pMK4
PVT12369 2ug
pMK4 Search name
pMK4,pMK4 vector,pMK4 plasmid,pMK4 map
pMK4 Information
Bacterial Resistance(s): Amp
Screening: Chl
Growth Strain(s): DH5 alpha
Growth Condition: LB/37 Centigrade

pMK4 Sequence
LOCUS Exported 5585 bp ds-DNA circular SYN 21-SEP-2018
DEFINITION synthetic circular DNA
KEYWORDS pMK4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5585)
AUTHORS Triple Threat
FEATURES Location/Qualifiers
source 1..5585
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 216..518
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/note="lacZ-alpha"
/translation="MTMITPSLAAGRRIPGNSLAVVLQRRDWENPGVTQLNRLAAHPPF
ASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICSDAA"
misc_feature 241..268
/note="MCS"
promoter 759..863
/gene="bla"
/note="AmpR promoter"
CDS 864..1724
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
CDS 3965..4615
/codon_start=1
/note="Chl"
/translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
rep_origin 4815..5403
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgg
241 ctgcaggtcg acggatcccc gggaattcac tggccgtcgt tttacaacgt cgtgactggg
301 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
361 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
421 aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
481 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
541 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
601 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
661 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
721 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
781 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
841 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
901 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
961 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
1021 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
1081 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
1141 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
1201 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
1261 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
1321 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
1381 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
1441 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
1501 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
1561 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
1621 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
1681 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
1741 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
1801 agatccatat ccttcttttt ctgaaccgac ttctcctttt tcgcttcttt attccaattg
1861 ctttattgac gttgagcctc ggaaccctta acaatcccaa aacttgtcga atggtcggct
1921 taatagctca cgctatgccg acattcgtct gcaagtttag ttaagggttc ttctcaacgc
1981 acaataaatt ttctcggcat aaatgcgtgg tctaattttt atttttaata accttgatag
2041 caaaaaatgc cattccaata caaaaccaca tacctataat cgataaccac ataacagtca
2101 taaaaccact cctttttaac aaactttatc acaagaaata tttaaatttt aaatgccttt
2161 attttgaatt ttaaggggca ttttaaagat ttaggggtaa atcatatagt tttatgccta
2221 aaaacctaca gaagctttta aaaagcaaat atgagccaaa taaatatatt ctaattctac
2281 aaacaaaaat ttgagcaaat tcagtgtcga ttttttaaga cactgcccag ttacatgcaa
2341 attaaaattt tcatgatttt ttatagttcc taacagggtt aaaatttgta taacgaaagt
2401 ataatgttta tataacgtta gtataataaa gcattttaac attatacttt tgataatcgt
2461 ttatcgtcgt catcacaata acttttaaaa tactcgtgca taattcaaca gctgacctcc
2521 caataactac atggtgttat cgggaggtca gctgttagca cttatatttt gttattgttc
2581 ttcctcgatt tcgtctatca ttttgtgatt aatttctctt ttttcttgtt ctgttaagtc
2641 ataaagttca ctagctaaat actctttttg tttccaaata taaaaaattt gatagatata
2701 ttcggttgga tcaatttctt ttaagtaatc taaatcccca ttttttaatt tctttttagc
2761 ctctttaaat aatcctgaat aaactaatac ctgtttacct ttaagtgatt tataaaatgc
2821 atcaaagact ttttgattta ttaaataatc actatcttta ccagaatact tagccatttc
2881 atataattct ttattattat tttgtcttat tttttgaact tgaacttgtg ttatttctga
2941 aatgcccgtt acatcacgcc ataaatctaa ccattcttgt tggctaatat aatatctttt
3001 atctgtgaaa tacgatttat ttactgcaat taacacatga aaatgaggat tataatcatc
3061 tcttttttta ttatatgtaa tctctaactt acgaacatat ccctttataa cactacctac
3121 tttttttctc tttataagtt ttctaaaaga attattataa cgttttattt cattttctaa
3181 ttcatcactc attacattag gtgtagtcaa agttaaaaag ataaactcct ttttctcttg
3241 ctgcttaata tattgcatca tcaaagataa acccaatgca tcttttctag cttttctcca
3301 agcacagaca ggacaaaatc gatttttaca agaattagct ttatataatt tctgtttttc
3361 taaagtttta tcagctacaa aagacagaaa tgtattgcaa tcttcaacta aatccatttg
3421 attctctcca atatgacgtt taataaattt ctgaaatact tgatttcttt gttttttctc
3481 agtatacttt tccatgttat aacacataaa aacaacttag ttttcacaaa ctatgacaat
3541 aaaaaaagtt gctttttccc ctttctatgt atgtttttta ctagtcattt aaaacgatac
3601 attaataggt acgaaaaagc aacttttttt gcgcttaaaa ccagtcatac caataactta
3661 agggtaacta gcctcgccgg caatagttac ccttattatc aagataagaa agaaaaggat
3721 ttttcgctac gctcaaatcc tttaaaaaaa cacaaaagac cacatttttt aatgtggtct
3781 tttattcttc aactaaagca cccattagtt caacaaacga aaattggata aagtgggata
3841 tttttaaaat atatatttat gttacagtaa tattgacttt taaaaaagga ttgattctaa
3901 tgaagaaagc agacaagtaa gcctcctaaa ttcactttag ataaaaattt aggaggcata
3961 tcaaatgaac tttaataaaa ttgatttaga caattggaag agaaaagaga tatttaatca
4021 ttatttgaac caacaaacga cttttagtat aaccacagaa attgatatta gtgttttata
4081 ccgaaacata aaacaagaag gatataaatt ttaccctgca tttattttct tagtgacaag
4141 ggtgataaac tcaaatacag cttttagaac tggttacaat agcgacggag agttaggtta
4201 ttgggataag ttagagccac tttatacaat ttttgatggt gtatctaaaa cattctctgg
4261 tatttggact cctgtaaaga atgacttcaa agagttttat gatttatacc tttctgatgt
4321 agagaaatat aatggttcgg ggaaattgtt tcccaaaaca cctatacctg aaaatgcttt
4381 ttctctttct attattccat ggacttcatt tactgggttt aacttaaata tcaataataa
4441 tagtaattac cttctaccca ttattacagc aggaaaattc attaataaag gtaattcaat
4501 atatttaccg ctatctttac aggtacatca ttctgtttgt gatggttatc atgcaggatt
4561 gtttatgaac tctattcagg aattgtcaga taggcctaat gactggcttt tataatatga
4621 gataatgccg actgtacttt ttacagtcgg ttttctaatg tcactaacct gccccgttag
4681 ttgaagaagg tttttatatt acagctccag atctaggtga agatcctttt tgataatctc
4741 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
4801 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
4861 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
4921 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
4981 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
5041 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
5101 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
5161 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
5221 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
5281 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
5341 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
5401 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
5461 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
5521 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
5581 gaaga
//
Caution:
Product is for research use only!