pMP2444
Catalog No. PVT11378
Packing 2ug
pMP2444 Information
Prokaryotic resistance: Gentamicin Gen
Cloning strain: Escherichia coli DH5alpha
Culture conditions: 37 C
pMP2444 Description
PMP2444 is a wide host prokaryotic green fluorescent protein expression plasmid.
pMP2444 Multiple cloning sites
pMP2444 Sequence
LOCUS Exported 5471 bp ds-DNA circular SYN 04-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..5471
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(261..794)
/codon_start=1
/gene="aacC1"
/product="gentamycin acetyltransferase"
/label=Gen
/note="confers resistance to gentamycin"
/translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
EEVMHFDIDPSTAT"
promoter complement(983..1011)
/gene="intI1 (promoter lies within the coding sequence)"
/label=Pc promoter
/note="class 1 integron promoter"
protein_bind 1281..1302
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 1317..1347
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1355..1371
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter 1416..1434
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
misc_feature 1447..1518
/label=MCS
CDS 1520..2239
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS complement(3017..3679)
/codon_start=1
/product="replication protein for the broad-host-range
plasmid pBBR1 from Bordetella bronchiseptica"
/label=pBBR1 Rep
/translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
rep_origin 3680..4449
/label=pBBR1 oriV
/note="replication origin of the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica; requires the pBBR1
Rep protein for replication"
ORIGIN
1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
181 aaaagtgcca cctggcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg
241 atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact
301 tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct
361 tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgaggag attgatgagc
421 gcggtggcaa tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc
481 tcactacgcg gctgctcaaa cctgggcaga acgtaagccg cgagagcgcc aacaaccgct
541 tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa
601 tcggagtccg gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga
661 tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg
721 cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
781 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
841 ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa
901 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga
961 gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtca actgggttcg
1021 tgccttcatc cgtttccacg gtgtgcgtcc atgggcaaat attatacgca aggcgacaag
1081 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
1141 agaatgctta atgaattaca acagttttta tgcatgcgcc caatacgcaa accgcctctc
1201 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
1261 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
1321 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
1381 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
1441 aagctgggta ccgggccccc cctcgaggtc gacggtatcg ataagcttga tatcgaattc
1501 ctgcagcccg ggggatccaa tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
1561 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
1621 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
1681 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
1741 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
1801 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
1861 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
1921 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
1981 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
2041 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
2101 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
2161 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
2221 ggacgagctg tacaagtagt ctagattagt ggagctccaa ttcgccctat agtgagtcgt
2281 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
2341 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
2401 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg aaat
