pKD20 Plasmid

$396.00

  • Model: PVT6004
  • 50 Units in Stock
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pKD20

Search name

pKD20,Plasmid pKD20,pKD20 vector

Bacterial Resistance:Ampicillin
vector size 6078 bp
Use:Homologous recombination HR plasmid

pKD20 Sequence

  LOCUS       AY048745                6078 bp    DNA     circular SYN 11-SEP-2001  DEFINITION  Red recombinase plasmid pKD20, complete sequence.  ACCESSION   AY048745  VERSION     AY048745.1  GI:15554338  KEYWORDS    .  SOURCE      Red recombinase plasmid pKD20    ORGANISM  Red recombinase plasmid pKD20              other sequences; artificial sequences; vectors.  REFERENCE   1  (bases 1 to 6078)    AUTHORS   Datsenko,K.A. and Wanner,B.L.    TITLE     One-step inactivation of chromosomal genes in Escherichia coli K-12              using PCR products    JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (12), 6640-6645 (2000)     PUBMED   10829079  REFERENCE   2  (bases 1 to 6078)    AUTHORS   Datsenko,K.A. and Wanner,B.L.    TITLE     Direct Submission    JOURNAL   Submitted (29-JUL-2001) Biological Sciences, Purdue University,              Lilly Hall of Life Sciences, West Lafayette, IN 47907, USA  FEATURES             Location/Qualifiers       source          1..6078                       /organism="Red recombinase plasmid pKD20"                       /mol_type="genomic DNA"                       /db_xref="taxon:170494"       CDS             complement(11..889)                       /codon_start=1                       /transl_table=11                       /product="araC protein"                       /protein_id="AAL02039.1"                       /db_xref="GI:15554339"         misc_feature    complement(1040..1068)                       /note="Pc"       misc_feature    1165..1192                       /note="ParaB"       CDS             1244..1660                       /note="recombination;138"                       /codon_start=1                       /transl_table=11                       /product="gam protein"                       /protein_id="AAL02040.1"                       /db_xref="GI:15554340"         CDS             1666..2451                       /note="recombination;261"                       /codon_start=1                       /transl_table=11                       /product="bet protein"                       /protein_id="AAL02041.1"                       /db_xref="GI:15554341"         CDS             2448..3128                       /note="exo"                       /codon_start=1                       /transl_table=11                       /product="exonuclease"                       /protein_id="AAL02042.1"                       /db_xref="GI:15554342"         CDS             complement(3245..4195)                       /codon_start=1                       /transl_table=11                       /product="repA101 protein"                       /protein_id="AAL02043.1"                       /db_xref="GI:15554343"         rep_origin      4199..4939                       /note="oriR101"       CDS             complement(5087..5947)                       /note="bla; ampicillin resistance"                       /codon_start=1                       /transl_table=11                       /product="beta-lactamase"                       /protein_id="AAL02044.1"                       /db_xref="GI:15554344"    ORIGIN                1 catcgattta ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac         61 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat        121 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca        181 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct        241 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga        301 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat        361 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct        421 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga        481 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg        541 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt        601 aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc        661 ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca        721 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt        781 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg        841 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac        901 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg        961 tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt       1021 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca       1081 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat       1141 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat       1201 acccgttttt ttgggaattc gagctctaag gaggttataa aaaatggata ttaatactga       1261 aactgagatc aagcaaaagc attcactaac cccctttcct gttttcctaa tcagcccggc       1321 atttcgcggg cgatattttc acagctattt caggagttca gccatgaacg cttattacat       1381 tcaggatcgt cttgaggctc agagctgggc gcgtcactac cagcagctcg cccgtgaaga       1441 gaaagaggca gaactggcag acgacatgga aaaaggcctg ccccagcacc tgtttgaatc       1501 gctatgcatc gatcatttgc aacgccacgg ggccagcaaa aaatccatta cccgtgcgtt       1561 tgatgacgat gttgagtttc aggagcgcat ggcagaacac atccggtaca tggttgaaac       1621 cattgctcac caccaggttg atattgattc agaggtataa aacgaatgag tactgcactc       1681 gcaacgctgg ctgggaagct ggctgaacgt gtcggcatgg attctgtcga cccacaggaa       1741 ctgatcacca ctcttcgcca gacggcattt aaaggtgatg ccagcgatgc gcagttcatc       1801 gcattactga tcgttgccaa ccagtacggc cttaatccgt ggacgaaaga aatttacgcc       1861 tttcctgata agcagaatgg catcgttccg gtggtgggcg ttgatggctg gtcccgcatc       1921 atcaatgaaa accagcagtt tgatggcatg gactttgagc aggacaatga atcctgtaca       1981 tgccggattt accgcaagga ccgtaatcat ccgatctgcg ttaccgaatg gatggatgaa       2041 tgccgccgcg aaccattcaa aactcgcgaa ggcagagaaa tcacggggcc gtggcagtcg       2101 catcccaaac ggatgttacg tcataaagcc atgattcagt gtgcccgtct ggccttcgga       2161 tttgctggta tctatgacaa ggatgaagcc gagcgcattg tcgaaaatac tgcatacact       2221 gcagaacgtc agccggaacg cgacatcact ccggttaacg atgaaaccat gcaggagatt       2281 aacactctgc tgatcgccct ggataaaaca tgggatgacg acttattgcc gctctgttcc       2341 cagatatttc gccgcgacat tcgtgcatcg tcagaactga cacaggccga agcagtaaaa       2401 gctcttggat tcctgaaaca gaaagccgca gagcagaagg tggcagcatg acaccggaca       2461 ttatcctgca gcgtaccggg atcgatgtga gagctgtcga acagggggat gatgcgtggc       2521 acaaattacg gctcggcgtc atcaccgctt cagaagttca caacgtgata gcaaaacccc       2581 gctccggaaa gaagtggcct gacatgaaaa tgtcctactt ccacaccctg cttgctgagg       2641 tttgcaccgg tgtggctccg gaagttaacg ctaaagcact ggcctgggga aaacagtacg       2701 agaacgacgc cagaaccctg tttgaattca cttccggcgt gaatgttact gaatccccga       2761 tcatctatcg cgacgaaagt atgcgtaccg cctgctctcc cgatggttta tgcagtgacg       2821 gcaacggcct tgaactgaaa tgcccgttta cctcccggga tttcatgaag ttccggctcg       2881 gtggtttcga ggccataaag tcagcttaca tggcccaggt gcagtacagc atgtgggtga       2941 cgcgaaaaaa tgcctggtac tttgccaact atgacccgcg tatgaagcgt gaaggcctgc       3001 attatgtcgt gattgagcgg gatgaaaagt acatggcgag ttttgacgag atcgtgccgg       3061 agttcatcga aaaaatggac gaggcactgg ctgaaattgg ttttgtattt ggggagcaat       3121 ggcgatgata agctagccca tgggtatgga cagttttccc tttgatatgt aacggtgaac       3181 agttgttcta cttttgtttg ttagtcttga tgcttcactg atagatacaa gagccataag       3241 aacctcagat ccttccgtat ttagccagta tgttctctag tgtggttcgt tgtttttgcg       3301 tgagccatga gaacgaacca ttgagatcat acttactttg catgtcactc aaaaattttg       3361 cctcaaaact ggtgagctga atttttgcag ttaaagcatc gtgtagtgtt tttcttagtc       3421 cgttacgtag gtaggaatct gatgtaatgg ttgttggtat tttgtcacca ttcattttta       3481 tctggttgtt ctcaagttcg gttacgagat ccatttgtct atctagttca acttggaaaa       3541 tcaacgtatc agtcgggcgg cctcgcttat caaccaccaa tttcatattg ctgtaagtgt       3601 ttaaatcttt acttattggt ttcaaaaccc attggttaag ccttttaaac tcatggtagt       3661 tattttcaag cattaacatg aacttaaatt catcaaggct aatctctata tttgccttgt       3721 gagttttctt ttgtgttagt tcttttaata accactcata aatcctcata gagtatttgt       3781 tttcaaaaga cttaacatgt tccagattat attttatgaa tttttttaac tggaaaagat       3841 aaggcaatat ctcttcacta aaaactaatt ctaatttttc gcttgagaac ttggcatagt       3901 ttgtccactg gaaaatctca aagcctttaa ccaaaggatt cctgatttcc acagttctcg       3961 tcatcagctc tctggttgct ttagctaata caccataagc attttcccta ctgatgttca       4021 tcatctgagc gtattggtta taagtgaacg ataccgtccg ttctttcctt gtagggtttt       4081 caatcgtggg gttgagtagt gccacacagc ataaaattag cttggtttca tgctccgtta       4141 agtcatagcg actaatcgct agttcatttg ctttgaaaac aactaattca gacatacatc       4201 tcaattggtc taggtgattt taatcactat accaattgag atgggctagt caatgataat       4261 tactagtcct tttcctttga gttgtgggta tctgtaaatt ctgctagacc tttgctggaa       4321 aacttgtaaa ttctgctaga ccctctgtaa attccgctag acctttgtgt gttttttttg       4381 tttatattca agtggttata atttatagaa taaagaaaga ataaaaaaag ataaaaagaa       4441 tagatcccag ccctgtgtat aactcactac tttagtcagt tccgcagtat tacaaaagga       4501 tgtcgcaaac gctgtttgct cctctacaaa acagacctta aaaccctaaa ggcttaagta       4561 gcaccctcgc aagctcggtt gcggccgcaa tcgggcaaat cgctgaatat tccttttgtc       4621 tccgaccatc aggcacctga gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac       4681 ggctctggca gtgaatgggg gtaaatggca ctacaggcgc cttttatgga ttcatgcaag       4741 gaaactaccc ataatacaag aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg       4801 tctgctatgt ggtgctatct gactttttgc tgttcagcag ttcctgccct ctgattttcc       4861 agtctgacca cttcggatta tcccgtgaca ggtcattcag actggctaat gcacccagta       4921 aggcagcggt atcatcaacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga       4981 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa       5041 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa       5101 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc       5161 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga       5221 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa       5281 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt       5341 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg       5401 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc       5461 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg       5521 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag       5581 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt       5641 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt       5701 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac       5761 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac       5821 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag       5881 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa       5941 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga       6001 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc       6061 cccgaaaagt gccacctg  //
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