pLP- VSVG

$193.00

  • Model: PVT11128
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pLP-VSVG


Catalog No. PVT11128
Packing 2ug
Function /
Resistance Amp
Screen /
Strain Stbl3
Culture temperature 37degrees centigrade
Replicon pUC
Copy
Promoter CMV
Induction --
Forward primer CMV-F
Reverse primer

 

pLP-VSVG Information

Promoter: CMV

Replicon: pUC ori

Terminator: beta -globin poly (A)

Plasmid classification: virus series, lentivirus packaging carrier

Plasmid size: 5821bp

Prokaryotic resistance: Amp

Cloned strain: Stbl3

Culture conditions: 37 centigrade, aerobic LB

Expression host: mammalian cells

Induction mode: no induction, instantaneous expression

5'sequencing primers: CMV-F:CGCAAATGGGCGGTAGGCGTG

3'sequencing primers: primers designed according to sequence

 

pLP-VSVG Description

pLP-VSVG-vector

 

pLP-VSVG Sequence

LOCUS       Exported                5821 bp ds-DNA     circular SYN 08-SEP-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Untitled
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, September 8, 2016 from SnapGene Viewer 3.1.4
             
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        79..458
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        459..662
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          796..1271
                     /note="beta-globin intron"
                     /note="internally truncated intron from human beta-globin"
     CDS             1346..2881
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /note="VSV-G"
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     polyA_site      3082..3476
                     /note="beta-globin poly(A)"
                     /note="human beta-globin polyadenylation signal"
     rep_origin      complement(3986..4574)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4745..5605)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5606..5710)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 ttggcccatt gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc
       61 caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
      121 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      181 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      241 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      301 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      361 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      421 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      481 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      541 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      601 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      661 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
      721 gaagacaccg ggaccgatcc agcctcccct cgaagcttac atgtggtacc gagctcggat
      781 cctgagaact tcagggtgag tctatgggac ccttgatgtt ttctttcccc ttcttttcta
      841 tggttaagtt catgtcatag gaaggggaga agtaacaggg tacacatatt gaccaaatca
      901 gggtaatttt gcatttgtaa ttttaaaaaa tgctttcttc ttttaatata cttttttgtt
      961 tatcttattt ctaatacttt ccctaatctc tttctttcag ggcaataatg atacaatgta
     1021 tcatgcctct ttgcaccatt ctaaagaata acagtgataa tttctgggtt aaggcaatag
     1081 caatatttct gcatataaat atttctgcat ataaattgta actgatgtaa gaggtttcat
     1141 attgctaata gcagctacaa tccagctacc attctgcttt tattttatgg ttgggataag
     1201 gctggattat tctgagtcca agctaggccc ttttgctaat catgttcata cctcttatct
     1261 tcctcccaca gctcctgggc aacgtgctgg tctgtgtgct ggcccatcac tttggcaaag
     1321 cacgtgagat ctgaattctg acactatgaa gtgccttttg tacttagcct ttttattcat
     1381 tggggtgaat tgcaagttca ccatagtttt tccacacaac caaaaaggaa actggaaaaa
     1441 tgttccttct aattaccatt attgcccgtc aagctcagat ttaaattggc ataatgactt
     1501 aataggcaca gccttacaag tcaaaatgcc caagagtcac aaggctattc aagcagacgg
     1561 ttggatgtgt catgcttcca aatgggtcac tacttgtgat ttccgctggt atggaccgaa
     1621 gtatataaca cattccatcc gatccttcac tccatctgta gaacaatgca aggaaagcat
     1681 tgaacaaacg aaacaaggaa cttggctgaa tccaggcttc cctcctcaaa gttgtggata
     1741 tgcaactgtg acggatgccg aagcagtgat tgtccaggtg actcctcacc atgtgctggt
     1801 tgatgaatac acaggagaat gggttgattc acagttcatc aacggaaaat gcagcaatta
     1861 catatgcccc actgtccata actctacaac ctggcattct gactataagg tcaaagggct
     1921 atgtgattct aacctcattt ccatggacat caccttcttc tcagaggacg gagagctatc
     1981 atccctggga aaggagggca cagggttcag aagtaactac tttgcttatg aaactggagg
     2041 caaggcctgc aaaatgcaat actgcaagca ttggggagtc agactcccat caggtgtctg
     2101 gttcgagatg gctgataagg atctctttgc tgcagccaga ttccctgaat gcccagaagg
     2161 gtcaagtatc tctgctccat ctcagacctc agtggatgta agtctaattc aggacgttga
     2221 gaggatcttg gattattccc tctgccaaga aacctggagc aaaatcagag cgggtcttcc
     2281 aatctctcca gtggatctca gctatcttgc tcctaaaaac ccaggaaccg gtcctgcttt
     2341 caccataatc aatggtaccc taaaatactt tgagaccaga tacatcagag tcgatattgc
     2401 tgctccaatc ctctcaagaa tggtcggaat gatcagtgga actaccacag aaagggaact
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