pLVX- TRE3G Plasmid

$216.00

  • Model: PVT2327
  • 50 Units in Stock
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pLVX-TRE3G

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pLVX-TRE3G,Plasmid pLVX-TRE3G,pLVX-TRE3G vector

pLVX-TRE3G Plasmid information

Promoter: TRE3GV, PGK
Replicon: pUC ori
Terminator: SV40 poly (A) signal
Plasmid classification: virus series, lentivirus cloning vector
Plasmid size: 7819bp
Prokaryotic resistance: Amp
Screening markers: Puro
Cloned strain: Stbl3
Culture conditions: 37 centigrade, aerobic LB
Expression host: mammalian cells
Inducement: tetracycline induction
5'sequencing primers: M13R:CAGGAAACAGCTATGACC
3'sequencing primers: primers designed according to sequence
Use:Lentiviral

pLVX-TRE3G Plasmid  Description

 When used with any Lenti-X Tet-On 3G or Tet-Express inducible expression system, the pLVX-TRE3G Vector Set allows for lentiviral delivery and inducible expression of your gene of interest in a wide variety of mammalian cells. Target cells that contain a gene of interest under the control of a PTRE3GV promoter will express high levels of your gene of interest, when cultured in the presence of doxycycline (when used with the pLVX-Tet3G Vector and a Tet-On 3G system) or in the presence of the Tet-Express tetracycline transactivator (when used with Tet-Express Systems).

pLVX- TRE3G-Plasmid

pLVX-TRE3G Plasmid  Sequence

LOCUS       Exported                7819 bp ds-DNA     circular SYN 29-JAN-2014
DEFINITION  Lentiviral vector for doxycycline-inducible expression of a gene in 
            cells containing the Tet-On 3G transactivator protein.
ACCESSION   .
VERSION     .
KEYWORDS    pLVX-TRE3G
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Clontech
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, September 8, 2016 
 
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     LTR             1..634
                     /note="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /note="HIV-1 psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2028..2144
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2209..2555
                     /note="TRE3GV promoter"
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On 3G; optimized for retroviral
                     and lentiviral vectors"
     protein_bind    2214..2232
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2250..2268
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2286..2304
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2322..2340
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2358..2376
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2394..2412
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2430..2448
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    2556..2599
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        2604..3103
                     /note="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             3124..3723
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    3741..4329
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             4537..5170
                     /note="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(5701..6286)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6457..7317)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
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