pKD13

$216.00

  • Model: PVT6003
  • 50 Units in Stock
Ask a question


Add to Cart:

pKD13

PVT6003    2ug

 

pKD13 Plasmid Information

Carrier name: pKD13

Plasmid type: bacterial expression; gene knockout system

High copy / low copy: -

Promoter: -

Cloning methods: polyclonal sites, restrictive endonucleases

Carrier size: 3434 BP

Use:Homologous recombination HR plasmid

 

pKD13 Plasmid Sequence

  LOCUS       pKD13                3434 bp    DNA     circular SYN 11-SEP-2001  DEFINITION  Template plasmid pKD13, complete sequence.  ACCESSION   AY048744  VERSION     AY048744.1  GI:15554335  KEYWORDS    .  SOURCE      Template plasmid pKD13    ORGANISM  Template plasmid pKD13              other sequences; artificial sequences; vectors.  REFERENCE   1  (bases 1 to 3434)    AUTHORS   Datsenko,K.A. and Wanner,B.L.    TITLE     One-step inactivation of chromosomal genes in Escherichia coli K-12              using PCR products    JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (12), 6640-6645 (2000)     PUBMED   10829079  REFERENCE   2  (bases 1 to 3434)    AUTHORS   Datsenko,K.A. and Wanner,B.L.    TITLE     Direct Submission    JOURNAL   Submitted (29-JUL-2001) Biological Sciences, Purdue University,              Lilly Hall of Life Sciences, West Lafayette, IN 47907, USA  FEATURES             Location/Qualifiers       source          1..3434                       /organism="Template plasmid pKD13"                       /mol_type="genomic DNA"                       /db_xref="taxon:170493"       misc_feature    31..50                       /note="priming site 1"       misc_feature    complement(51..98)                       /note="natural FRT site"       CDS             complement(111..905)                       /note="kanamycin resistance"                       /codon_start=1                       /transl_table=11                       /product="Tn5 neomycin phosphotransferase"                       /protein_id="AAL02037.1"                       /db_xref="GI:15554336"       misc_feature    274..293                       /note="common priming site kt"       misc_feature    744..763                       /note="common priming site k2"       misc_feature    835..854                       /note="common priming site k1"       misc_feature    complement(1273..1307)                       /note="distal 35-nt of natural FRT site"       misc_feature    1334..1353                       /note="priming site 4"       terminator      1402..1644                       /note="lambda terminator"       rep_origin      1678..2076                       /note="R6K gamma replication origin"       CDS             complement(2146..3006)                       /note="bla; ampicillin resistance"                       /codon_start=1                       /transl_table=11                       /product="beta-lactamase"                       /protein_id="AAL02038.1"                       /db_xref="GI:15554337"       terminator      3153..3412                       /note="rrnB transcription terminator"  ORIGIN                1 agattgcagc attacacgtc ttgagcgatt gtgtaggctg gagctgcttc gaagttccta         61 tactttctag agaataggaa cttcggaata ggaacttcaa gatcccctta ttagaagaac        121 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc        181 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac        241 gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag        301 cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc        361 tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga        421 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc        481 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc        541 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg        601 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg        661 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg        721 tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc        781 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca        841 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca        901 atcatgcgaa acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag        961 atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag       1021 ggcgccccag ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat       1081 cgccatgtaa gcccactgca agctacctgc tttctctttg cgcttgcgtt ttcccttgtc       1141 cagatagccc agtagctgac attcatccgg ggtcagcacc gtttctgcgg actggctttc       1201 tacgtgttcc gcttccttta gcagcccttg cgccctgagt gcttgcggca gcgtgagctt       1261 caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcgaac tgcaggtcga       1321 cggatccccg gaattaattc tcatgtttga cagcttatca ctgatcagtg aattaatggc       1381 gatgacgcat cctcacgata atatccgggt aggcgcaatc actttcgtct ctactccgtt       1441 acaaagcgag gctgggtatt tcccggcctt tctgttatcc gaaatccact gaaagcacag       1501 cggctggctg aggagataaa taataaacga ggggctgtat gcacaaagca tcttctgttg       1561 agttaagaac gagtatcgag atggcacata gccttgctca aattggaatc aggtttgtgc       1621 caataccagt agaaacagac gaagaagcta gctttgcact ggattgcgag gctttgccat       1681 ggctaattcc catgtcagcc gttaagtgtt cctgtgtcac tgaaaattgc tttgagaggc       1741 tctaagggct tctcagtgcg ttacatccct ggcttgttgt ccacaaccgt taaaccttaa       1801 aagctttaaa agccttatat attctttttt ttcttataaa acttaaaacc ttagaggcta       1861 tttaagttgc tgatttatat taattttatt gttcaaacat gagagcttag tacgtgaaac       1921 atgagagctt agtacgttag ccatgagagc ttagtacgtt agccatgagg gtttagttcg       1981 ttaaacatga gagcttagta cgttaaacat gagagcttag tacgtgaaac atgagagctt       2041 agtacgtact atcaacaggt tgaactgcgg atcttgcggc cgcaaaaatt aaaaatgaag       2101 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat       2161 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc       2221 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat       2281 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag       2341 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg       2401 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc       2461 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca       2521 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg       2581 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc       2641 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta       2701 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc       2761 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg       2821 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc       2881 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc       2941 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat       3001 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag       3061 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc       3121 ccgaaaagtg ccacctgcat cgatggcccc ccgatggtag tgtggggtct ccccatgcga       3181 gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt       3241 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg       3301 gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact       3361 gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtggccagtg       3421 ccaagcttgc atgc  ///

 

Caution:
Product is for research use only!

 

Search name

pKD13,Plasmid pKD13,pKD13 vector

No customer comments for the moment.

Add A Comment

Related Products

Cart  

No products

Total $0.00

Prices don't include postage.

Cart