pKC1139 Plasmid

$216.00

  • Model: PVT6009
  • 50 Units in Stock
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pKC1139

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pKC1139,Plasmid pKC1139,pKC1139 vector

pKC1139 Plasmid information

Plasmid size: 6573bp
Prokaryotic Apramycin resistance: Apr
Clone strain: DH5 alpha and other Escherichia coli
Culture conditions: 30 DEG C, aerobic, LB
Expressing host: Streptomyces, actinomyces
5'sequencing: M13 (-21) Forward:TGTAAAACGACGGCCAGT
3'sequencing: M13-R: CAGGAAACAGCTATGAC
Use:Homologous recombination HR plasmid

pKC1139 Plasmid Sequence
LOCUS       pKC1139         6573 bp    DNA     circular     14-MAR-2012
FEATURES             Location/Qualifiers
     CDS             complement(167..508)
                     /vntifkey="4"
                     /label=lacZa
     CDS             1730..2503
                     /vntifkey="4"
                     /label=aac(3)IV
     primer          complement(509..525)
                     /vntifkey="27"
                     /label=sp6
     primer          complement(528..547)
                     /vntifkey="27"
                     /label=seq-152-anti
     misc_feature    765..934
                     /vntifkey="21"
                     /label=ori-PUC18
     misc_feature    6050..6161
                     /vntifkey="21"
                     /label=oriT
     CDS             complement(3443..4885)
                     /vntifkey="4"
                     /label=Rep-pSG5
     promoter        complement(4855..5273)
                     /vntifkey="30"
                     /label=promoter
     promoter        1662..1711
                     /vntifkey="30"
                     /label=promoter
BASE COUNT     1180 a      2002 c      2082 g      1309 t 
ORIGIN
        1 gatctacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc 
       61 agctctcgca gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg 
      121 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt gatcggcact 
      181 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattt atgcggtgtg 
      241 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc 
      301 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga 
      361 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac 
      421 gttgtaaaac gacggccagt gccaagcttg ggctgcaggt cgactctaga ggatccgcgg 
      481 ccgcgcgcga tatcgaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc 
      541 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 
      601 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 
      661 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 
      721 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 
      781 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 
      841 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 
      901 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 
      961 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 
     1021 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 
     1081 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 
     1141 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 
     1201 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 
     1261 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 
     1321 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 
     1381 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 
     1441 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 
     1501 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 
     1561 ggattttggt catgagatta tcaaaaagga tcttcaccta gatccttttg gttcatgtgc 
     1621 agctccatca gcaaaagggg atgataagtt tatcaccacc gactatttgc aacagtgccg 
     1681 ttgatcgtgc tatgatcgac tgatgtcatc agcggtggag tgcaatgtcg tgcaatacga 
     1741 atggcgaaaa gccgagctca tcggtcagct tctcaacctt ggggttaccc ccggcggtgt 
     1801 gctgctggtc cacagctcct tccgtagcgt ccggcccctc gaagatgggc cacttggact 
     1861 gatcgaggcc ctgcgtgctg cgctgggtcc gggagggacg ctcgtcatgc cctcgtggtc 
     1921 aggtctggac gacgagccgt tcgatcctgc cacgtcgccc gttacaccgg accttggagt 
     1981 tgtctctgac acattctggc gcctgccaaa tgtaaagcgc agcgcccatc catttgcctt 
     2041 tgcggcagcg gggccacagg cagagcagat catctctgat ccattgcccc tgccacctca 
     2101 ctcgcctgca agcccggtcg cccgtgtcca tgaactcgat gggcaggtac ttctcctcgg 
     2161 cgtgggacac gatgccaaca cgacgctgca tcttgccgag ttgatggcaa aggttcccta 
     2221 tggggtgccg agacactgca ccattcttca ggatggcaag ttggtacgcg tcgattatct 
     2281 cgagaatgac cactgctgtg agcgctttgc cttggcggac aggtggctca aggagaagag 
     2341 ccttcagaag gaaggtccag tcggtcatgc ctttgctcgg ttgatccgct cccgcgacat 
     2401 tgtggcgaca gccctgggtc aactgggccg agatccgttg atcttcctgc atccgccaga 
     2461 ggcgggatgc gaagaatgcg atgccgctcg ccagtcgatt ggctgagctc atgagcggag 
     2521 aacgagatga cgttggaggg gcaaggtcgc gctgattgct ggggcaacac gtggagcgga 
     2581 tcggggattg tctttcttca gctcgctgat gatatgctga cgctcaatgc cgtttggcct 
     2641 ccgactaacg aaaatcccgc atttggacgg ctgatccgat tggcacggcg gacggcgaat 
     2701 ggcggagcag acgctcgtcc gggggcaatg agatatgaaa aagcctgaac tcaccgcgac 
     2761 gtatcgatgt cgaggttcct caggggagcc accccagaga agccctcgga gctgagcgga 
     2821 gctatttcca aagccatgcc agctagagac agtgcacacc gccaagcatc tgcaaaaccc 
     2881 tcgcctggag ggaaagtgca atgtacgtac gctggtttcc ctccagaaag ggattctgcg 
     2941 gcttctcaac tttggaagaa gaggcggaac gaactgctgt ttcagcctac tcatgtgagg 
     3001 aggctggtcc tttaccctga tgcccgaggg catcagggta aaggaccagc ctcgccaact 
     3061 aggagcgcta ccaaggcgaa cacaacccag gacttggtag aacccttcgc cggagaagtt 
     3121 cagactcatc ggcatgagca cccctcgccg cgcactcggc accggtccgt cgaccaccac 
     3181 caggacgtcg ttgtcgacgt cggccccgcg gctcctgccc gccgaacgcg tcgtcgtcga 
     3241 cggcctggtg ctcatcgacg agcacccgga gccaggtgaa aagcgccggc ggacgctcgg 
     3301 actgggcgcg ggattccagc agtaacccag gtccgccggc cacctcacgg caggcagacc 
     3361 ctcggctttc gcgccgggac cgccatgaga ccgccacccg gatgtccggg gtggcggtct 
     3421 catgggcggt ccctcagcgg ccctacgcga ccgctgtgtc gacgcggagg cagtctccgg 
     3481 ggtgctggcc ccagggctgg agtaccgggg cgagcttgcc cttgcagcgg cggcacacgg 
     3541 gcgacgcggc gccgggcggg ggagtcttga cgtcgacggt gaccggcttc ggcttcaggc 
     3601 gcttccgaag gctgatcgtc gggcggatcg cctccttctt cggctgacgc actcggtcga 
     3661 gcgaagcggc cagctcctcc gtgcgggcct cgttggcctt ccggcgcgcc tcgcggaaag 
     3721 cagcttcctc ctcggacatg acctcgaacc tcatctggtc agcgtcaagg tcgcccggcg 
     3781 cggccggcgc ttccggggcg ggcgggtcca ggacgtcctt gccccacacc aggccccagg 
     3841 actcgacgag ccgccggacg cccggtaggc cgtacgtctc ggcgaccttg atgaggtcga 
     3901 ggcgacgtcc ggcgacgcgg gcgatgtatc ggtaccagat gtaggccggg atgaccgcga 
     3961 tggcgaccag gccctcggtg tcgtcggtga tctcctcctc ggtgcggacg tcctgctgga 
     4021 tgccgagttc cttgatcagc cggttcaggt tctgcgaccg gtagtgcttg cggacctgga 
     4081 agacgccgaa ctcgcgctcg cggtacttct cgacgaacgg gccgggccga cgaagccgct 
     4141 gcagctcggc ggccgccgcg tcgcccaggt cgagcggtcc catgcggtcg tcgccgcgac 
     4201 cggccttgaa gttctgtccg gccagctcca ggccgatctt ggcgacgccg cccttggtct 
     4261 tgtcgccgtc cttgtagagg tagcgggcct gcttgcccgc atcgccgtca gcggcgtccg 
     4321 cgccgttgag tgggcgcacg tcggtgccgt ggcccttgcc ctcacaggag caaccgggcc 
     4381 ggtcgcacgt ctcgctgacg gtgtagccgc ccgcggattc gaccccggcg gcccaggctc 
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