Catalog No. PVT11109
Replicon: pUC ori, F1 ori
Plasmid classification: virus series, lentivirus interference vector
Plasmid size: 7484bp
Prokaryotic resistance: Amp
Selection marker: Puro
Clone strain: Stbl3
Culture conditions: 37 C, aerobic LB
Expression host: lactation cells
Induction mode: no need to induce, transient expression.
5'sequencing primers: U6:ATGGACTATCATATGCTTACCGTA
Primers for 3'sequencing: primers designed based on sequences
The MISSION TRC2 Control Vector pLKO-puro is a lentivirus plasmid vector. This vector is in the TRC2 pLKO-puro plasmid backbone, which contains the WPRE. The vector does not contain an shRNA insert and is useful as a negative control in experiments using the TRC2 MISSION shRNA library clones. This allows one to examine the effect of transfection on gene expression and interpret the knockdown effect seen with shRNA clones.