pmScarlet-C1
Catalog No. PVT10762
Packing 2ug
pmScarlet-C1 Information
Function Mammalian fluorescent plasmid
Resistance /
Screen /
Strain /
Culture temperature
Replicon
Copy
Promoter
Induction
Forward primer
Reverse primer
pmScarlet-C1 Description-- pmScarlet-C1 vector polyclonal site
pmScarlet-C1 Sequence
LOCUS Exported 4710 bp ds-DNA circular SYN 29-NOV-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pmScarlet-C1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4710)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Tuesday, November 29, 2016 from SnapGene Viewer 3.2.1
FEATURES Location/Qualifiers
source 1..4710
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 613..1300
/note="mScarlet"
misc_feature 1309..1374
/note="MCS"
/note="multiple cloning site"
polyA_signal 1498..1619
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1626..2081)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2108..2212
/gene="bla"
/note="AmpR promoter"
promoter 2214..2571
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2422..2557
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2606..3400
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3632..3679
/note="HSV TK poly(A) signal"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4008..4596
/direction=RIGHT
/note="pUC ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggtgag caagggcgag gcagtgatca aggagttcat gcggttcaag
661 gtgcacatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
721 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
781 ttctcctggg acatcctgtc ccctcagttc atgtacggct ccagggcctt caccaagcac
841 cccgccgaca tccccgacta ctataagcag tccttccccg agggcttcaa gtgggagcgc
901 gtgatgaact tcgaggacgg cggcgccgtg accgtgaccc aggacacctc cctggaggac
961 ggcaccctga tctacaaggt gaagctccgc ggcaccaact tccctcctga cggccccgta
1021 atgcagaaga agacaatggg ctgggaagcg tccaccgagc ggttgtaccc cgaggacggc
1081 gtgctgaagg gcgacattaa gatggccctg cgcctgaagg acggcggccg ctacctggcg
1141 gacttcaaga ccacctacaa ggccaagaag cccgtgcaga tgcccggcgc ctacaacgtc
1201 gaccgcaagt tggacatcac ctcccacaac gaggactaca ccgtggtgga acagtacgaa
1261 cgctccgagg gccgccactc caccggcggc atggacgagc tgtacaagtc cggactcaga
1321 tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg atccaccgga
1381 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
1441 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
1501 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
1561 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
1621 cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
1681 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
1741 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
1801 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
1861 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
1921 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
1981 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
2041 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
2101 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
2161 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc
2221 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
2281 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
2341 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
2401 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
2461 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
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