pJYS3_ΔcrtYf

$160.00

  • Model: PVT10658
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pJYS3_ΔcrtYf

PVT10658  2ug

 

pJYS3_ΔcrtYf Information

Prokaryotic resistance: kanamycin Kan

Cloning strain: Escherichia coli Stbl3

Culture conditions: 37 C

 

pJYS3_ΔcrtYf  Reference

CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum.

 

 

pJYS3_ΔcrtYf  Sequence

LOCUS       Exported               11982 bp ds-DNA     circular SYN 21-JAN-2018
FEATURES             Location/Qualifiers
     source          1..11982
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      2672..2894
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             2942..3892
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      6149..6234
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6326..6353
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(6397..10299)
                     /codon_start=1
                     /label=Cpf1
                     /translation="MSIYQEFVNKYSLSKTLRFELIPQGKTLENIKARGLILDDEKRAK
                     DYKKAKQIIDKYHQFFIEEILSSVCISEDLLQNYSDVYFKLKKSDDDNLQKDFKSAKDT
                     IKKQISEYIKDSEKFKNLFNQNLIDAKKGQESDLILWLKQSKDNGIELFKANSDITDID
                     EALEIIKSFKGWTTYFKGFHENRKNVYSSNDIPTSIIYRIVDDNLPKFLENKAKYESLK
                     DKAPEAINYEQIKKDLAEELTFDIDYKTSEVNQRVFSLDEVFEIANFNNYLNQSGITKF
                     NTIIGGKFVNGENTKRKGINEYINLYSQQINDKTLKKYKMSVLFKQILSDTESKSFVID
                     KLEDDSDVVTTMQSFYEQIAAFKTVEEKSIKETLSLLFDDLKAQKLDLSKIYFKNDKSL
                     TDLSQQVFDDYSVIGTAVLEYITQQIAPKNLDNPSKKEQELIAKKTEKAKYLSLETIKL
                     ALEEFNKHRDIDKQCRFEEILANFAAIPMIFDEIAQNKDNLAQISIKYQNQGKKDLLQA
                     SAEDDVKAIKDLLDQTNNLLHKLKIFHISQSEDKANILDKDEHFYLVFEECYFELANIV
                     PLYNKIRNYITQKPYSDEKFKLNFENSTLANGWDKNKEPDNTAILFIKDDKYYLGVMNK
                     KNNKIFDDKAIKENKGEGYKKIVYKLLPGANKMLPKVFFSAKSIKFYNPSEDILRIRNH
                     STHTKNGSPQKGYEKFEFNIEDCRKFIDFYKQSISKHPEWKDFGFRFSDTQRYNSIDEF
                     YREVENQGYKLTFENISESYIDSVVNQGKLYLFQIYNKDFSAYSKGRPNLHTLYWKALF
                     DERNLQDVVYKLNGEAELFYRKQSIPKKITHPAKEAIANKNKDNPKKESVFEYDLIKDK
                     RFTEDKFFFHCPITINFKSSGANKFNDEINLLLKEKANDVHILSIDRGERHLAYYTLVD
                     GKGNIIKQDTFNIIGNDRMKTNYHDKLAAIEKDRDSARKDWKKINNIKEMKEGYLSQVV
                     HEIAKLVIEYNAIVVFEDLNFGFKRGRFKVEKQVYQKLEKMLIEKLNYLVFKDNEFDKT
                     GGVLRAYQLTAPFETFKKMGKQTGIIYYVPAGFTSKICPVTGFVNQLYPKYESVSKSQE
                     FFSKFDKICYNLDKGYFEFSFDYKNFGDKAAKGKWTIASFGSRLINFRNSDKNHNWDTR
                     EVYPTKELEKLLKDYSIEYGHGECIKAAICGESDKKFFAKLTSVLNTILQMRNSKTGTE
                     LDYLISPVADVNGNFFDSRQAPKNMPQDADANGAYHIGLKGLMLLGRIKNNQEGKKLNL
                     VIKNEEYFEFVQNRNN"
     CDS             complement(10392..11186)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=Kan
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 tcctcacgta gtgccaaagg aacgcgtgcg gtgaccacga cgggcttagc ctttgcctgc
       61 gcttctagtg cttcgatggt ggcttgtgcc tgcgcttgct gcgcctgtag tgcctgttga
      121 gcttcttgta gttgctgttc tagctgtgcc ttggttgcca tgctttaaga ctctagtagc
      181 tttcctgcga tatgtcatgc gcatgcgtag caaacattgt cctgcaactc attcattatg
      241 tgcagtgctc ctgttactag tcgtacatac tcatatttac ctagtctgca tgcagtgcat
      301 gcacatgcag tcatgtcgtg ctaatgtgta aaacatgtac atgcagattg ctgggggtgc
      361 agggggcgga gccaccctgt ccatgcgggg tgtggggctt gccccgccgg tacagacagt
      421 gagcaccggg gcacctagtc gcggataccc cccctaggta tcggacacgt aaccctccca
      481 tgtcgatgca aatctttaac attgagtacg ggtaagctgg cacgcatagc caagctaggc
      541 ggccaccaaa caccactaaa aattaatagt ccctagacaa gacaaacccc cgtgcgagct
      601 accaactcat atgcacgggg gccacataac ccgaaggggt ttcaattgac aaccatagca
      661 ctagctaaga caacgggcac aacacccgca caaactcgca ctgcgcaacc ccgcacaaca
      721 tcgggtctag gtaacactga aatagaagtg aacacctcta aggaaccgca ggtcaatgag
      781 ggttctaagg tcactcgcgc tagggcgtgg cgtaggcaaa acgtcatgta caagatcacc
      841 aatagtaagg ctctggcggg gtgccatagg tggcgcaggg acgaagctgt tgcggtgtcc
      901 tggtcgtcta acggtgcttc gcagtttgag ggtctgcaaa actctcactc tcgctggggg
      961 tcatctctgg ctgaattgga agtcatgggc gaacgccgca ttgagctggc tattgctact
     1021 aagaatcact tggcggcggg tggcgcgctc atgatgtttg tgggcactgt tcgacacaac
     1081 cgctcacagt catttgcgca ggttgaagcg ggtattaaga ctgcgtactc ttcgatggtg
     1141 aaaacatctc agtggaagaa agaacgtgca cggtacgggg tggagcacac ctatagtgac
     1201 tatgaggtca cagactcttg ggcgaacggt tggcacttgc accgcaacat gctgttgttc
     1261 ttggatcgtc cactgtctga cgatgaactc aaggcgtttg aggattccat gttttcccgc
     1321 tggtctgctg gtgtggttaa ggccggtatg gacgcgccac tgcgtgagca cggggtcaaa
     1381 cttgatcagg tgtctacctg gggtggagac gctgcgaaaa tggcaaccta cctcgctaag
     1441 ggcatgtctc aggaactgac tggctccgct actaaaaccg cgtctaaggg gtcgtacacg
     1501 ccgtttcaga tgttggatat gttggccgat caaagcgacg ccggcgagga tatggacgct
     1561 gttttggtgg ctcggtggcg tgagtatgag gttggttcta aaaacctgcg ttcgtcctgg
     1621 tcacgtgggg ctaagcgtgc tttgggcatt gattacatag acgctgatgt acgtcgtgaa
     1681 atggaagaag aactgtacaa gctcgccggt ctggaagcac cggaacgggt cgaatcaacc
     1741 cgcgttgctg ttgctttggt gaagcccgat gattggaaac tgattcagtc tgatttcgcg
     1801 gttaggcagt acgttctcga ttgcgtggat aaggctaagg acgtggccgc tgcgcaacgt
     1861 gtcgctaatg aggtgctggc aagtctgggt gtggattcca ccccgtgcat gatcgttatg
     1921 gatgatgtgg acttggacgc ggttctgcct actcatgggg acgctactaa gcgtgatctg
     1981 aatgcggcgg tgttcgcggg taatgagcag actattcttc gcacccacta aaagcggcat
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