pJV53
Catalog No. PVT10523
Packing 2ug
pJV53 Information
Function Other source plasmids
Plasmid size: 8812bp
Prokaryotic resistance: kanamycin Kan (50 mu g/ml)
Cloned strains of Escherichia coli, DH5 A and other Escherichia coli
Culture conditions: 37 centigrade, aerobic, LB
pJV53 Description
pJV53 plasmid provides the Che9c genes 60-61 gene of mycobacteriophage.
pJV53 References
Recombineering in Mycobacterium tuberculosis.
pJV53 Sequence
LOCUS Exported 8812 bp ds-DNA circular SYN 15-AUG-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..8812
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 3617..5615
/label=Che9c
/note="Gene/Insert name Che9c genes 60-61
Species Mycobacteriophage
Insert Size (bp) 1998"
terminator 5680..5726
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS 5887..6702
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin 7034..7622
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cggaccgcta ccggacagcg aggtccgcgc catcgccaac agcatttggc gttggatcac
61 aaccaagtcg cgcatttggg cggacgggat cgtggtctac gaggccacac tcagtgcgcg
121 ccatgcggcc atctcgcgga agggcgcagc agcgcgcacg gcggcgagca cagttgcgcg
181 gcgcgcaaag tccgcgtcag ccatggaggc attgctatga gcgacggcta cagcgacggc
241 tacagcgacg gctacaactg gcagccgact gtccgcaaaa agcggcgcgt gaccgccgcc
301 gaaggcgctc gaatcaccgg actatccgaa cgccacgtcg tccggctcgt ggcgcaggaa
361 cgcagcgagt ggttcgccga gcaggctgca cgccgcgaac gcatccgcgc ctatcacgac
421 gacgagggcc actcttggcc gcaaacggcc aaacatttcg ggctgcatct ggacaccgtt
481 aagcgactcg gctatcgggc gaggaaagag cgtgcggcag aacaggaagc ggctcaaaag
541 gcccacaacg aagccgacaa tccaccgctg ttctaacgca attggggagc gggtgtcgcg
601 ggggttccgt ggggggttcc gttgcaacgg gtcggacagg taaaagtcct ggtagacgct
661 agttttctgg tttgggccat gcctgtctcg ttgcgtgttt cgttgcgtcc gttttgaata
721 ccagccagac gagacggggt tctacgaatc ttggtcgata ccaagccatt tccgctgaat
781 atcgtggagc tcaccgccag aatcggtggt tgtggtgatg tacgtggcga actccgttgt
841 agtgcttgtg gtggcatccg tggcgcggcc gcggtaccag atctttaaat ctagataaag
901 aagtgacgcg gtctcaagcg tcgagcgtcg tcagcgtgtc gaggatgtcg aagtcgtagc
961 cgtcggcgct ggcgatgtag acctgctggt cgaattgact gtcgcgcata cacatcgggc
1021 cccggggccc gtcgaacccg acatcgtgcg cggatgccat caggtccggt atctcggggg
1081 agtgggcccg ctggaagatg gcctcgagcg caacgagacc ctcgtaacag gattcggcca
1141 tcgcgttgag cggtggcgcg tcggcgccgt agcgggcgac gtagctgccc atcaggtcca
1201 tggcacccgc ggtggccagt gaactgaagt acgccgcggc gacatagagg ttttcggtgg
1261 agccggcgcc gctggccagc agcatgttct cctccatcag cgggctgaac cgcgccatgc
1321 ggtcgtgccc gccggcgcgc gcgaactcgc ggttgaacaa cacggcgtcc tggccgacga
1381 gcagcatcaa cacggcctgc gcccccgacg cgatggcctt gcggaccggt gcgcggaaat
1441 cgtcggtgcc gtacgggacg tagatctccc gtctgagctc gaggtccaga tctcggcagt
1501 acgcgcgggc ggccgcggcg gaacggcgcg gccagatgta gtcatcgccg accaggcacc
1561 aggaccggat gccgaagtgg tcgcgcagcc aggcgagcgc gggcgcgatc tggatctgcg
1621 gtgtctcgcc tgtgcagaac acgcccggtg tgcgttcacc gccctcgtac aacgaggtgt
1681 agacgtacgg gatgcggtcg cggaccaccg gggagatgcg gttgcgcacg gccgagatgt
1741 gccagccggt cacggcgtcg agaccgtgac ctcgcaaccg gtcggcgacg gtccgggcga
1801 cgtcgtcgcc gggccgtccg ccgtcgagca cctcgatggt gaccttgcgg ccctgcaggc
1861 cgcctcggtc gttgacctcc ttggccgcga gctcggccac ggcctcgcac gaaggcgcga
1921 agattcccgc tggcccttga agcggaatca ccagcccgac gcggaactca acctcgccgt
1981 cctgcactcc agatcaccgt cgatcccgtg tagtctgcgc ttcaaagctt tctagcagaa
2041 ataattcatt ctgaacagac cccgccgtcg acacgaggag acacccacca tggccgccgg
2101 acagcagcgc cgccccaacc tcctgctgcc gttggtgcgt ctgacccacc tcgcggagtc
2161 ggcgatcgaa cgcgtgctcg cggactcgtc gctcaagatc gaggactggc gcgtgctcga
2221 cgagttggcc ggacggcgca ccgtgcccat gagcgatctc gcgcaggcca cgctgatcac
2281 gggtccgact ctcaccagaa ccgtcgatcg ccttgtgtcg caagggatca tctaccggac
2341 tgccgatctg catgaccgcc ggcgggtgct cgtggcgttg accccgcggg ggcggacgct
2401 gcgcaaccgc ctggtggacg cggtagccga ggccgagtgt gcggcttttg aatcgtgcgg
2461 gctggacgtc gaccagttgc gcgaactcgt cgacaccacc tcgaatttga cttcgtaacc
2521 acccgcgccc ggccggcgtt cacccttgac ttttattttc atctggatat atttcgggtg
2581 aatggaaagg ggtgaccatg ccgacctaca cattccgttg ttcccactgc ggtcccttcg
2641 atctcacctg cgcgatctcc gagcgcgatg cggcggcgac ctgtccggag tgccggacgc
2701 cggcgcgccg ggtcttcggt tcggtagggc tgacgacatt caccgcggga catcaccgcg
2761 cattcgacgc ggcgtccgcg agcgccgaaa gtcccacggt ggtgaagtcg attcccgcag
2821 gcgcggaccg cccgcgggcc ccgcgccgca atcccggtct accgagtctg ccgaggtact
2881 agcgacatgg gtggcgtcgg gctcttctac gtgggtgcgg tgctcatcat cgacgggctg
2941 atgctgctgg gccgcatcag cccacgaggc gcaacaccgc tgaacttctt cgtcggcgga
3001 ctgcaggtgg tgacgcctac ggtgctgatc ctgcagtccg gcggagacgc ggccgtgatc
3061 ttcgcggcct ccgggctcta cctgttcggc ttcacctacc tgtgggtggc catcaacaac
3121 gtgaccgact gggacggaga aggtctcgga tggttctcgc tgttcgtcgc gatcgccgca
3181 ctcggctact cgtggcacgc gttcaccgcc gaggccgacc cggcgttcgg ggtgatctgg
3241 ctgctgtggg cagtgctgtg gttcatgctg ttcctgctgc tcggcctggg gcacgacgca
3301 ctggggcccg ccgtcgggtt cgtcgcggtg gccgaaggcg tgatcaccgc cgccgtgccg
3361 gccttcctga tcgtgtcggg caactgggaa accggcccgc tccccgccgc ggtcatcgcc
3421 gtgatcggtt ttgccgcagt tgttctcgca taccccatcg ggcgccgtct cgcagcgccg
3481 tcagtcacca accctccacc ggccgcgctc gcggccacca cccgataaga gaaagggagt
3541 ccacatatgt aacggatcca gctgcagaat tcgaagctta tcgatgtcga cgtagttacg
3601 agatcggcgg ccgcatatga gtgtgcccac acaggacgga atgcaccggt tcgtcgacga
3661 ggacgtctac cacgctgacc ggggctcgct gtcggtatcc ggcgcgaagc tgctgttgcc
3721 gccgtcgtgt cccgcgaaat tccgctggga gatggacaac acccggaagc cgaaaaaggt
3781 ctgggacttc ggacatgtcg cgcacaaact ggtgctcggc aagggtgccg agttcgagat
3841 cctcgacccc gaggtgcacg ggctgaaggc ggacggtacg ccgtcggaga agccgaccgc
3901 gacgggcatg tggcgcaagg ccgaggctga ggctcgcaaa cagggcaagg tgccgattca
3961 cgtcgacctg ttcacgaagg cgtacgacat ggccgaaaag gtgcgtcagc acccgacagc
4021 cggcccgatc ttcgccaatc ctgacggcga ggccgaggtc gcgctgtact acaccgaccc
4081 cgagaccggc gtgcggctgc gtggccggat cgactggctc actgacgata tcgatgatta
4141 caagacgtcg atgaccgcga acccggccga gctgaaaacc aagttctaca agctcggcta
4201 tttcatgcag gcggcctggt acatcgatct actggtcgcc ctcgggctcg ccgagaaccc
4261 gcgattccgg ttcatcacgc aggagaaaga accgccctac gtcgtgactc cgatccagta
4321 cgacgacgag gcgatcgaag aggggcggcg ccgcaaccgc caggcgatcc ggctctacgc
4381 cgactgcatg gaatcgggca agtggcctga ctacagcgac gacgtggtca cgatcagcct
4441 gccctcgtgg gggctgccgc gaccgcagac cgtcggcgac gtcgtcaccg acagctatat
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