pMD2.G Plasmid

$136.00

  • Model: PVT2321
  • 50 Units in Stock
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pMD2.G

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pMD2.G,Plasmid pMD2.G,pMD2.G vector

 

pMD2.G Information

Promoter: CMV

Replicon: pUC ori

Terminator: beta-globin poly (A)

Plasmid classification: virus series, lentiviral packaging carrier

Plasmid size: 5822bp

Plasmid label: VSV-G

Prokaryotic resistance: Amp

Cloned strain: Stbl3

Culture conditions: 37 centigrade, aerobic LB

Expression host: mammalian cells

Induction mode: no induction, instantaneous expression

5'sequencing primers: CMV-F:CGCAAATGGGCGGTAGGCGTG

3'sequencing primers: primers designed according to sequence

Use:Lentiviral

 

pMD2.G Description

PMD2. belong to the VSV-G expression plasmid, and can be used together with pSPAX2.

pMD2.G

 

pMD2.G Sequence

LOCUS       Exported                5822 bp ds-DNA     circular SYN 24-OCT-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Untitled 3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, October 24, 2016 from SnapGene Viewer 3.2.1
 
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_site      1048..1442
                     /note="beta-globin poly(A)"
                     /note="human beta-globin polyadenylation signal"
     rep_origin      complement(1952..2540)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2711..3571)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3572..3676)
                     /gene="bla"
                     /note="AmpR promoter"
     enhancer        3867..4246
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4247..4450
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          4584..5059
                     /note="beta-globin intron"
                     /note="internally truncated intron from human beta-globin"
     CDS             5134..847
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /note="VSV-G"
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
ORIGIN
        1 tggaggcaag gcctgcaaaa tgcaatactg caagcattgg ggagtcagac tcccatcagg
       61 tgtctggttc gagatggctg ataaggatct ctttgctgca gccagattcc ctgaatgccc
      121 agaagggtca agtatctctg ctccatctca gacctcagtg gatgtaagtc taattcagga
      181 cgttgagagg atcttggatt attccctctg ccaagaaacc tggagcaaaa tcagagcggg
      241 tcttccaatc tctccagtgg atctcagcta tcttgctcct aaaaacccag gaaccggtcc
      301 tgctttcacc ataatcaatg gtaccctaaa atactttgag accagataca tcagagtcga
      361 tattgctgct ccaatcctct caagaatggt cggaatgatc agtggaacta ccacagaaag
      421 ggaactgtgg gatgactggg caccatatga agacgtggaa attggaccca atggagttct
      481 gaggaccagt tcaggatata agtttccttt atacatgatt ggacatggta tgttggactc
      541 cgatcttcat cttagctcaa aggctcaggt gttcgaacat cctcacattc aagacgctgc
      601 ttcgcaactt cctgatgatg agagtttatt ttttggtgat actgggctat ccaaaaatcc
      661 aatcgagctt gtagaaggtt ggttcagtag ttggaaaagc tctattgcct cttttttctt
      721 tatcataggg ttaatcattg gactattctt ggttctccga gttggtatcc atctttgcat
      781 taaattaaag cacaccaaga aaagacagat ttatacagac atagagatga accgacttgg
      841 aaagtaactc aaatcctgca caacagattc ttcatgtttg gaccaaatca acttgtgata
      901 ccatgctcaa agaggcctca attatatttg agtttttaat ttttatgaaa aaaaaaaaaa
      961 aaaacggaat tcaccccacc agtgcaggct gcctatcaga aagtggtggc tggtgtggct
     1021 aatgccctgg cccacaagta tcactaagct cgctttcttg ctgtccaatt tctattaaag
     1081 gttcctttgt tccctaagtc caactactaa actgggggat attatgaagg gccttgagca
     1141 tctggattct gcctaataaa aaacatttat tttcattgca atgatgtatt taaattattt
     1201 ctgaatattt tactaaaaag ggaatgtggg aggtcagtgc atttaaaaca taaagaaatg
     1261 aagagctagt tcaaaccttg ggaaaataca ctatatctta aactccatga aagaaggtga
     1321 ggctgcaaac agctaatgca cattggcaac agcccctgat gcctatgcct tattcatccc
     1381 tcagaaaagg attcaagtag aggcttgatt tggaggttaa agttttgcta tgctgtattt
     1441 tacattactt attgttttag ctgtcctcat gaatgtcttt tcactaccca tttgcttatc
     1501 ctgcatctct cagccttgac tccactcagt tctcttgctt agagatacca cctttcccct
     1561 gaagtgttcc ttccatgttt tacggcgaga tggtttctcc tcgcctggcc actcagcctt
     1621 agttgtctct gttgtcttat agaggtctac ttgaagaagg aaaaacaggg ggcatggttt
     1681 gactgtcctg tgagcccttc ttccctgcct cccccactca cagtgacccg gaatccctcg
     1741 acatggcagt ctagcactag tgcggccgca gatctgcttc ctcgctcact gactcgctgc
     1801 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1861 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1921 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1981 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2041 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2101 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     2161 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     2221 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2281 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2341 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     2401 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2461 ccggcaaac
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