pKW08- Lx

$193.00

  • Model: PVT11355
  • 50 Units in Stock
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pKW08-Lx


Catalog No. PVT11355
Packing 2ug

 

pKW08-Lx Information

Replicon: ColE1 ori

Terminator: rrnB T2 Terminator

Plasmid classification: mycobacterial plasmid

Plasmid size: 7960bp

Plasmid tagging: LuxA, LuxB

Prokaryotic resistance: hygromycin Hygromycin

Clonal strain: DH5 alpha

Culture conditions: 37 C, aerobic, LB

Expression host: Mycobacterium

5'sequencing primers: Based on full sequence design

 

pKW08-Lx Sequence

LOCUS       Exported                7960 bp ds-DNA     circular SYN 30-AUG-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pKW08-Lx
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, August 30, 2016 from SnapGene Viewer 3.1.4
          
FEATURES             Location/Qualifiers
     source          1..7960
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(248..836)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5042..5058
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      7920..7947
                     /note="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 actagttcta gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
       61 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      121 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      181 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      241 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      301 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      361 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      421 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      481 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      541 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      601 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
      661 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
      721 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
      781 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
      841 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
      901 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
      961 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     1021 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     1081 ccggacgtgg ccgaccagcc cgtcatcgtc aacgcctgac cgcggtgcgg acaggccgtg
     1141 tcgcgaccgg ccgtgcggaa ttaagccggc ccgtaccctg tgaatagagg tccgctgtga
     1201 cacaagaatc cctgttactt ctcgaccgta ttgattcgga tgattcctac gcgagcctgc
     1261 ggaacgacca ggaattctgg gagccgctgg cccgccgagc cctggaggag ctcgggctgc
     1321 cggtgccgcc ggtgctgcgg gtgcccggcg agagcaccaa ccccgtactg gtcggcgagc
     1381 ccgacccggt gatcaagctg ttcggcgagc actggtgcgg tccggagagc ctcgcgtcgg
     1441 agtcggaggc gtacgcggtc ctggcggacg ccccggtgcc ggtgccccgc ctcctcggcc
     1501 gcggcgagct gcggcccggc accggagcct ggccgtggcc ctacctggtg atgagccgga
     1561 tgaccggcac cacctggcgg tccgcgatgg acggcacgac cgaccggaac gcgctgctcg
     1621 ccctggcccg cgaactcggc cgggtgctcg gccggctgca cagggtgccg ctgaccggga
     1681 acaccgtgct caccccccat tccgaggtct tcccggaact gctgcgggaa cgccgcgcgg
     1741 cgaccgtcga ggaccaccgc gggtggggct acctctcgcc ccggctgctg gaccgcctgg
     1801 aggactggct gccggacgtg gacacgctgc tggccggccg cgaaccccgg ttcgtccacg
     1861 gcgacctgca cgggaccaac atcttcgtgg acctggccgc gaccgaggtc accgggatcg
     1921 tcgacttcac cgacgtctat gcgggagact cccgctacag cctggtgcaa ctgcatctca
     1981 acgccttccg gggcgaccgc gagatcctgg ccgcgctgct cgacggggcg cagtggaagc
     2041 ggaccgagga cttcgcccgc gaactgctcg ccttcacctt cctgcacgac ttcgaggtgt
     2101 tcgaggagac cccgctggat ctctccggct tcaccgatcc ggaggaactg gcgcagttcc
     2161 tctgggggcc gccggacacc gcccccggcg cctgacgccc cgggccgccc ggcgccgccc
     2221 ccggcccccg gcggccgccc ggagccccgc ccgcgctcgg gagccccggg cccgcgccga
     2281 agcccgctgc tgcgagcccg gagcgggccg gccgacggcg gtgcgggccc ggcggcggac
     2341 gctcagcagc ggcgggcgtg aaaggccctg gcatcctcga tcatctcctc cagggtggtc
     2401 ggccgagctt ccatcccagc tcggcaagga tcaacgagga cagtcgcacg acgaagttct
     2461 tctggatcgc gcccgtgctg gaagcactca acctcgaagc gtgtggttgc ggagccatct
     2521 agcaaccaca cgaaacatgc gcaacgaacc gcgcaacgaa caacgcctag aactggccct
     2581 agatgagctg actcgtatcg ttggtaaacc tagtttgacc agcatgtttt aactacgttc
     2641 ggtgagctgt caacggggcc tgtaacggca caacgaaccg tgcaacgaga gtggccacgg
     2701 atgccaccac aggcactaca acggagttcg ccacgtacat caccacaacc accgattctg
     2761 gcggtgagct ccccgatatt cagcggaaat ggcttggtat cgaccaagat tcgtagaacc
     2821 ccgtctcgtc tggctggtat tcaaaacggg cgcaacgaaa cacgcaacga gacaggcatg
     2881 gcccaaacca gaaaactagc gtctaccagg acttttacct gtccgacccg ttgcaacgga
     2941 accccccacg gaacccccgc gacacccgct ccccaattgc gttagaacag cggtggattg
     3001 tcggcttcgt tgtgggcctt ttgagccgct tcctgttctg ccgcacgctc tttcctcgcc
     3061 cgatagccga gtcgcttaac ggtgtccaga tgcagcccga aatgtttggc cgtttgcggc
     3121 caagagtggc cctcgtcgtc gtgataggcg cggatgcgtt cgcggcgtgc agcctgctcg
     3181 gcgagccact cgctgcgttc ctgcgccacg agccggacga cgtggcgttc ggatagtccg
     3241 gtgattcgag cgccttcggc ggcggtcacg cgccgctttt tgcggacagt cggctgccgg
     3301 ttgtagccgt cgctgtagcc gtcgctgtag ccgtcgctca tagcaatgcc tccatggctg
     3361 acgcggactt tgcgcgccgc gcaactgtgc tcgccgccgt gcgcgctgct gcgcccttcc
     3421 gcgagatggc cgactggcgc gcactgagtg tggcctcgta gaccacgatc ccgtccgccc
     3481 aaatgcgcga cttggttgtg atccaacgcc aaatgctgtt ggcgatggcg cggacctcgc
     3541 tgtccggtag cggtccggga cacacgtcgt tgcacgggaa ttcggcgttt cgcgcgtggc
     3601 actcggcata gatcgcgcgg ccgagtccgt ccacgttccg ggtcggcagg tagatccgca
     3661 tgagggcggg acgataggcc cacaacctga cggaatcgaa cagtgcgcaa ttccgcccta
     3721 gcggcgtcgg agccgctttg tacgtggtct gctgacgcca gcgcggcggt ggcatgttcg
     3781 cgccgagctc ggcctcgatg tggctgagtg tgtagagatc tgagtggagc cattccgttt
     3841 cccaggcgat gtggccgggg tttttggtca tgaggcctga gtaactgcgg tcgccgtcga
     3901 cggcgcgccg aaggccttcg gcgcacgccg ccatgtatgc gagcggctta cgccgcgcgt
     3961 attcggtgcg tggaacaggg gcgttgagtg cccacactgc gtgtgcgtgg ccgttggcgc
     4021 gattgcccac gatcgcgttg ggcagcggat gggacccccg ggcgctgagc gctcggagcg
     4081 ctgcgtctgg atggtctacg tccacgacca gcaggtttgc cagcgctgtt gggttcgcct
     4141 cgatgtaccg gcggcctagg gccgacgcgc ggctttggcg gtagatcccc tcgagcagat
     4201 cgtcgcttgc cagcggccag tacggcagcc agagctgctc aaattcgtcg gcgacgtggc
     4261 tcacgcttgg tagtagacca cgattaatca ccggtgtatg gtccgacacg agctccaagt
     4321 cagatatttc gctgaggggc caccccacaa ctgcacactc ccccgctctc ccgtcgagcc
     4381 ctggtggtgg aacaccagcg acagccgagc acccccaacc acctgtacca accaggagga
     4441 acacatgcgt cgtttcgagg acgtttccgg gccgctgaga gccgctgtgg cggccgtaca
     4501 cgccgcctta gacccgttag accccctgcc gcctgaatgc gcgggtacga gccacacagc
     4561 gcccgaactt acggagctgg tgggctcacc tggctttatg gcgtacgaat cggctgtgtg
     4621 cgacctgttg ggcgaggtga ggtacgcgct actcacgctg gcaagggcga cacagccgcc
     4681 ccaccgagcc cgcacggccg cgcgcggtgt caacaaccgg gtgagtcgtg cacaccagca
     4741 ggtgttcgag gcttggctcg aagtgcagga catcgtggcg aacgccgccc gatgagccgc
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