pKK232-8
Search name
pKK232-8,Plasmid pKK232-8,pKK232-8 vector
pKK232-8 Information
Replicon: pBR322 ori
Terminator: rrnB T1
Plasmid classification: Escherichia coli vector; protein expression plasmid
Plasmid size: 5094bp
Prokaryotic resistance: ampicillin Amp
Cloned strain: DH5 alpha
Culture conditions: 37 centigrade, aerobic, LB
Expression host: Escherichia coli
Culture conditions: 37 centigrade, aerobic, LB
Remarks: expression plasmids
pKK232-8 Descrption
PKK232-8 is a pKK series expression plasmid of Escherichia coli.
pKK232-8 Sequence
LOCUS Exported 5094 bp ds-DNA circular SYN 18-SEP-2016
DEFINITION synthetic circular DNA
KEYWORDS Untitled 5
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5094)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported 2016-9-18 from SnapGene Viewer 2.8.1
FEATURES Location/Qualifiers
source 1..5094
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 12..98
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS 269..928
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
terminator 1363..1449
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1541..1568
/note="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 1861..1947
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 2039..2066
/note="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
CDS 2648..2839
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/note="rop"
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
misc_feature 2941..3081
/note="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(3267..3855)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4026..4886)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4887..4991)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 ttcccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
61 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg
121 ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccagggaat
181 tcccggggat ccgtcgacct gcagccaagc ttgagtagga caaatccgcc gagcttcgac
241 gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt
301 tgatatatcc caatcgcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg
361 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa
421 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc
481 ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg
541 ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga
601 cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct
661 ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt
721 gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt
781 caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt
841 tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta
901 ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt gcccttaaac
961 gcctggtgct acgcctgaat aagtgataat aagcggatga atggcagaaa ttcgtcgagg
1021 cggcacctcg ctaacggatt caccactcca agaattggag ccaatcaatt cttgcggaga
1081 actgtgaatg cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc catctccagc
1141 agccgcacgc ggcgcatctc ggctgttttg gcggatgaga gaagattttc agcctgatac
1201 agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg
1261 cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta
1321 gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct
1381 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
1441 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg
1501 gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat
1561 ggcctttttg cgtttctaca aactcttcct gtcgtcatat ctacaagcca tccccccaca
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