pHIV-H2BmRFP
Catalog No. PVT11082
Packing 2ug
Function /
Resistance Amp
Screen /
Strain Stbl3
Culture temperature 37degrees centigrade
Replicon pUC
Copy
Promoter PGK,CMV
Induction --
Forward primer CMV-F
Reverse primer
pHIV-H2BmRFP Information
Promoter: EF-1 alpha, CMV
Replicon: pUC ori
Plasmid classification: virus series, lentivirus cloning vector
Plasmid size: 8045bp
Prokaryotic resistance: Amp
Cloned strain: Stbl3
Culture conditions: 37 centigrade, aerobic LB
Expression host: mammalian cells
Induction mode: no induction, instantaneous expression
5'sequencing primers: CMV-F:CGCAAATGGGCGGTAGGCGTG
3'sequencing primers: primers designed according to sequence
pHIV-H2BmRFP Description
PHIV-H2BmRFP background vector HIV-Zsgreen, which is a high copy plasmid, is a slow virus vector, in the transformation of the cloned strain is Stbl3, at 37 C. The culture of the plasmid containing ampicillin.
pHIV-H2BmRFP Sequence
LOCUS Exported 8045 bp ds-DNA circular SYN 30-AUG-2016 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Untitled 6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8045) AUTHORS . TITLE Direct Submission JOURNAL Exported Tuesday, August 30, 2016 from SnapGene Viewer 3.1.4 FEATURES Location/Qualifiers source 1..8045 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 238..617 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" LTR 835..1015 /note="5' LTR (truncated)" /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /note="HIV-1 Psi" /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1686..1919 /note="RRE" /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." misc_feature 2446..2563 /note="cPPT/CTS" /note="central polypurine tract and central termination sequence of HIV-1" promoter 2627..3805 /note="EF-1-alpha promoter" /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2858..3796 /note="EF-1-alpha intron A" /note="intron upstream of the start codon of human EF-1-alpha" misc_feature 3851..4437 /note="IRES2" /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" CDS 4837..5514 /codon_start=1 /product="monomeric derivative of DsRed (Campbell et al., 2002)" /note="mRFP1" /note="mammalian codon-optimized" /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS TGA" primer_bind complement(5525..5541) /note="KS primer" /note="common sequencing primer, one of multiple similar variants" LTR 6051..6231 /note="5' LTR (truncated)" /note="truncated 5' long terminal repeat (LTR) from HIV-1" rep_origin complement(6293..6881) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7052..7912) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(7913..8017) /gene="bla" /note="AmpR promoter" ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga 1561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca 1621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg 1681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg 1741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac 1801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc 1861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg 1921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt 1981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga 2041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa 2101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt 2161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta 2221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca 2281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata 2341 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca 2401 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg 2461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca 2521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga 2581 cagcagagat ccagtttggt tagtaccggg cccgctctag ccgtgaggct ccggtgcccg 2641 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa 2701 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg 2761 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa 2821 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg 2881 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct 2941 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc 3001 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg 3061 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct 3121 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt 3181 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg 3241 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag 3301 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc 3361 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga 3421 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg 3481 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc 3541 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt 3601 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac 3661 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt 3721 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt 3781 ttttcttcca tttcaggtgt cgtgagcggc cgctgagtta actattctag agtacccggg 3841 ctaggatccg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat 3901 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg 3961 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc 4021 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt 4081 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg 4141 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac 4201 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg 4261 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg 4321 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc 4381 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg 4441 tcaccagagc cagcgaagtc tgctcccgcc ccgaaaaagg gctccaagaa ggcggtgact 4501 aaggcgcaga agaaaggcgg caagaagcgc aagcgcagcc gcaaggagag ctattccatc 4561 tatgtgtaca aggttctgaa gcaggtccac cctgacaccg gcatttcgtc caaggccatg 4621 ggcatcatga attcgtttgt gaacgacatt ttcgagcgca tcgcaggtga ggcttcccgc 4681 ctggcgcatt acaacaagcg ctcgaccatc acctccaggg agatccagac ggccgtgcgc 4741 ctgctgctgc ctggggagtt ggccaagcac gccgtgtccg agggtactaa ggccatcacc 4801 aagtacacca gcgctaagga tccaccggtc gccaccatgg cctcctccga ggacgtcatc 4861 aaggagttca tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca cgagttcgag 4921 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg 4981 accaagggcg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt ccagtacggc 5041 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc 5101 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc 5161 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac 5221 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag 5281 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agatgaggct gaagctgaag 5341 gacggcggcc actacgacgc cgaggtcaag accacctaca tggccaagaa gcccgtgcag 5401 ctgcccggcg cctacaagac cgacatcaag ctggacatca cctcccacaa cgaggactac 5461 accatcgtgg aacagtacga gcgcgccgag ggccgccact ccaccggcgc ctaagcggcc 5521 gcatcgatac cgtcgacctc gatcgagacc tagaaaaaca tggagcaatc acaagtagca 5581 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg 5641 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc 5701 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac 5761 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact 5821 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac 5881 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc 5941 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca 6001 gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct 6061 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 6121 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 6181 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agcatgtgag 6241 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 6301 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 6361 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 6421 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 6481 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 6541 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 6601 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 6661 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 6721 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 6781 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 6841 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 6901 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 6961 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 7021 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 7081 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 7141 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 7201 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgca
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