa ggtcttgttg
     1141 ctggtgaagg tcagctgcaa cccgcggtgc tcctcgttta gccaggtctt gcatacggcc
     1201 gccagagctt ccacttggtc aggcagtagc ttgaagtttg cctttagatc gttatccacg
     1261 tggtacttgt ccatcaacgc gcgcgcagcc tccatgccct tctcccacgc agacacgatc
     1321 ggcaggctca gcgggtttat caccgtgctt tcactttccg cttcactgga ctcttccttt
     1381 tcctcttgcg tccgcatacc ccgcgccact gggtcgtctt cattcagccg ccgcaccgtg
     1441 cgcttacctc ccttgccgtg cttgattagc accggtgggt tgctgaaacc caccatttgt
     1501 agcgccacat cttctctttc ttcctcgctg tccacgatca cctctgggga tggcgggcgc
     1561 tcgggcttgg gagaggggcg cttctttttc tttttggacg caatggccaa atccgccgtc
     1621 gaggtcgatg gccgcgggct gggtgtgcgc ggcaccagcg catcttgtga cgagtcttct
     1681 tcgtcctcgg actcgagacg ccgcctcagc cgcttttttg ggggcgcgcg gggaggcggc
     1741 ggcgacggcg acggggacga cacgtcctcc atggttggtg gacgtcgcgc cgcaccgcgt
     1801 ccgcgctcgg gggtggtttc gcgctgctcc tcttcccgac tggccatttc cttctcctat
     1861 aggcagaaaa agatcatgga gtcagtcgag aaggaggaca gcctaaccgc cccctttgag
     1921 ttcgccacca ccgcctccac cgatgccgcc aacgcgccta ccaccttccc cgtcgaggca
     1981 cccccgcttg aggaggagga agtgattatc gagcaggacc caggttttgt aagcgaagac
     2041 gacgaggatc gctcagtacc aacagaggat aaaaagcaag accaggacga cgcagaggca
     2101 aacgaggaac aagtcgggcg gggggaccaa aggcatggcg actacctaga tgtgggagac
     2161 gacgtgctgt tgaagcatct gcagcgccag tgcgccatta tctgcgacgc gttgcaagag
     2221 cgcagcgatg tgcccctcgc catagcggat gtcagccttg cctacgaacg ccacctgttc
     2281 tcaccgcgcg taccccccaa acgccaagaa aacggcacat gcgagcccaa cccgcgcctc
     2341 aacttctacc ccgtatttgc cgtgccagag gtgcttgcca cctatcacat ctttttccaa
     2401 aactgcaaga tacccctatc ctgccgtgcc aaccgcagcc gagcggacaa gcagctggcc
     2461 ttgcggcagg gcgctgtcat acctgatatc gcctcgctcg acgaagtgcc aaaaatcttt
     2521 gagggtcttg gacgcgacga gaaacgcgcg gcaaacgctc tgcaacaaga aaacagcgaa
     2581 aatgaaagtc actgtggagt gctggtggaa cttgagggtg acaacgcgcg cctagccgtg
     2641 ctgaaacgca gcatcgaggt cacccacttt gcctacccgg cacttaacct accccccaag
     2701 gttatgagca cagtcatgag cgagctgatc gtgcgccgtg cacgacccct ggagagggat
     2761 gcaaacttgc aagaacaaac cgaggagggc ctacccgcag ttggcgatga gcagctggcg
     2821 cgctggcttg agacgcgcga gcctgccgac ttggaggagc gacgcaagct aatgatggcc
     2881 gcagtgcttg ttaccgtgga gcttgagtgc atgcagcggt tctttgctga cccggagatg
     2941 cagcgcaagc tagaggaaac gttgcactac acctttcgcc agggctacgt gcgccaggcc
     3001 tgcaaaattt ccaacgtgga gctctgcaac ctggtctcct accttggaat tttgcacgaa
     3061 aaccgcctcg ggcaaaacgt gcttcattcc acgctcaagg gcgaggcgcg ccgcgactac
     3121 gtccgcgact gcgtttactt atttctgtgc tacacctggc aaacggccat gggcgtgtgg
     3181 cagcaatgcc tggaggagcg caacctaaag gagctgcaga agctgctaaa gcaaaacttg
     3241 aaggacctat ggacggcctt caacgagcgc tccgtggccg cgcacctggc ggacattatc
     3301 ttccccgaac gcctgcttaa aaccctgcaa cagggtctgc cagacttcac cagtcaaagc
     3361 atgttgcaaa actttaggaa ctttatccta gagcgttcag gaattctgcc cgccacctgc
     3421 tgtgcgcttc ctagcgactt tgtgcccatt aagtaccgtg aatgccctcc gccgctttgg
     3481 ggtcactgct accttctgca gctagccaac taccttgcct accactccga catcatggaa
     3541 gacgtgagcg gtgacggcct actggagtgt cactgtcgct gcaacctatg caccccgcac
     3601 cgctccctgg tctgcaattc gcaactgctt agcgaaagtc aaattatcgg tacctttgag
     3661 ctgcagggtc cctcgcctga cgaaaagtcc gcggctccgg ggttgaaact cactccgggg
     3721 ctgtggacgt cggcttacct tcgcaaattt gtacctgagg actaccacgc ccacgagatt
     3781 aggttctacg aagaccaatc ccgcccgcca aatgcggagc ttaccgcctg cgtcattacc
     3841 cagggccaca tccttggcca attgcaagcc atcaacaaag cccgccaaga gtttctgcta
     3901 cgaaagggac ggggggttta cctggacccc cagtccggcg aggagctcaa cccaatcccc
     3961 ccgccgccgc agccctatca gcagccgcgg gcccttgctt cccaggatgg cacccaaaaa
     4021 gaagctgcag ctgcc
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