pKD46 Plasmid

$216.00

  • Model: PVT6005
  • 50 Units in Stock
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pKD46

PVT6005     100ul

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pKD46,Plasmid pKD46,pKD46 vector

 

pKD46 Information

Promoter: araBAD promoter

Replicon: pSC101 ori

Terminator: lambda tL3 Terminator

Prokaryotic resistance: ampicillin Amp

Cloned strain: Escherichia coli DH5 alpha

Culture conditions: 30 degrees centigrade

Expression host: Escherichia coli

Inducement: Arabia sugar

Use:Homologous recombination HR plasmid

 

pKD46 Description

PKD46 plasmid is the most widely used Red homologous recombination plasmid currently. The plasmid contains exo, bet and gam genes regulated by Arabia sugar promoter, and its replicon is low copy temperature sensitive replicon, which is convenient for plasmid elimination.

Related 3 Enzymesto the knockout of homologous recombination genes:

1.Exo protein

Exo protein is an exonuclease, single subunit molecular weight is 24 kD, the active form of Exo protein is a cyclic trimer, a hollow passage, one end of the channel can accommodate a double stranded DNA molecule, the other end can only contain a single stranded DNA. The Exo protein can be combined at the end of the double stranded DNA and degrade DNA from the 5 'end of the DNA double strand to the 3' end, causing the DNA molecule to form a 3 'sticky end.

2. Beta protein

Beta protein plays a decisive role in Red homologous recombination, is mediated by complementary single strand annealing of the DNA function, which is an annealing protein, the molecular weight of single subunit was 25.8 kD. In solution, Beta protein spontaneously forms ring structure, tightly bound to the 3 'prominent end of single stranded DNA, preventing DNA from being degraded by single chain nuclease and mediating the annealing of complementary single strand DNA. After the double stranded DNA was annealed, the Beta protein was dissociated from the double strand of DNA.

3. Gam protein

Gam protein is a polypeptide molecule of 16kD, can be combined with RecBCD exonuclease, inhibiting the degradation of exogenous DNA, to prevent foreign DNA into cells after degradation by the host.

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