Catalog No. PVT10523
Recombineering in Mycobacterium tuberculosis.
LOCUS Exported 8812 bp ds-DNA circular SYN 15-AUG-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..8812
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 3617..5615
/label=Che9c
/note="Gene/Insert name Che9c genes 60-61
Species Mycobacteriophage
Insert Size (bp) 1998"
terminator 5680..5726
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS 5887..6702
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin 7034..7622
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cggaccgcta ccggacagcg aggtccgcgc catcgccaac agcatttggc gttggatcac
61 aaccaagtcg cgcatttggg cggacgggat cgtggtctac gaggccacac tcagtgcgcg
121 ccatgcggcc atctcgcgga agggcgcagc agcgcgcacg gcggcgagca cagttgcgcg
181 gcgcgcaaag tccgcgtcag ccatggaggc attgctatga gcgacggcta cagcgacggc
241 tacagcgacg gctacaactg gcagccgact gtccgcaaaa agcggcgcgt gaccgccgcc
301 gaaggcgctc gaatcaccgg actatccgaa cgccacgtcg tccggctcgt ggcgcaggaa
361 cgcagcgagt ggttcgccga gcaggctgca cgccgcgaac gcatccgcgc ctatcacgac
421 gacgagggcc actcttggcc gcaaacggcc aaacatttcg ggctgcatct ggacaccgtt
481 aagcgactcg gctatcgggc gaggaaagag cgtgcggcag aacaggaagc ggctcaaaag
541 gcccacaacg aagccgacaa tccaccgctg ttctaacgca attggggagc gggtgtcgcg
601 ggggttccgt ggggggttcc gttgcaacgg gtcggacagg taaaagtcct ggtagacgct
661 agttttctgg tttgggccat gcctgtctcg ttgcgtgttt cgttgcgtcc gttttgaata
721 ccagccagac gagacggggt tctacgaatc ttggtcgata ccaagccatt tccgctgaat
781 atcgtggagc tcaccgccag aatcggtggt tgtggtgatg tacgtggcga actccgttgt
841 agtgcttgtg gtggcatccg tggcgcggcc gcggtaccag atctttaaat ctagataaag
901 aagtgacgcg gtctcaagcg tcgagcgtcg tcagcgtgtc gaggatgtcg aagtcgtagc
961 cgtcggcgct ggcgatgtag acctgctggt cgaattgact gtcgcgcata cacatcgggc
1021 cccggggccc gtcgaacccg acatcgtgcg cggatgccat caggtccggt atctcggggg
1081 agtgggcccg ctggaagatg gcctcgagcg caacgagacc ctcgtaacag gattcggcca
1141 tcgcgttgag cggtggcgcg tcggcgccgt agcgggcgac gtagctgccc atcaggtcca
1201 tggcacccgc ggtggccagt gaactgaagt acgccgcggc gacatagagg ttttcggtgg
1261 agccggcgcc gctggccagc agcatgttct cctccatcag cgggctgaac cgcgccatgc
1321 ggtcgtgccc gccggcgcgc gcgaactcgc ggttgaacaa cacggcgtcc tggccgacga
1381 gcagcatcaa cacggcctgc gcccccgacg cgatggcctt gcggaccggt gcgcggaaat
1441 cgtcggtgcc gtacgggacg tagatctccc gtctgagctc gaggtccaga tctcggcagt
1501 acgcgcgggc ggccgcggcg gaacggcgcg gccagatgta gtcatcgccg accaggcacc
1561 aggaccggat gccgaagtgg tcgcgcagcc aggcgagcgc gggcgcgatc tggatctgcg
1621 gtgtctcgcc tgtgcagaac acgcccggtg tgcgttcacc gccctcgtac aacgaggtgt
1681 agacgtacgg gatgcggtcg cggaccaccg gggagatgcg gttgcgcacg gccgagatgt
1741 gccagccggt cacggcgtcg agaccgtgac ctcgcaaccg gtcggcgacg gtccgggcga
1801 cgtcgtcgcc gggccgtccg ccgtcgagca cctcgatggt gaccttgcgg ccctgcaggc
1861 cgcctcggtc gttgacctcc ttggccgcga gctcggccac ggcctcgcac gaaggcgcga
1921 agattcccgc tggcccttga agcggaatca ccagcccgac gcggaactca acctcgccgt
1981 cctgcactcc agatcaccgt cgatcccgtg tagtctgcgc ttcaaagctt tctagcagaa
2041 ataattcatt ctgaacagac cccgccgtcg acacgaggag acacccacca tggccgccgg
2101 acagcagcgc cgccccaacc tcctgctgcc gttggtgcgt ctgacccacc tcgcggagtc
2161 ggcgatcgaa cgcgtgctcg cggactcgtc gctcaagatc gaggactggc gcgtgctcga
2221 cgagttggcc ggacggcgca ccgtgcccat gagcgatctc gcgcaggcca cgctgatcac
2281 gggtccgact ctcaccagaa ccgtcgatcg ccttgtgtcg caagggatca tctaccggac
2341 tgccgatctg catgaccgcc ggcgggtgct cgtggcgttg accccgcggg ggcggacgct
2401 gcgcaaccgc ctggtggacg cggtagccga ggccgagtgt gcggcttttg aatcgtgcgg
2461 gctggacgtc gaccagttgc gcgaactcgt cgacaccacc tcgaatttga cttcgtaacc
2521 acccgcgccc ggccggcgtt cacccttgac ttttattttc atctggatat atttcgggtg
2581 aatggaaagg ggtgaccatg ccgacctaca cattccgttg ttcccactgc ggtcccttcg
2641 atctcacctg cgcgatctcc gagcgcgatg cggcggcgac ctgtccggag tgccggacgc
2701 cggcgcgccg ggtcttcggt tcggtagggc tgacgacatt caccgcggga catcaccgcg
2761 cattcgacgc ggcgtccgcg agcgccgaaa gtcccacggt ggtgaagtcg attcccgcag
2821 gcgcggaccg cccgcgggcc ccgcgccgca atcccggtct accgagtctg ccgaggtact
2881 agcgacatgg gtggcgtcgg gctcttctac gtgggtgcgg tgctcatcat cgacgggctg
2941 atgctgctgg gccgcatcag cccacgaggc gcaacaccgc tgaacttctt cgtcggcgga
3001 ctgcaggtgg tgacgcctac ggtgctgatc ctgcagtccg gcggagacgc ggccgtgatc
3061 ttcgcggcct ccgggctcta cctgttcggc ttcacctacc tgtgggtggc catcaacaac
3121 gtgaccgact gggacggaga aggtctcgga tggttctcgc tgttcgtcgc gatcgccgca
3181 ctcggctact cgtggcacgc gttcaccgcc gaggccgacc cggcgttcgg ggtgatctgg
3241 ctgctgtggg cagtgctgtg gttcatgctg ttcctgctgc tcggcctggg gcacgacgca
3301 ctggggcccg ccgtcgggtt cgtcgcggtg gccgaaggcg tgatcaccgc cgccgtgccg
3361 gccttcctga tcgtgtcggg caactgggaa accggcccgc tccccgccgc ggtcatcgcc
3421 gtgatcggtt ttgccgcagt tgttctcgca taccccatcg ggcgccgtct cgcagcgccg
3481 tcagtcacca accctccacc ggccgcgctc gcggccacca cccgataaga gaaagggagt
3541 ccacatatgt aacggatcca gctgcagaat tcgaagctta tcgatgtcga cgtagttacg
3601 agatcggcgg ccgcatatga gtgtgcccac acaggacgga atgcaccggt tcgtcgacga
3661 ggacgtctac cacgctgacc ggggctcgct gtcggtatcc ggcgcgaagc tgctgttgcc
3721 gccgtcgtgt cccgcgaaat tccgctggga gatggacaac acccggaagc cgaaaaaggt
3781 ctgggacttc ggacatgtcg cgcacaaact ggtgctcggc aagggtgccg agttcgagat
3841 cctcgacccc gaggtgcacg ggctgaaggc ggacggtacg ccgtcggaga agccgaccgc
3901 gacgggcatg tggcgcaagg ccgaggctga ggctcgcaaa cagggcaagg tgccgattca
3961 cgtcgacctg ttcacgaagg cgtacgacat ggccgaaaag gtgcgtcagc acccgacagc
4021 cggcccgatc ttcgccaatc ctgacggcga ggccgaggtc gcgctgtact acaccgaccc
4081 cgagaccggc gtgcggctgc gtggccggat cgactggctc actgacgata tcgatgatta
4141 caagacgtcg atgaccgcga acccggccga gctgaaaacc aagttctaca agctcggcta
4201 tttcatgcag gcggcctggt acatcgatct actggtcgcc ctcgggctcg ccgagaaccc
4261 gcgattccgg ttcatcacgc aggagaaaga accgccctac gtcgtgactc cgatccagta
4321 cgacgacgag gcgatcgaag aggggcggcg ccgcaaccgc caggcgatcc ggctctacgc
4381 cgactgcatg gaatcgggca agtggcctga ctacagcgac gacgtggtca cgatcagcct
4441 gccctcgtgg gggctgccgc gaccgcagac cgtcggcgac gtcgtcaccg acagctatat
4501 ctacgacacc gacccgctcg aagaggccga cccgattgaa ggggattaca tctatggctg
4561 aaaatgctgt caccaagcag gattcgccca aggcacccga gacgatctcg caagtgctgc
4621 aggtgctcgt gccgcagctg gcgcgtgcag tgcccaaggg tatggacccc gaccgcatcg
4681 cgcggatcgt gcaaaccgag atccgcaagt cgcgcaacgc gaaagctgct ggcatcgcta
4741 agcagtccct cgacgactgc acgcaagagt catttgccgg tgcgctgctg acctcggccg
4801 cgctcggtct cgagcccggt gtcaacggcg agtgctacct cgtgccctac cgcgacaccc
4861 ggcgcggcgt ggtcgagtgc cagctgatca tcggctacca gggcatcgtc aaactgttct
4921 ggcagcaccc gcgcgcctcc cggatcgacg cgcagtgggt cggcgcgaac gacgaattcc
4981 attacaccat gggcctcaat ccgacgctga aacacgtgaa ggccaagggt gatcggggta
5041 atccggtcta cttctacgcg atcgtcgagg tgaccggcgc tgagccgctg tgggacgtgt
5101 tcaccgccga cgagatcagg gaattgcgtc gcggcaaggt cggatcctcg ggcgacatca
5161 aggacccgca gcgctggatg gagcggaaga ccgcgctcaa acaggtgctg aagctggcac
5221 cgaagacgac gcggctcgac gcggcgatcc gcgccgacga tcgcccgggc accgacctgt
5281 cacagtcgca ggcgctcgcg ctgccgtcga ccgtcaagcc gacggccgac tacatcgacg
5341 gcgagatcgc cgagccgcac gaggtcgata cgccaccgaa gtcgtcgcgc gcacaacgcg
5401 cgcagcgcgc caccgcgccg gcgcccgacg tgcagatggc caatcccgat cagctgaagc
5461 gcctcggcga gatccagaag gccgaaaagt acaacgacgc cgattggttc aagttcctcg
5521 ccgattcggc cggcgtcaaa gccacgcgcg ccgccgacct cacgttcgac gaggcgaagg
5581 ctgtcatcga catgttcgac gggcccaacg catgagcgcc ccggcgaatt ccgacgcggt
5641 ggttgatctg caactagcgt acgatcgact gccaggcatc aaataaaacg aaaggctcag
5701 tcgaaagact gggcctttcg ttttatgcca tcatggccgc ggtgatcagc tagccacctg
5761 acgtcggggg ggggggaaag ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa
5821 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
5881 ggtgttatga gccatattca acgggaaacg tcttgctcga ggccgcgatt aaattccaac
5941 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
6001 acaatctatc gcttgtatgg gaagccccat gcgccagagt tgtttctgaa acatggcaaa
6061 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
6121 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
6181 actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
6241 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
6301 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
6361 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
6421 tggaaagaaa tgcataatct tttgccattc tcaccggatt cagtcgtcac tcatggtgat
6481 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
6541 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
6601 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
6661 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
6721 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcggctttgt tgaataaatc
6781 gaacttttgc tgagttgaag gatcagatca cgcatcttcc cgacaacgca gaccgttccg
6841 tggcaaagca aaagttcaaa atcaccaact ggtccaccta caacaaagct ctcatcaacc
6901 gtggctccct cactttctgg ctggatgatg gggcgattca ggcctggtat gagtcagcaa
6961 caccttcttc acgaggcaga cctcactagt tccactgagc gtcagacccc gtagaaaaga
7021 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
7081 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
7141 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
7201 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
7261 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
7321 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
7381 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
7441 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
7501 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
7561 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
7621 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
7681 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
7741 ctgataccgc tcgccgcagc cgaacgaccg agcgcaacgc gtgagcccac cagctccgta
7801 agttcgggtg ctgtgtggct cgtacccgcg cattcaggcg gcagggggtc taacgggtct
7861 aaggcggcgt gtacggccgc cacagcggct cttagcggcc cggaaacgtc ctcgaaacga
7921 cgcatgtgtt cctcctggtt ggtacaggtg gttgggggtg ctcggctgtc gctggtgttt
7981 catcatcagg gctcgacggg agagcggggg agtgtgcagt tgtggggtgg cccctcagcg
8041 aaatatctga cttggagctc gtgtcggacc atacaccggt gattaatcgt ggtttattat
8101 caagcgtgag ccacgtcgcc gacgaatttg agcagctctg gctgccgtac tggtccctgg
8161 caagcgacga tctgctcgag gggatctacc gccaaagccg cgcgtcggcc ctaggccgcc
8221 ggtacatcga ggcgaaccca acagcgctgg caaacctgct ggtcgtggac gtagaccatc
8281 cagacgcagc gctccgagcg ctcagcgccc gggggtccca tccgctgccc aacgcgatcg
8341 tgggcaatcg cgccaacggc cacgcacacg cagtgtgggc actcaacgcc cctgttccac
8401 gcaccgaata cgcgcggcgt aagccgctcg catacatggc ggcgtgcgcc gaaggccttc
8461 ggcgcgccgt cgatggcgac cgcagttact caggcctcat gaccaaaaac cccggccaca
8521 tcgcctggga aacggaatgg ctccactcag atctctacac actcagccac atcgaggccg
8581 agctcggcgc gaacatgcca ccgccgcgct ggcgtcagca gaccacgtac aaagcggctc
8641 cgacgccgct agggcggaat tgcgcactgt tcgattccgt caggttgtgg gcctatcttc
8701 ccgccctcat gcggatctac ctgccgaccc ggaacgtgga cggactcggc cgcgcgatct
8761 atgccgagtg ccacgcgcga aacgccgaat ttccgtgcaa cgacgtgtgt cc
//
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