pHT304 Plasmid

$216.00

  • Model: PVT5009
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pHT304

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pHT304,Plasmid pHT304,pHT304 vector

pHT304 information

Promoter: Lac
Replicon: ColE1 ori
Plasmid classification: wide host series, Bacillus subtilis vector
Plasmid size: 6530bp
Plasmid Tags: LacZa
Prokaryotic resistance: Amp
Selection marker: ERM
Clone strain: DH5 alpha
Culture conditions: 37 LB, aerobic
Host: Bacillus subtilis
Induction method: IPTG induction
Primers for 5'sequencing: M13R:CAGGAAACAGCTATGACC
Primers for 3'sequencing: M13F:TGTAAAACGACGGCCAGT
Use: Bacillus subtilis
pHT304-plasmid

pHT304 description
The promoter of pHT304 vector is Lac, which can be used to induce the expression of recombinant protein in Escherichia coli DE3 engineering bacteria (DE3 BL21 or DE3 Rosetta). The tag has LacZa, which can be purified by nickel column.

pHT304 sequence
 

LOCUS       Exported File           6530 bp ds-DNA    circular SYN 29-9-2015
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    Untitled
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2015-9-29 
          
FEATURES             Location/Qualifiers
     source          1..6530
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             3862..4722
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4893..5481
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5769..5790
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5805..5835
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5843..5859
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5867..5883
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5879..6202
                     /codon_start=1
                     /gene="lacZ?fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /note="lacZ-alpha"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    5896..5952
                     /gene="
                     "
                     /note="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(5953..5969)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ccatcctcca aagttggaga gtgagtttta tgtcgcaaat attaatgttt ctggtgaacc
       61 ttatcaaatt ttcgttgatt taatagaaac atagcggtaa aattagcagt aacttaatag
      121 aacggaaatg aaaaaagcca ctctcatatg ctattggcta ccaaccttta gcgagaatga
      181 cttaatcctg tacagccata caggacttcg acttataaga ggcgccaacc tcaaataagt
      241 tatttgcctt gttttcgcga acaaggctta ttagatacac ctattgtacc gttactctac
      301 gaatatttca actagtaatt actagcattg tcatatacat aataaaacgg atataaaagg
      361 gcgttttcta tacctagaag tcttgtaaat gtacagggcg tttagatata gagaacgccc
      421 tttttgtgtt ccgttccagt ggaggctacc actttaaaaa gatggtctag tgtagccaat
      481 gcaggagagt acactcggat atcagttgtc gttgcattca actgtctgac gtaagcgagg
      541 taaaggacac aagccttgca taaaacaagc ctacgggatg taaatcctaa taatgatgat
      601 aaccaagacg ttagcggcaa aaagtgttgg gggttcaaaa taagacatga ttgtgcgact
      661 ggagttaaac agttactcgt aagcggcgat catgacactg attcacggct attcttgtac
      721 aaactttatt acaaggatat gcgggttata tagcgaatca cccgaaaggg aacggtgttg
      781 ggcgtgagaa acgcaccgta cggcgcaata caatgccaat aagctatata cggacggtat
      841 agtagttttg taagctataa ccgtttgtcg tcaatgcaac caatctcaat tcgagacctc
      901 ggcatctaag ccagtacgaa tgagtgggcg ttttaacctc gtaaattttc aacaggggtt
      961 actatgccca aaactacatt cagatttcct aacaaactcg ccagtatgaa aaccttaaga
     1021 ccttaaagtc aagggatttg aaggatttta acctcgatta gcaaaaaatg tagagtactg
     1081 aagcaactac cattaactaa gatagtgggg gattgaggaa gaatccagag ctgtttaaat
     1141 caagtgaaag acaagatgaa attaaaagaa tagtgaaaga taggggagtg gttctctatg
     1201 agaaaggaaa tggctagaga acaaaggcag cggtttattg atctattgtt agactttatg
     1261 gtaaagaatc ctcatttatt tgttaatggt acagaggatg aaagtaataa tgttgttaca
     1321 aaatgtaata gtgatattaa agaggttgcg gagtcatatt taactctttt atagtgagag
     1381 ggttaaaact aattaatatg tattaaggcc caatgttgga attattgtat ttcactaggc
     1441 aacctactta ctaaaagtaa gattatccat tagtggatgt tataatattg ggttttttaa
     1501 cacaataatc atcgcctttc ggtgtcgttt gatagaaaag taaccattag cgatgaaaaa
     1561 gtcaatataa aaagccatcc gtaaaaaacg gatggcttac cgtacatagg atcgttggta
     1621 gggcggcgta tcctacatct ctggtaactg acctagccaa tcaaatgctt gagaacggcg
     1681 gttagataag cgcgtgggga acctttccca cctcaaagat cctatatcat tattatgtta
     1741 ctttctacag gtagtatacc atgttcttat attttagtaa actccccgtt agcttaacag
     1801 gtctttgtaa gcaattaaac gtccactatt caatcgtctt tggattttcg caggaccgtt
     1861 ttttagatcg aacatagttg ataagaacaa ataaccgctt gggtccaact ttatagcaat
     1921 tagtatatgg tcatttaaaa tctttaccaa ttcaacgcta ttaggttctt taggattttg
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