pMT- V5- HisA

$228.00

  • Model: PVT11314
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pMT-V5-HisA


Catalog No. PVT11314
Packing 2ug
Function /
Resistance Amp
Screen /
Strain DH5alpha
Culture temperature 37degrees centigrade

 

pMT-V5-HisA Description

pMT/V5-His is a 3.5 kb expression vector designed for use with the Drosophila Inducible Expression System. Upon transfection, the vector allows transient, inducible expression of your protein of interest in Drosophila cells. When cotransfected with the selection vectors, pCoHygro or pCoBlast, included with the appropriate DES® Inducible Kit, pMT/V5-His allows selection of stable cell lines exhibiting inducible expression of the protein of interest.

 

pMT-V5-HisA Multiple cloning site

pMT-V5-HisA-vector

 

pMT-V5-HisA Sequence

LOCUS       Exported                3538 bp ds-DNA     circular SYN 26-NOV-2017
DEFINITION  synthetic circular DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3538)
  TITLE     Direct Submission
           
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        407..833
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     CDS             930..971
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             981..998
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    1040..1174
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1720..2308)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2479..3339)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3340..3444)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccag tgaattaatt cgttgcagga
      421 caggatgtgg tgcccgatgt gactagctct ttgctgcagg ccgtcctatc ctctggttcc
      481 gataagagac ccagaactcc ggccccccac cgcccaccgc cacccccata catatgtggt
      541 acgcaagtaa gagtgcctgc gcatgcccca tgtgccccac caagagcttt gcatcccata
      601 caagtcccca aagtggagaa ccgaaccaat tcttcgcggg cagaacaaaa gcttctgcac
      661 acgtctccac tcgaatttgg agccggccgg cgtgtgcaaa agaggtgaat cgaacgaaag
      721 acccgtgtgt aaagccgcgt ttccaaaatg tataaaaccg agagcatctg gccaatgtgc
      781 atcagttgtg gtcagcagca aaatcaagtg aatcatctca gtgcaactaa aggggggatc
      841 tagatcgggg tacctactag tccagtgtgg tggaattctg cagatatcca gcacagtggc
      901 ggccgctcga gtctagaggg cccttcgaag gtaagcctat ccctaaccct ctcctcggtc
      961 tcgattctac gcgtaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag
     1021 cctcgactgt gccttctaag atccagacat gataagatac attgatgagt ttggacaaac
     1081 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     1141 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     1201 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     1261 tggtatggct gattatgatc agtcgacctg caggcatgca agcttggcgt aatcatggtc
     1321 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     1381 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     1441 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     1501 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     1561 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     1621 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     1681 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     1741 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     1801 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     1861 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     1921 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1981 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2041 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     2101 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     2161 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     2221 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     2281 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     2341 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     2401 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     2461 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     2521 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     2581 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     2641 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     2701 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     2761 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     2821 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     2881 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     2941 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     3001 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     3061 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     3121 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     3181 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     3241 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     3301 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     3361 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     3421 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     3481 aaccattatt atcatgacat taacctataa aaataggcgt atc
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